Involvement of reverse transcription in the replication of cauliflower mosaic virus: A detailed model and test of some aspects

Pfeiffer, P.; Höhn, Thomas

doi:10.1016/0092-8674(83)90020-x

Cited by 206 publications

(127 citation statements)

References 58 publications

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“…1). These features and their distribution are similar to those of CaMV, where the tRNA Met binds to the primary transcript and acts as the primer for the synthesis of the positive-sense DNA strand during replication (Hull & Covey, 1983;Guilley et al, 1983;Pfeiffer & Hohn, 1983). For agroinfection with CaMV, it is necessary to have a 'one-and-a-bitmer" clone, comprising an uninterrupted sequence giving rise to the full-length 35S transcript with terminal repeats (Grimsley & Bisaro, 1987).…”

Section: Discussionmentioning

confidence: 99%

Rice tungro bacilliform virus DNA independently infects rice after Agrobacterium-mediated transfer

Dasgupta

Hull

Eastop

et al. 1991

Journal of General Virology

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In nature, rice tungro disease is caused by an RNA and a DNA virus complex, but we have obtained an independently infectious clone of rice tungro bacilliform virus (RTBV) DNA. Infectivity could be demonstrated only when a more than unit-length copy was cloned in the Agrobacterium binary vector Binl9 and agroinoculated into rice plants. Rice plants thus agroinfected with cloned RTBV DNA showed typical symptoms of tungro disease, presence of viral DNA and bacilliform particles, and could be used as a source of virus to infect healthy plants by the green leafhopper (Nephotettix virescens). The importance of this infectious clone in understanding the molecular biology of RTBV and the rice tungro disease is discussed.

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Section: Discussionmentioning

confidence: 99%

Rice tungro bacilliform virus DNA independently infects rice after Agrobacterium-mediated transfer

Dasgupta

Hull

Eastop

et al. 1991

Journal of General Virology

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“…Reverse transcriptase activity may be provided in trans in plant nuclei either by endogenous elements like Cin4 (11) or by nonintegrating, reverse transcribed viruses like CaMV (22). In potato, an intronless pseudogene derived from actin mRNA has been discovered recently (32).…”

Section: Resultsmentioning

confidence: 99%

“…Initiator methionyl-tRNA sequences are highly conserved in nature (20,21), and it is likely that the maize initiator methionyl-tRNA has an identical 3' sequence. Reverse transcriptions of Ty, copia, and CaMV (22) RNAs are primed by initiator methionyl-tRNAs.…”

Section: Resultsmentioning

confidence: 99%

Structure and coding properties of Bs1, a maize retrovirus-like transposon.

Jin

Bennetzen²

1989

Proc. Natl. Acad. Sci. U.S.A.

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We have sequenced Bs), an insertion element isolated from a null allele of the Adhi locus encoding alcohol dehydrogenase in maize. The Bs) element is 3203 base pairs (bp) in length, has 302-bp identical long terminal direct repeats (LTRs), and created a 5-bp flanking direct duplication oftarget AdhI DNA upon insertion. The 5' LTR is followed by a canonical primer binding site with homology to the plant initiator methionyl-tRNA, and the 3' LTR is directly preceded by a polypurine stretch like that observed in retroviruses and retrotransposons. Bs) encodes two overlapping open reading frames specifying peptides of 740 and 168 amino acids. The longer open reading frame specifies a peptide with amino acid homology to the protease and nucleic acid binding moiety of retroviruses and retrotransposons. The deduced amino acid sequence encoded by Bsl lacks convincing homology to the polymerase (reverse transcriptase) encoded by retroposons, despite the fact that this polymerase-encoding domain is routinely the most conserved region of any such element. The sequence and relatively small size of Bs) suggest that this element is a deleted retrotransposon that inserted into AdhM with the aid of a reverse transcriptase function provided in trans. In vitro transcribed Bsl complementary RNA was translated in vitro to produce both a protein of 81 kDa representing open reading frame 1 (ORF1) and one of the 95-kDa size predicted for the frame-shifted fusion of ORF1 and ORF2. As with many other retroposons, the efficiency of translational initiation at the AUG beginning ORF1 was not noticeably affected by the presence of one or more upstream, unproductive AUGs in the complementary RNA transcript.By both structural and mechanistic criteria, eukaryotic insertion sequences and transposable elements can be divided into two major categories. One class of elements is bounded by inverted terminal repeat sequences and transposes through excision (1, 2) and/or enhanced replication (3) of the integrated element. Examples of this class of transposable elements are the controlling elements of maize (4) and the P elements of Drosophila (5). A second class of eukaryotic insertion sequences, the "retroposons," integrate into the chromosome after reverse transcription of element-encoded RNA (6), are particularly abundant in animals, and are the only type of insertion sequence yet discovered in fungi. Both the retroviruses (6) and the LI elements (7) of vertebrates and the "retrotransposons" Ty of yeast (8) and copia of Drosophila (9) fall into this latter category.Despite the early discovery and characterization of numerous distinct transposable elements in maize (1, 4), a canonical retrovirus or retrotransposon has not been described in plants. The DNA caulimoviruses ofplants have the structural properties and encode the reverse transcriptase activity definitional of a retrovirus or retrotransposon but apparently do not integrate into chromosomal DNA (10). Recently, Schwarz-Sommer et al. (11) have found that the Cin4 insertion element of m...

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“…It is the major component of the amorphous inclusion bodies accumulating in infected cells (Odell and Howell, 1980;Covey and Hull, 1981). lnclusion bodies have been suggested to be the site of translation of CaMV RNA, accumulation of vira1 translation products (De Zoeten et al, 1989), reverse transcription (Pfeiffer and Hohn, 1983;Bonneville et al, 1984;Mazzolini et al, 1985;Thomas et al, 1985), and virus assembly. In electron micrographs, polysomes are seen attached to the surface of inclusion bodies, whereas virus particles are inside (Shepherd et al, 1979).…”

Section: Introductionmentioning

confidence: 99%

Cauliflower Mosaic Virus Gene VI Controls Translation from Dicistronic Expression Units in Transgenic Arabidopsis Plants.

Zijlstra

Höhn

1992

Plant Cell

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Involvement of reverse transcription in the replication of cauliflower mosaic virus: A detailed model and test of some aspects

Cited by 206 publications

References 58 publications

Rice tungro bacilliform virus DNA independently infects rice after Agrobacterium-mediated transfer

Rice tungro bacilliform virus DNA independently infects rice after Agrobacterium-mediated transfer

Structure and coding properties of Bs1, a maize retrovirus-like transposon.

Cauliflower Mosaic Virus Gene VI Controls Translation from Dicistronic Expression Units in Transgenic Arabidopsis Plants.

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