Inward rectifier K+ channel Kir2.3 (IRK3) in reactive astrocytes from adult rat brain

Perillan, P. R.; Li, Xing; Potts, E. A.; Chen, Mingkui; Bredt, David S.; Simard, J. Marc

doi:10.1002/1098-1136(200008)31:2<181::aid-glia90>3.0.co;2-8

Cited by 30 publications

(29 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2 B, a), consistent with previous observations in primary cultured cells of the same origin (Perillan et al, 1999(Perillan et al, , 2000. Inward currents negative to the K ϩ equilibrium potential (E K ) were usually Ͻ100 pA, much smaller than values reported in cultured neonatal astrocytes (Ransom and Sontheimer, 1995) but consistent with findings in astrocytes freshly isolated from injured brain (Bordey and Sontheimer, 1998;Schroder et al, 1999).…”

Section: General Electrophysiological Properties Of Nrassupporting

confidence: 89%

“…A subgroup of phase-bright cells had multiple short but distinct cell processes that were shorter than the cell soma. E xcept for occasional red blood cells, Ͼ95% of cells were GFAP-positive when examined by immunofluorescence (Perillan et al, 1999(Perillan et al, , 2000. For the experiments reported here, we studied only larger (Ϸ30 m diameter), phase-bright cells with short processes (less than one cell length).…”

Section: Methodsmentioning

confidence: 99%

“…We studied freshly isolated native reactive astrocytes (NRAs) from adult rat brain, a model system similar to that characterized previously by us (Perillan et al, 1999(Perillan et al, , 2000, except that cells were not cultured but were stored at 4°C until studied, within 24 hr of isolation from the brain. Here we report that NRAs from adult brain express a 35 pS nonselective cation channel that is activated by depletion of [ATP] i at physiological concentrations of [Ca 2ϩ ] i .…”

Section: Abstract: Cation Channel; Ca 2ϩ ; Atp; Cell Swelling; Astromentioning

confidence: 99%

See 2 more Smart Citations

Cell Swelling and a Nonselective Cation Channel Regulated by Internal Ca²⁺and ATP in Native Reactive Astrocytes from Adult Rat Brain

Chen¹,

2001

Self Cite

View full text Add to dashboard Cite

Hypoxia-ischemia and ATP depletion are associated with glial swelling and blebbing, but mechanisms involved in these effects remain incompletely characterized. We examined morphological and electrophysiological responses of freshly isolated native reactive astrocytes (NRAs) after exposure to NaN 3 , which depletes cellular ATP. Here we report that NaN 3 caused profound and sustained depolarization attributable to activation of a novel 35 pS Ca 2ϩ -activated, [ATP] i -sensitive nonselective cation (NC Ca-ATP ) channel, found in Ͼ90% of excised membrane patches. The channel was impermeable to Cl Ϫ , was nearly equally permeable to monovalent cations, with permeabilities relative to K ϩ being P Cs .12, 0.31, and 1.5 M at Ϫ40, Ϫ80, and Ϫ120 mV, respectively), with a Hill coefficient of 1.5. Channel opening by [ATP] i depletion was accompanied by and appeared to precede blebbing of the cell membrane, suggesting participation of this channel in cation flux involved in cell swelling. We conclude that NRAs from adult rat brain express a 35 pS NC Ca-ATP channel that may play an important role in the pathogenesis of brain swelling.

show abstract

Section: General Electrophysiological Properties Of Nrassupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Abstract: Cation Channel; Ca 2ϩ ; Atp; Cell Swelling; Astromentioning

confidence: 99%

See 1 more Smart Citation

Cell Swelling and a Nonselective Cation Channel Regulated by Internal Ca²⁺and ATP in Native Reactive Astrocytes from Adult Rat Brain

Chen¹,

2001

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, Kir2.3 has been identified in the astrocytes from adult rat brain [14]. In the present study, glial cells that immunoreactive for Kir1.1, Kir2.3 and Kir4.1 were also immunoreactive for GFAP but not for CNPase.…”

Section: Rt-pcrsupporting

confidence: 50%

Immunohistochemical Distribution of Inwardly Rectifying K+ Channels in the Medulla Oblongata of the Rat

et al. 2008

View full text Add to dashboard Cite

ABSTRACT. The inwardly rectifying K + channels, Kir1.1, Kir2.3 and Kir4.1-Kir5.1, are the candidate chemosensory molecules for CO 2 / H + . We determined the mRNA expression and immunohistochemical localization of these channels in the medulla oblongata of the rat. RT-PCR analysis revealed mRNAs of Kir1.1, Kir2.3, Kir4.1 and Kir5.1 were detected in the medulla. The immunoreactivities for Kir1.1, Kir2.3, Kir4.1, and Kir5.1 were observed in the medulla, and immunolabeling pattern was varied by the subunit. Immunoreactivities for Kir1.1 and Kir2.3 were observed in the nerve cell bodies and glial cells both in the chemosensory areas [nucleus tractus solitarius (NTS), nucleus raphe obscurus (RO), pre-Bötzinger complex (PreBötC)] and non-chemosensory area [hypoglossal nucleus (XII), inferior olive nucleus (IO)]. Kir4.1 immunoreactivity was observed in the glial cells and neuropil, especially in XII and IO. Kir5.1 immunoreactivity was observed in the nerve cell bodies in the XII, RO, and PreBötC, but not in the NTS or IO. In the NTS, a dense network of varicose nerve fibers showed immunoreactivity for Kir5.1. Our findings suggest that Kir channels may not act specific to the central chemoreception, but regulate the ionic properties of cellular membranes in various neurons and glial cells. KEY WORDS: chemoreceptor, hypercapnic acidosis, inwardly rectifying K + channel, medulla oblongata.

show abstract

“…Experiments were carried out assuming E mϭ Ϫ67 mV. After single-channel data collection, the recording was converted from a cell-attached to a conventional whole cell configuration to measure E m. The mean value of Em measurement was used to compute the proper value of E rev for the single-channel recordings (23).…”

Section: Methodsmentioning

confidence: 99%

EGF and HB-EGF modulate inward potassium current in human bladder urothelial cells from normal and interstitial cystitis patients

Sun¹,

Chen²,

Lowentritt³

et al. 2007

American Journal of Physiology-Cell Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

Inward rectifier K+ channel Kir2.3 (IRK3) in reactive astrocytes from adult rat brain

Cited by 30 publications

References 60 publications

Cell Swelling and a Nonselective Cation Channel Regulated by Internal Ca²⁺and ATP in Native Reactive Astrocytes from Adult Rat Brain

Cell Swelling and a Nonselective Cation Channel Regulated by Internal Ca²⁺and ATP in Native Reactive Astrocytes from Adult Rat Brain

Immunohistochemical Distribution of Inwardly Rectifying K+ Channels in the Medulla Oblongata of the Rat

EGF and HB-EGF modulate inward potassium current in human bladder urothelial cells from normal and interstitial cystitis patients

Contact Info

Product

Resources

About

Inward rectifier K+ channel Kir2.3 (IRK3) in reactive astrocytes from adult rat brain

Cited by 30 publications

References 60 publications

Cell Swelling and a Nonselective Cation Channel Regulated by Internal Ca2+and ATP in Native Reactive Astrocytes from Adult Rat Brain

Cell Swelling and a Nonselective Cation Channel Regulated by Internal Ca2+and ATP in Native Reactive Astrocytes from Adult Rat Brain

Immunohistochemical Distribution of Inwardly Rectifying K+ Channels in the Medulla Oblongata of the Rat

EGF and HB-EGF modulate inward potassium current in human bladder urothelial cells from normal and interstitial cystitis patients

Contact Info

Product

Resources

About

Cell Swelling and a Nonselective Cation Channel Regulated by Internal Ca²⁺and ATP in Native Reactive Astrocytes from Adult Rat Brain

Cell Swelling and a Nonselective Cation Channel Regulated by Internal Ca²⁺and ATP in Native Reactive Astrocytes from Adult Rat Brain