Iodinated Contrast Media Differentially Affect Afferent and Efferent Arteriolar Tone and Reactivity in Mice: A Possible Explanation for Reduced Glomerular Filtration Rate

Liu, Zhi Z; Viegas, Vinícius Urbano; Perlewitz, Andrea; Lai, En Yin; Persson, Pontus B.; Patzak, Andreas; Sendeski, Mauricio

doi:10.1148/radiol.12120044

Cited by 44 publications

(35 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The groups which received CM had an end concentration of 23 mg iodine/ml (1.8*10 −4 mol/l) (iodixanol, GE Healthcare, Munich, Germany) in the intraluminal solution. The CM concentration is the same shown to cause constriction of renal vasa recta and afferent arterioles, and is within the range possibly reached during intravascular procedures in humans 15,19,20 .…”

Section: Intraluminalsupporting

confidence: 62%

Effects of iodinated contrast media in a novel model for cerebral vasospasm

Nikitina

Zavaritskaya

Semenyutin

et al. 2015

Arq. Neuro-Psiquiatr.

Self Cite

View full text Add to dashboard Cite

Objective: We developed an in vitro model for vasospasm post subarachnoid hemorrhage that was suitable for investigating brain vessel autoregulation. We further investigated the effects of iodinated contrast medium on the vascular tone and the myogenic response of spastic cerebral vessels. Method: We isolated and perfused the superior cerebellar arteries of rats. The vessels were pressurized and studied under isobaric conditions. Coagulated blood was used to simulate subarachnoid hemorrhage. The contrast medium iodixanol was applied intraluminally. Results: Vessels exposed to blood developed significantly stronger myogenic tone (65.7 ± 2.0% vs 77.1 ± 1.2% of the maximum diameter, for the blood and the control group, respectively) and significantly decreased myogenic response, compared with the control groups. The contrast medium did not worsen the myogenic tone or the myogenic response in any group. Conclusion: Our results show that deranged myogenic response may contribute to cerebral blood flow disturbances subsequent to subarachnoid hemorrhage. The contrast medium did not have any negative influence on vessel tone or myogenic response in this experimental setting.Keywords: brain ischemia, cerebral angiography, contrast media, hemodynamics, intracranial aneurysm, intracranial vasospasm, subarachnoid hemorrhage. RESUMOObjetivo: Desenvolvemos um modelo in vitro para vasoespasmo subsequente à hemorragia subaracnóide que foi adequado para investigar a autorregularão dos vasos cerebrais. Em seguida investigamos os efeitos o meio de contraste iodado no tônus vascular e na resposta miogênica dos vasos cerebrais espásticos. Método: Isolamos e perfundimos as artérias cerebelares superiores de ratos. Os vasos foram pressurizados e estudados em condições isobáricas. Sangue coagulado foi utilizado para simular hemorragia subaracnóide. O meio de contraste iodixanol foi aplicado intraluminarmente. Resultados: Os vasos expostos ao sangue desenvolveram aumento significativo do tônus miogênico (65.7 ± 2.0% vs 77.1 ± 1.2% do maior diâmetro, para o grupo de sangue e o grupo controle, respectivamente) com resposta miogênica significativamente menor do que aquela dos controles. O meio de contraste iodado não piorou o tônus miogênico ou a resposta miogênica em nenhum dos grupos. Conclusão: Nossos resultados mostram que uma resposta miogênica pode contribuir para as alterações de fluxo sanguíneo cerebral subsequentes à hemorragia subaracnóide. O meio de contraste iodado não teve nenhuma influência negativa no tônus vascular ou na resposta miogênica neste modelo experimental.Palavras-chave: isquemia cerebral, angiografia cerebral, meio de contraste, hemodinâmica, aneurisma intracraniano, vasoespasmo intracraniano, hemorragia subaracnóide.Cerebral delayed vasospasm is a severe complication following spontaneous subarachnoid hemorrhage (SAH). Vasospasm is an important cause of death and contributes 10-12% to the overall mortality after SAH, which reaches approximately 50% within the first month 1,2,3 .There is nowadays no speci...

show abstract

Section: Intraluminalsupporting

confidence: 62%

Effects of iodinated contrast media in a novel model for cerebral vasospasm

Nikitina

Zavaritskaya

Semenyutin

et al. 2015

Arq. Neuro-Psiquiatr.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Up to our knowledge, our experiments are the first direct evidence that increased TGF might contribute to the decreases in medullary perfusion due to CM observed in vivo-in addition to other factors such as 1) direct DVR constriction by CM due to endothelial damage and dysfunction (37)(38)(39), 2) a more marked constrictive effect of CM in afferent compared with efferent arterioles (27), and 3) properties of CM such as high viscosity or extremes of osmolarity (35). Importantly, in our setting the concentration of CM reaching the macula densa is kept constant.…”

Section: Production Of Omentioning

confidence: 59%

Iodinated contrast media cause direct tubular cell damage, leading to oxidative stress, low nitric oxide, and impairment of tubuloglomerular feedback

Liu

Schmerbach

Lü

et al. 2014

American Journal of Physiology-Renal Physiology

Self Cite

View full text Add to dashboard Cite

Iodinated contrast media (CM) have adverse effects that may result in contrast-induced acute kidney injury. Oxidative stress is believed to play a role in CM-induced kidney injury. We test the hypothesis that oxidative stress and reduced nitric oxide in tubules are consequences of CM-induced direct cell damage and that increased local oxidative stress may increase tubuloglomerular feedback. Rat thick ascending limbs (TAL) were isolated and perfused. Superoxide and nitric oxide were quantified using fluorescence techniques. Cell death rate was estimated using propidium iodide and trypan blue. The function of macula densa and tubuloglomerular feedback responsiveness were measured in isolated, perfused juxtaglomerular apparatuses (JGA) of rabbits. The expression of genes related to oxidative stress and the activity of superoxide dismutase (SOD) were investigated in the renal medulla of rats that received CM. CM increased superoxide concentration and reduced nitric oxide bioavailability in TAL. Propidium iodide fluorescence and trypan blue uptake increased more in CM-perfused TAL than in controls, indicating increased rate of cell death. There were no marked acute changes in the expression of genes related to oxidative stress in medullary segments of Henle's loop. SOD activity did not differ between CM and control groups. The tubuloglomerular feedback in isolated JGA was increased by CM. Tubular cell damage and accompanying oxidative stress in our model are consequences of CM-induced direct cell damage, which also modifies the tubulovascular interaction at the macula densa, and may therefore contribute to disturbances of renal perfusion and filtration.

show abstract

“…Though the detailed mechanism of contrast medium-induced AKI is not fully understood, it is closely related to the vascular dysfunction in addition to the direct toxic effect in renal tubular cells. Contrast medium has been reported to have vasoconstriction effect, resulting in the reduction of vasa recta blood supply and decreases in the blood flow rate in afferent arteriole, which further lead to renal tubular damage and decline of glomerular filtration 191–193 . The pre-existence of vascular dysfunction in CKD can certainly augment these pathological changes, inducing more severe AKI.…”

Section: Potential Mechanisms Underlying the Susceptibility And mentioning

confidence: 99%

AKI on CKD: heightened injury, suppressed repair, and the underlying mechanisms

Wei

Liu

et al. 2017

Kidney International

325

222

View full text Add to dashboard Cite

Acute kidney injury (AKI) and chronic kidney disease (CKD) are inter-connected. While AKI-to-CKD transition has been intensively studied, the information of AKI on CKD is very limited. Nonetheless, AKI, when occurring in CKD patients, is known to be more severe and difficult to recover. CKD is associated with significant changes in cell signaling in kidney tissues, including the activation of TGF-β, p53, HIF, and major developmental pathways. At the cellular level, CKD is characterized by mitochondrial dysfunction, oxidative stress, and aberrant autophagy. At the tissue level, CKD is characterized by chronic inflammation and vascular dysfunction. These pathological changes may contribute to the heightened sensitivity of, and non-recovery from, AKI in CKD patients.

show abstract

Iodinated Contrast Media Differentially Affect Afferent and Efferent Arteriolar Tone and Reactivity in Mice: A Possible Explanation for Reduced Glomerular Filtration Rate

Cited by 44 publications

References 27 publications

Effects of iodinated contrast media in a novel model for cerebral vasospasm

Effects of iodinated contrast media in a novel model for cerebral vasospasm

Iodinated contrast media cause direct tubular cell damage, leading to oxidative stress, low nitric oxide, and impairment of tubuloglomerular feedback

AKI on CKD: heightened injury, suppressed repair, and the underlying mechanisms

Contact Info

Product

Resources

About