Ion Channels in Anesthesia

Zhou, Wei; Guan, Zhonghui

doi:10.1007/978-981-16-4254-8_19

Cited by 6 publications

(6 citation statements)

References 84 publications

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“…γ-Aminobutyric acid subtype receptors, a kind of pentameric ligand-gated chloride ion channel receptor, are the most prominent mediators of inhibitory signals in the central nervous system and play a pivotal role in anesthesia ( Zhou and Guan, 2021 ). GABA A receptors are heteropentamers, consisting of subunits from six α subunits (α1-6), three ß subunits (β1-3), three γ subunits (γ1-3), and others ( Zhu et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…The role of GABA A receptors in inhibitory currents is influenced by subunit composition and arrangement ( Minier and Sigel, 2004 ; Hannan et al, 2020 ). The mature receptor has a central chloride ion channel that is gated by the neurotransmitter GABA and is regulated by many general anesthetics, such as propofol and etomidate ( Zhou and Guan, 2021 ; Ghit et al, 2021 ; Chen Q., et al, 2018 ; Alvarez and Pecci, 2018 ). GABA A receptors are mainly distributed in the postsynaptic of synapse, where GABA neurotransmitters can bind to GABA A receptors, causing chloride ion channels to open and increasing intracellular anions in milliseconds, leading to cell membrane depolarization, thus mediating the transmission of inhibitory signals.…”

Section: Discussionmentioning

confidence: 99%

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Central and peripheral analgesic active components of triterpenoid saponins from Stauntonia chinensis and their action mechanism

Gong,

Zhang,

et al. 2023

Front. Pharmacol.

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Triterpenoid saponins from Stauntonia chinensis have been proven to be a potential candidate for inflammatory pain relief. Our pharmacological studies confirmed that the analgesic role of triterpenoid saponins from S. chinensis occurred via a particular increase in the inhibitory synaptic response in the cortex at resting state and the modulation of the capsaicin receptor. However, its analgesic active components and whether its analgesic mechanism are limited to this are not clear. In order to further determine its active components and analgesic mechanism, we used the patch clamp technique to screen the chemical components that can increase inhibitory synaptic response and antagonize transient receptor potential vanilloid 1, and then used in vivo animal experiments to evaluate the analgesic effect of the selected chemical components. Finally, we used the patch clamp technique and molecular biology technology to study the analgesic mechanism of the selected chemical components. The results showed that triterpenoid saponins from S. chinensis could enhance the inhibitory synaptic effect and antagonize the transient receptor potential vanilloid 1 through different chemical components, and produce central and peripheral analgesic effects. The above results fully reflect that “traditional Chinese medicine has multi-component, multi-target, and multi-channel synergistic regulation”.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Central and peripheral analgesic active components of triterpenoid saponins from Stauntonia chinensis and their action mechanism

Gong,

Zhang,

et al. 2023

Front. Pharmacol.

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“…Most K 2P channels show enhanced conductance under the influence of volatile anesthetics (VA) [98]. As a consequence of elevated channel function, hyperpolarization of excitable neurons is induced contributing to the mechanism of general anesthesia [99]. Mutational studies at TASK-3 channels identified a possible binding site formed by residues of M2 and M3 at the intracellular site enabling stabilization of an open channel state [100].…”

Section: Tresk Channel Activatorsmentioning

confidence: 99%

The Special One: Architecture, Physiology and Pharmacology of the TRESK Channel

Schreiber

Düfer

Seebohm

2022

Cell Physiol Biochem

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The TWIK-related spinal cord K+ channel (TRESK) is part of the two-pore domain K+ channel family (K2P), which are also called leak potassium channels. As indicated by the channel family name, TRESK conducts K+ ions along the concentration gradient in a nearly voltage-independent manner leading to lowered membrane potentials. Although functional and pharmacological similarities exist, TRESK shows low sequence identity with other K2P channels. Moreover, the channel possesses several unique features such as its sensitivity to intracellular Ca2+ ions, that are not found in other K2P channels. High expression rates are found in immune-associated and neuronal cells, especially in sensory neurons of the dorsal root and trigeminal ganglia. As a consequence of the induced hyperpolarization, TRESK influences neuronal firing, the release of inflammatory mediators and the proliferation of distinct immune cells. Consequently, this channel might be a suitable target for pharmacological intervention in migraine, epilepsy, neuropathic pain or distinct immune diseases. In this review, we summarize the biochemical and biophysical properties of TRESK channels as well as their sensitivity to different known compounds. Furthermore, we give a structured overview about the physiological and pathophysiological impact of TRESK, that render the channel as an interesting target for specific drug development.

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“…Given that ion channels are fundamental components of all cells, both in excitable and non-excitable cells [ 27 , 28 ], in this work, we focused on analyzing whether ouabain induces changes in the expression of endogenous voltage-gated potassium channels of MDCK cells.…”

Section: Introductionmentioning

confidence: 99%

Ouabain-Induced Changes in the Expression of Voltage-Gated Potassium Channels in Epithelial Cells Depend on Cell–Cell Contacts

Cereijido

Jimenez

Hinojosa

et al. 2022

IJMS

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Ouabain is a cardiac glycoside, initially isolated from plants, and currently thought to be a hormone since some mammals synthesize it endogenously. It has been shown that in epithelial cells, it induces changes in properties and components related to apical–basolateral polarity and cell–cell contacts. In this work, we used a whole-cell patch clamp to test whether ouabain affects the properties of the voltage-gated potassium currents (Ik) of epithelial cells (MDCK). We found that: (1) in cells arranged as mature monolayers, ouabain induced changes in the properties of Ik; (2) it also accelerated the recovery of Ik in cells previously trypsinized and re-seeded at confluence; (3) in cell–cell contact-lacking cells, ouabain did not produce a significant change; (4) Na+/K+ ATPase might be the receptor that mediates the effect of ouabain on Ik; (5) the ouabain-induced changes in Ik required the synthesis of new nucleotides and proteins, as well as Golgi processing and exocytosis, as evidenced by treatment with drugs inhibiting those processes; and (5) the signaling cascade included the participation of cSrC, PI3K, Erk1/2, NF-κB and β-catenin. These results reveal a new role for ouabain as a modulator of the expression of voltage-gated potassium channels, which require cells to be in contact with themselves.

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Ion Channels in Anesthesia

Cited by 6 publications

References 84 publications

Central and peripheral analgesic active components of triterpenoid saponins from Stauntonia chinensis and their action mechanism

Central and peripheral analgesic active components of triterpenoid saponins from Stauntonia chinensis and their action mechanism

The Special One: Architecture, Physiology and Pharmacology of the TRESK Channel

Ouabain-Induced Changes in the Expression of Voltage-Gated Potassium Channels in Epithelial Cells Depend on Cell–Cell Contacts

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