Ion Mobility Spectrometry

Kirkwood, Kaylie I; Odenkirk, Melanie T.; Baker, Erin

doi:10.1002/9783527836512.ch6

Cited by 2 publications

(1 citation statement)

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“…A way to circumvent the problem of distinguishing endogenous compounds from isomers potentially formed by autoxidation is to introduce a separation step between surface-sampling and mass spectrometry analysis. One possibility is ion mobility separation, where after ionisation ions are separated based on their size, shape and charge (47), however, this has yet to show separation of sterol isomers. A different strategy is to decouple surface-sampling from MS analysis.…”

Section: Msi Of Sterols Exploiting Chemical Derivatisationmentioning

confidence: 99%

Mass Spectrometry Imaging of Cholesterol and Oxysterols

Griffiths,

Yutuc,

Wang

2023

Advances in Experimental Medicine and Biology

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Mass spectrometry imaging (MSI) is a new technique in the toolbox of the analytical biochemist. It allows the generation of a compound specific image from a tissue slice where a measure of compound abundance is given pixel by pixel, usually displayed on a colour scale. As mass spectra are recorded at each pixel the data can be interrogated to generate images of multiple different compounds all in the same experiment. Mass spectrometry (MS) requires ionisation of analytes but cholesterol and other neutral sterols tend to be poorly ionised by the techniques employed in most MSI experiments, so despite its high abundance in mammalian tissues cholesterol is poorly represented in the MSI literature. In this article we discuss some of the MSI studies where cholesterol has been imaged and introduce newer methods for its analysis by MSI. Disturbed cholesterol metabolism is linked to many disorders and the potential of MSI to study cholesterol, its precursors and its metabolites in animal models and from human biopsies will be discussed.

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Section: Msi Of Sterols Exploiting Chemical Derivatisationmentioning

confidence: 99%