Ion-pair RP-HPLC method for the estimation of eberconazole nitrate in bulk form and in pharmaceutical dosage forms

Krishna, Marothu Vamsi; Dash, Rajendra N.; Reddy, B. Jalachandra; Venugopal, Parameswaran; Sandeep, P.; Madhavi, G.

doi:10.1556/achrom.25.2013.3.7

Cited by 3 publications

(2 citation statements)

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“…Eberconazole Nitrate was estimated in bulk form and pharmaceutical dosage forms by M. Vamsi Krishna using Ion-Pair RP-HPLC Method. 75% of 10 mM potassium dihydrogen phosphate containing 10 mM tetra-butyl ammonium hydroxide (cationic IPR) and 25% of methanol were used as a mobile phase [ 15 ]. A high pH ion-pairing strategy was used in 2015 by J. R. Dentona for the chromatographic determination of 2-hydroxypyridine-1-oxide (HOPO) in pharmaceutically relevant materials using hydroxy benzotriazole (HOBt) as a coupling reagent (cationic IPR) [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Ionisable substances chromatography: A new approach for the determination of Ketoprofen, Etoricoxib, and Diclofenac sodium in pharmaceuticals using ion – pair HPLC

Andraws

Trefi

2020

Heliyon

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An ion-pair HPLC method was developed and validated to analyze three of non-steroidal anti-inflammatory drugs (Ketoprofen, Etoricoxib, and Diclofenac sodium) in their pure and pharmaceuticals based on their ionisable characteristics. Cetyltrimethylammonium bromide (Cetrimide) was used as an ion pair reagent since it had not been used before for this purpose. Chromatographic analysis was accomplished using the C18 (250 × 4.6 mm, 5μm) column. Mobile phase consisted of a mixture of 50% Cetrimide M and 50% acetonitrile to analyze Ketoprofen and Etoricoxib, whereas for Diclofenac sodium, mobile phase was a mixture of 30% Cetrimide M and 70% acetonitrile. pH value was adjusted if necessary to 10 with ammonium hydroxide. The flow rate was 1mL/min and detection wavelengths were at 254 nm, 234 nm, and 254 nm for Ketoprofen, Etoricoxib, and Diclofenac sodium; respectively under ambient temperature. Retention times ( ) were 9.41, 7.34, and 6.66 for Ketoprofen, Etoricoxib, and Diclofenac sodium; respectively. The proposed method was evaluated for linearity, accuracy, precision, and specificity according to ICH guidelines. Ketoprofen, Etoricoxib, and Diclofenac sodium were detected in the following linear ranges: (0.031–0.500mg/mL), (0.007–0.110g/mL), and (0.016–0.250mg/mL); respectively with excellent mean recovery values (98.0–102.0%). RSD% was in an acceptable range (less than 2), proving the precision of the developed method. Specificity was proved in the presence of degradation products. Furthermore, a comparison between the results of this study and the reported HPLC methods indicated that this developed method was better in terms of simplicity, analysis time, and no use of buffers in the mobile phase. In conclusion, the developed method can successfully detect Ketoprofen, Etoricoxib, and Diclofenac sodium quantitatively and qualitatively in their dosage forms without any interference with excipients, making this method valuable, reliable, and practical to be applied in quality control laboratories.

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Section: Introductionmentioning

confidence: 99%

Ionisable substances chromatography: A new approach for the determination of Ketoprofen, Etoricoxib, and Diclofenac sodium in pharmaceuticals using ion – pair HPLC

Andraws

Trefi

2020

Heliyon

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“…A detailed literature survey for methylparaben, mometasone furoate and eberconazole nitrate revealed that determination of individual compound or combination with other drugs have been reported by HPLC [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] , LCMS [17][18] , electrophoresis 19 and spectrophotometric method 20 .…”

mentioning

confidence: 99%

Development and Validation of a Novel Stability Indicating RP-UPLC Method for Simultaneous Estimation of Methylparaben, Mometasone Furoate and Eberconazole Nitrate in Combined Topical Pharmaceutical Dosage Form

2013

Chem Sci Trans

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A novel, rapid, simple, stability indicating RP-UPLC method development and validation for simultaneous estimation of methylparaben (MP), mometasone furoate (MF) and eberconazole nitrate (EN) in topical pharmaceutical dosage form. Isocratic chromatographic separation was achieved on Acquity BEH C18, column using mobile phase consist of buffer (0.1%v/v triethylamine in water pH 6.0 by Glacial acetic acid), acetonitrile in the ratio (40:60, v/v) at flow rate 0.4 mL/min and detection was monitored at 235 nm by using photo-diode array detector. The method shows excellent linearity over the range of 2.75-152.7 µg/mL, 4.45-60.7 µg/mL and 9.10-606.6 µg/mL for methylparaben, mometasone furoate and eberconazole nitrate respectively. Recovery was found for all the components to be in the range of 98.4-101.7%. Stability indicating capability of developed method was established by analysing forced degradation samples in which spectral purity of methylparaben, mometasone furoate and eberconazole nitrate along with separation of degradation products from analytes peak. The proposed method was successfully applied for the quantitative estimation of MP, MF and EN in cream sample.

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The direct electrochemistry and bioelectrocatalysis of nitrate reductase at a gold nanoparticles/aminated graphene sheets modified glassy carbon electrode

Zhang

Zhou

et al. 2019

RSC Adv.

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It is rather difficult to achieve the direct electrochemistry of nitrate reductase (NR) as it is a complex homodimeric enzyme. However, in this study, the direct electron transfer between NR's redox centers and the electrode surface was achieved with the aid of aminated graphene sheets (am-GSs) which could immobilize NR stably and control the orientation of the enzyme molecules on the surface of the modified electrode through electrostatic attractions. Moreover, when the gold nanoparticles (AuNPs) which could act as electronic wire were introduced to the modified electrode, the NR-based enzymatic reduction of nitrate was promoted and a sensitive electrochemical response regarding the electrochemical reduction of nitrate could be obtained at the NR/AuNPs/am-GSs/GC electrode. Under optimized conditions, a wide linear range from 1.0 Â 10 À6 mol L À1 to 2.0 Â 10 À3 mol L À1 was acquired with a low detection limit of 7 Â 10 À7 mol L À1 (S/N ¼ 3). The biosensor was successfully employed to determine the total nitrogen in environmental water samples and the results were in good accordance with those obtained by ultraviolet-visible spectrophotometry.As the isoelectric point of NR is 6.2, 31 if dissolving the enzyme in 0.1 mol L À1 PBS with pH value of 7.0, NR will be negatively 37208 | RSC Adv., 2019,9,[37207][37208][37209][37210][37211][37212][37213] This journal is

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Ion-pair RP-HPLC method for the estimation of eberconazole nitrate in bulk form and in pharmaceutical dosage forms

Cited by 3 publications

References 4 publications

Ionisable substances chromatography: A new approach for the determination of Ketoprofen, Etoricoxib, and Diclofenac sodium in pharmaceuticals using ion – pair HPLC

Ionisable substances chromatography: A new approach for the determination of Ketoprofen, Etoricoxib, and Diclofenac sodium in pharmaceuticals using ion – pair HPLC

Development and Validation of a Novel Stability Indicating RP-UPLC Method for Simultaneous Estimation of Methylparaben, Mometasone Furoate and Eberconazole Nitrate in Combined Topical Pharmaceutical Dosage Form

The direct electrochemistry and bioelectrocatalysis of nitrate reductase at a gold nanoparticles/aminated graphene sheets modified glassy carbon electrode

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