Ionic liquid-based dispersive liquid–liquid microextraction followed by dispersive solid phase extraction coupled with HPLC-DAD for the determination of sulfonylurea herbicides in soymilk samples

Li, Jilong; Xu, Weili; Wang, Xiujuan; Wu, Fengze; Wang, Lin; Feng, Jie; Wang, Zhibing; Zhang, Hanqi

doi:10.1080/10826076.2022.2058960

Cited by 3 publications

(1 citation statement)

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“…Recently, ionic liquid (IL) as a novel and eco-friendly type of solvent with good thermal stability and high solubility to different compounds has been exploited in extraction pretreatment for HPLC determination [ 11 , 12 , 13 , 14 ]. Additionally, an IL aqueous solution may be a good choice for two kinds of active constituent extraction [ 15 , 16 , 17 ]. Previously, HPLC-DAD [ 18 , 19 , 20 , 21 ], HPLC-ELSD [ 22 , 23 , 24 ], or HPLC-MS [ 25 , 26 , 27 ] have been established for the quantitative determination of some characteristic steroidal saponins or homoisoflavonoids in ZOJ or COJ.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Extraction and Determination of Characteristic Steroidal Saponins and Homoisoflavonoids in Zhejiang Ophiopogon japonicus

et al. 2022

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Zhejiang Ophiopogonjaponicus (ZOJ) is a specific variety of Ophiopogon japonicus with characteristic steroidal saponins and homoisoflavonoids, which are also main pharmacodynamic constituents with clinical effects, including curing inflammation and cardiovascular diseases. However, few analysis methods were applied to simultaneously and quantitatively determine two kinds of its constituents, and hazardous organic solvents are mostly used for extraction. In this study, a new validated simultaneous extraction and determination method for four characteristic steroidal saponins and homoisoflavonoids in ZOJ was established by ionic liquid–ultrasonic extraction (IL-UAE) combined with HPLC-DAD-ELSD analysis, which can be used for the quality control of ZOJ. Chromatographic separation was performed with a DAD wavelength at 296 nm, and the ELSD parameters of the drift tube temperature (DTT), atomizer temperature (AT), and nitrogen gas pressure (NGP) were set at 20% heating power, 70 °C, and 25 psi, respectively. The optimal IL-UAE conditions were 1 mol/L [Bmim]CF3SO3 aqueous solution, a liquid–material ratio of 40 mL/g, and an ultrasonic time of 60 min. The proposed method is reliable, reproducible, and accurate, which were verified with real sample assays. Consequently, this work will be helpful for the quality control of ZOJ. It can also present a promising reference for the simultaneous extraction and determination of different kinds of constituents in other medicinal plants.

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