Ionizing radiation impacts photochemical quantum yield and oxygen evolution activity of Photosystem II in photosynthetic microorganisms

Rea, Giuseppina; Esposito, Dania; Damasso, M.; Serafini, Agnese; Margonelli, Andrea; Faraloni, Cecilia; Torzillo, Giuseppe; Zanini, A.; Bertalan, Ivo; Johanningmeier, Udo; Giardi, Maria Teresa

doi:10.1080/09553000802460149

Cited by 28 publications

(22 citation statements)

References 38 publications

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“…Therefore, the difference between single beam ground-based irradiation (g and neutron with a maximum LET of 250 keV mm À1 ) and the complex mixture of particles over a wide range of energy inside the ISS could account for discrepancies that need to be taken into account when comparing ground simulation with space flight data. Rea et al (2008) recently underlined the difficulty to measure and above all, to reproduce different radiation components at the same time over a wide energy range.…”

Section: Discussionmentioning

confidence: 99%

Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

et al. 2009

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In view of long-haul space exploration missions, the European Space Agency initiated the MicroEcological Life Support System Alternative (MELiSSA) project targeting the total recycling of organic waste produced by the astronauts into oxygen, water and food using a loop of bacterial and higher plant bioreactors. In that purpose, the a-proteobacterium, Rhodospirillum rubrum S1H, was sent twice to the International Space Station and was analyzed post-flight using a newly developed R. rubrum whole genome oligonucleotide microarray and high throughput gel-free proteomics with Isotope-Coded Protein Label technology. Moreover, in an effort to identify a specific response of R. rubrum S1H to space flight, simulation of microgravity and space-ionizing radiation were performed on Earth under identical culture set-up and growth conditions as encountered during the actual space journeys. Transcriptomic and proteomic data were integrated and permitted to put forward the importance of medium composition and culture set-up on the response of the bacterium to space flight-related environmental conditions. In addition, we showed for the first time that a low dose of ionizing radiation (2 mGy) can induce a significant response at the transcriptomic level, although no change in cell viability and only a few significant differentially expressed proteins were observed. From the MELiSSA perspective, we could argue the effect of microgravity to be minimized, whereas R. rubrum S1H could be more sensitive to ionizing radiation during long-term space exploration mission.

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Section: Discussionmentioning

confidence: 99%

Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

et al. 2009

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“…According to Rea et al [9] the microalga C. reinhardtii as an example does not seem to be influenced negatively by increased radiation concerning O 2 evolution and yield of photosystem II (compare to Fig. 1).…”

Section: Potential Of Microalgae With Respect To Remote Applicationsmentioning

confidence: 94%

“…Environmental factors in space that organisms have to deal with are (i) microgravity, (ii) space radiation, (iii) hypoxia, and (iv) low atmospheric pressure, as well as (v) extreme temperatures [3,9]. Those extreme habitats basically share the same problems limiting the spreading of life: water, clean air, food, and energy supply [10].…”

Section: Potential Of Microalgae With Respect To Remote Applicationsmentioning

confidence: 99%

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Photobioreactors in Life Support Systems

Wagner

Braun

Slenzka³

et al. 2015

Microalgae Biotechnology

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Life support systems for long-term space missions or extraterrestrial installations have to fulfill major functions such as purification of water and regeneration of atmosphere as well as the generation of food and energy. For almost 60 years ideas for biological life support systems have been collected and various concepts have been developed and tested. Microalgae as photosynthetic organisms have played a major role in most of these concepts. This review deals with the potentials of using eukaryotic microalgae for life support systems and highlights special requirements and frame conditions for designing space photobioreactors especially regarding illumination and aeration. Mono- and dichromatic illumination based on LEDs is a promising alternative for conventional systems and preliminary results yielded higher photoconversion efficiencies (PCE) for dichromatic red/blue illumination than white illumination. Aeration for microgravity conditions should be realized in a bubble-free manner, for example, via membranes. Finally, a novel photobioreactor concept for space application is introduced being parameterized and tested with the microalga Chlamydomonas reinhardtii. This system has already been tested during two parabolic flight campaigns.

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“…Different approaches, such as space research and physical elicitations, have been applied to select microorganisms with improved tolerance to extreme environmental conditions. The newly selected organisms generated for biosensor purposes were able to maintain a stable photosynthetic efficiency and an increased oxygen evolution capacity (Rea et al, 2008). In particular, we carried out modifications of the D1 reaction centre proteins, as they play a crucial role in electron tunnelling-mediated charge separation and transmembranal electric field generation, acting principally on reduction, release and migrations of (plasto) quinones.…”

Section: Relevance Of Genetic Engineering To Improve Sensitivity and mentioning

confidence: 99%

Computational Biology, Protein Engineering, and Biosensor Technology: a Close Cooperation for Herbicides Monitoring

Rea¹,

Polticelli²,

Antonacci³

et al. 2011

Herbicides, Theory and Applications

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Ionizing radiation impacts photochemical quantum yield and oxygen evolution activity of Photosystem II in photosynthetic microorganisms

Abstract: A potential role of PSII in capturing and utilizing ionizing radiation energy is postulated.

Cited by 28 publications

References 38 publications

Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

Photobioreactors in Life Support Systems

Computational Biology, Protein Engineering, and Biosensor Technology: a Close Cooperation for Herbicides Monitoring

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