iPSC-derived cranial neural crest-like cells can replicate dental pulp tissue with the aid of angiogenic hydrogel

Kobayashi, Yoshifumi; Nouet, Julie; Baljinnyam, Erdenechimeg; Siddiqui, Zain; Fine, Daniel H.; Fraidenraich, Diego; Kumar, Vivek; Shimizu, Emi

doi:10.1016/j.bioactmat.2021.11.014

Cited by 13 publications

(11 citation statements)

References 81 publications

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“…A cell-laden microfiber based on GelMA is used to carry seed cells and has achieved a good regeneration effect [ 43 ]. Cranial neural crest-derived cells (CNCLCs) obtained from induced pluripotent stem cells (iPSCs) were attached to the hydrogel to form a pulp-like tissue, such as harboring odontoblast-like cells, a dentin-like layer, and vast neovascularization [ 44 ].…”

Section: Application Of Hydrogel In Pulp Tissue Regenerationmentioning

confidence: 99%

Research Advances on Hydrogel-Based Materials for Tissue Regeneration and Remineralization in Tooth

Zhang

Guo

2023

Gels

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Tissue regeneration and remineralization in teeth is a long-term and complex biological process, including the regeneration of pulp and periodontal tissue, and re-mineralization of dentin, cementum and enamel. Suitable materials are needed to provide cell scaffolds, drug carriers or mineralization in this environment. These materials need to regulate the unique odontogenesis process. Hydrogel-based materials are considered good scaffolds for pulp and periodontal tissue repair in the field of tissue engineering due to their inherent biocompatibility and biodegradability, slow release of drugs, simulation of extracellular matrix, and the ability to provide a mineralized template. The excellent properties of hydrogels make them particularly attractive in the research of tissue regeneration and remineralization in teeth. This paper introduces the latest progress of hydrogel-based materials in pulp and periodontal tissue regeneration and hard tissue mineralization and puts forward prospects for their future application. Overall, this review reveals the application of hydrogel-based materials in tissue regeneration and remineralization in teeth.

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Section: Application Of Hydrogel In Pulp Tissue Regenerationmentioning

confidence: 99%

Research Advances on Hydrogel-Based Materials for Tissue Regeneration and Remineralization in Tooth

Zhang

Guo

2023

Gels

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“…Besides dental stem cells, induced pluripotent stem cell (iPSC), which is obtained by introducing reprogramming factors including octamer-binding transcription factor 4 (Oct4), Kruppel-like factor 4 (Klf4), sex determining region Y-box 2 (Sox2), l-myc, c-myc, and Lin28 to somatic cells, can also be used in pulp-dentin regeneration. (62)(63)(64)(65) Stem cells, such as DPSCs (63), and differentiated cells, such as fibroblasts (64) could be used to generate iPSCs. Generation of odontoblasts-like cells could be performed by directly inducing iPSCs.…”

Section: Regenerative Endodonticsmentioning

confidence: 99%

“…(63) In addition, iPSCs could be induced toward iPSCs-derived neural crestlike cells (iNCLCs), which in turn can be differentiated further into odontoblasts-like cells. (63,64) Differentiation to odontoblasts and generation of pulp-like tissue from iPSCs can be induced by transfection of specific genes (62), as well as addition of exogenous growth factors (63,64) and scaffolds (64).…”

Section: Regenerative Endodonticsmentioning

confidence: 99%

Crucial Triad in Pulp-Dentin Complex Regeneration: Dental Stem Cells, Scaffolds, and Signaling Molecules

et al. 2023

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BACKGROUND: Pulp damage can lead to dentinogenesis impairment, irreversible pulpitis, or pulp necrosis. Despite being the most used endodontic procedure to treat damaged pulp, root canal therapy only results in nonvital teeth which are prone to fractures and secondary infection. Pulp-dentin regeneration has a potential to regenerate structure similar to normal pulp-dentin complex, and can be achieved by combining dental stem cells, scaffold, and signaling molecules. This article reviews the role of various types of dental stem cells, scaffolds, signaling molecules, and their combinations in regenerating pulp-dentin complex.CONTENT: Dental pulp stem cell (DPSC), stem cell from human exfoliated deciduous teeth (SHED), and dental follicle stem cell (DFSC) were reported to regenerate pulp-dentin complex in situ. SHED might be more promising than DPSCs and DFSCs for regenerating pulp-dentin complex, since SHED have a higher proliferation potential and higher expression levels of signaling molecules. Scaffolds have characteristics resembling extracellular matrix, thus providing a suitable microenvironment for transplanted dental stem cells. To accelerate the regeneration process, exogenous signaling molecules are often delivered together with dental stem cells. Scaffolds and signaling molecules have different regenerative potential, including induction of cell proliferation and migration, formation of pulp- and/or dentin-like tissue, as well as angiogenesis and neurogenesis promotion.SUMMARY: Combinations of dental stem cells, scaffold, and signaling molecules are important to achieve the functional pulp-dentin complex formation. Current trends and future directions on regenerative endodontics should be explored. The right combination of dental stem cells, scaffold, and signaling molecules could be determined based on the patients’ characteristics. Incomplete pulp-dentin regeneration could be overcome by applying dental stem cells, scaffold, and/or signaling molecules in multiple visits.KEYWORDS: pulp-dentin regeneration, regenerative endodontics, dental stem cells, scaffold, signaling molecules

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“…Dental pulp played an irreplaceable role in maintaining tooth health, and its regeneration was the main goal of regenerative pulp therapy. 144 BG was a bonebonding calcio silicophosphate glass-ceramic material. Ag-BG/ chitosan hydrogel was prepared and used for pulp restoration.…”

Section: Tissue Engineeringmentioning

confidence: 99%

A review of recent advances in metal ion hydrogels: mechanism, properties and their biological applications

et al. 2022

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iPSC-derived cranial neural crest-like cells can replicate dental pulp tissue with the aid of angiogenic hydrogel

Cited by 13 publications

References 81 publications

Research Advances on Hydrogel-Based Materials for Tissue Regeneration and Remineralization in Tooth

Research Advances on Hydrogel-Based Materials for Tissue Regeneration and Remineralization in Tooth

Crucial Triad in Pulp-Dentin Complex Regeneration: Dental Stem Cells, Scaffolds, and Signaling Molecules

A review of recent advances in metal ion hydrogels: mechanism, properties and their biological applications

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