iQuant Auto: Automated Rapid Test Platform for Immunodiagnostics

Kumarasami, Ramdayalan; Vasan, Jayaraman Kiruthi; Joseph, Jayaraj; Sithambaram, Prabhakar; Pandidurai, Sathish; Sivaprakasam, Mohanasankar

doi:10.1109/embc44109.2020.9176134

Cited by 1 publication

(2 citation statements)

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“…This experiment was designed to investigate the CRISPR-based dFnCas9 detection of the Leptospira lipL32 gene using the lateral flow immunoassay strip, which involved dispensing capture biomolecules onto the test lines of the NC membrane, the addition of Alexa-labelled biomolecules on to the conjugate pads, cartridge assembly, and running of samples containing with the adequate sample biomolecules. The typical test procedure involved the addition of 90 µL of the appropriate lateral flow chase buffer to the sample pad of the LFIA cartridge, which was subsequently inserted into the iQuant analyzer [ 21 ]. The process was monitored for about 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…Upon DNA loading, an automatic sandwiched assay was completed within 15 min, allowing for ultra-specific assay compared to other sensors enabling on-field analysis or bedside testing. The lateral flow paper strip was used on the quantitative strip reader iQuant developed at the Healthcare Technology Innovation Centre, Indian Institute of Technology, Madras, India [ 19 , 20 , 21 ]. The advantages of the reader and performance comparison with other systems can be found in the literature [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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A Lateral Flow Assay for the Detection of Leptospira lipL32 Gene Using CRISPR Technology

Natarajan

Joseph

Balamurugan

et al. 2023

Sensors

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The clinical manifestation of leptospirosis is often misdiagnosed as other febrile illnesses such as dengue. Therefore, there is an urgent need for a precise diagnostic tool at the field level to detect the pathogenic Leptospira lipL32 gene at the molecular level for prompt therapeutic decisions. Quantitative polymerase chain reaction (qPCR) is widely used as the primary diagnostic tool, but its applicability is limited by high equipment cost and the lack of availability in every hospital, especially in rural areas where leptospirosis mainly occurs. Here, we report the development of a CRISPR dFnCas9-based quantitative lateral flow immunoassay to detect the lipL32 gene. The developed assay showed superior performance regarding the lowest detectable limit of 1 fg/mL. The test is highly sensitive and selective, showing that leptospirosis diagnosis can be achieved with a low-cost lateral flow device.

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Section: Methodsmentioning

confidence: 99%