Iranian Trichogramma: ITS2 DNA characterization and natural Wolbachia infection

Poorjavad, Nafiseh; Goldansaz, Seyed Hossein; Machtelinckx, Thijs; Tirry, Luc; Stouthamer, Richard; Leeuwen, Thomas Van

doi:10.1007/s10526-011-9397-z

Cited by 31 publications

(31 citation statements)

References 60 publications

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“…Parthenogenesis inducing Wolbachia infecting Trichogramma species are exclusively found in the B supergroup (Pintureau et al, 2000;Poorjavad et al, 2012;Van Meer et al, 1999;Schulenburg et al, 2000;Werren et al, 1995b;Zhou et al, 1998). Several other Wolbachia infections in Trichogramma have been found that belong to the supergroup A, among others the infection in T. bourarachae and T. kaykai (LC110).…”

Section: Discussionmentioning

confidence: 99%

Phylogeny of the Trichogramma endosymbiont Wolbachia, an alpha-proteobacteria (Rickettsiae)

Almeida

Stouthamer²

2017

Braz. J. Biol.

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Wolbachia (Hertig) endosymbionts are extensively studied in a wide range of organisms and are known to be transmitted through the egg cytoplasm to the offsping. Wolbachia may cause several types of reproductive modifications in arthropods. In Trichogramma species, parthenogenesis-inducing Wolbachia bacteria allow females wasps to produce daughters from unfertilized eggs and these bacteria are present in at least 9% of all Trichogramma species. Phylogenetic studies have led to the subdivision of the Wolbachia clade in five supergroups (A, B, C, D and E) and Wolbachia from Trichogramma belong to supergroup B. Here, using the wsp gene, four groups of Wolbachia that infect Trichogramma species were distinguished and the addition of a new group "Ato" was suggested due to the addition of Wolbachia from Trichogramma atopovirilia (Oatman and Platner). Specific primers were designed and tested for the "Ato" group. Seventy-five percent of all evaluated Wolbachia strains from Trichogramma fell within "Sib" group.Keywords: symbiont, egg parasitoid, DNA sequencing, phylogenetic relationships. and Platner, 1983). Primers específicos foram construídos e testados para o grupo "Ato". Setenta e cinco por cento de todas as linhagens de Wolbachia que infectam Trichogramma se enquadraram dentro do grupo "Sib". Filogenia do endosimbiontePalavras-chave: simbionte, parasitoide de ovos, sequenciamento de DNA, filogenia.

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Section: Discussionmentioning

confidence: 99%

Phylogeny of the Trichogramma endosymbiont Wolbachia, an alpha-proteobacteria (Rickettsiae)

Almeida

Stouthamer²

2017

Braz. J. Biol.

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“…However, the small size of these parasitoids (<1 mm long) and lack of clear diagnostic features make then difficult to identify, and this confusion can impede the implementation of effective biological control programs (Smith & Hubbes, 1986;Pinto et al, 1989;Pinto & Stouthamer 1994). Given the difficulties in distinguishing species of the genera, Trichogramma and Telenomus, molecular studies have gained importance in recent years (Yuliarti et al, 2002;Sümer et al, 2009;Poorjavad et al, 2012;Nasir et al, 2013). Therefore, with the advent of molecular techniques, DNA-based approaches have been used to generate molecular markers that are useful for the characterization of closely related or cryptic species for biological control work (Landry et al, 1993;Hoy et al, 2000;Chang at al., 2001).…”

Section: Feza Can Cengiz 1 Introductionmentioning

confidence: 99%

First record of the egg parasitoids of Chilo partellus (Swinhoe, 1885) (Lepidoptera: Crambidae) in Turkey unveiled by DNA Barcoding

Can

Kaya

Ulaşli

et al. 2016

Turkish Journal of Entomology

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Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) is an invasive insect species attacking maize (Zea mays L.) and other cereal crops causing important yield losses. The occurrence of this insect in Turkey was first reported in maize growing areas of some provinces in the East Mediterranean region of Turkey in 2014. Chemical or other pest control methods do not always provide acceptable control of this pest, so biological control is considered an important alternative. However, for a successful biological control, the first step is to reliably identify the natural enemies of a target pest, which is difficult to achieve using methods based on morphology. Recent developments in molecular techniques allow more reliable identification of insect species and their parasitoids. Therefore, the aim of this study was to identify the egg parasitoids of C. partellus by molecular methods. Parasitized eggs were collected from maize fields in Hatay province, Turkey, from September to October 2014 and in September 2015. Eggs were maintained in the laboratory and emerging adult parasitoids were subjected to molecular analysis. Using DNA barcoding, two native natural enemies, Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae) and Telenomus busseolae (Gahan) (Hymenoptera: Platygastridae) were identified as egg parasitoids of C. partellus for the first time in Turkey.

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“…Trichogramma 속 ITS2 영역의 DNA 염기서열을 해독한 결과 종내 변이가 적 고 종간 염기서열 전체길이와 변이 차이가 커 종 구별을 위해 사 용될 수 있었다 Li et al, 2004). 그동안 많은 연구자들에 의해서 Trichogramma속 종들의 ITS2 전체 염기서열이 밝혀지고 GenBank 데이터베이스에 등록되고 있 으며 형태적 분류가 어려운 은폐종을 구별하여 왔다 (Amornsak et al, 1998;Silva et al, 1999;Stouthamer et al, 1999;Ciociola et al, 2001a, b;Li et al, 2004;Dang et al, 2005;Davies et al, 2006;Honda et al, 2006;Gariepy et al, 2007;Li, 2007;Kumar et al, 2009;Sumer et al, 2009;Jeong et al, 2010;Sumer et al, 2011;Karimi et al, 2012;Poorjavad et al, 2012;del Pino et al, 2013). 그러나 형태적으로 구별이 매우 힘든 T. minutum과 T.…”

Section: 방제하고자 하는 해충에 대해 효과가 좋은 기생벌 선발이 중요 한데 대량사육 과정에서 목적하지 않은 종이 섞unclassified

“…이중 옥수수에 가장 큰 문제 해충인 조명 나방의 알기생벌로는 수원지역에서 쌀좀알벌 (Lee et al, 1980;Jung et al, 2005)과 경남지역(진주, 하동, 밀양)에서 송충알벌 (Kim and Kim, 1991 Pinto and Stouthamer, 1994;Heimpel and de Boer, 2008;Poorjavad et al, 2012).…”

Section: 한국곤충명집(The Entomological Society Of Korea Andunclassified

Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis

Seo¹,

Jung²,

Park³

et al. 2014

Korean journal of applied entomology

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To identify the species of Trichogramma occurring in the corn fields of Korea as egg parasitoids of Ostrinia furnacalis, we sequenced the full-length of ITS2 nuclear rDNA from 112 parasitoids collected during this study. As a reference to distinguish species, we also retrieved full-length ITS2 sequences of 60 Trichogramma species from the NCBI GenBank database. On the basis of the size and 3′terminal sequence pattern of the ITS2 sequences, the Trichogramma samples collected in this study were divided into three groups (K-1, -2, and -3). Evolutionary distances (d) within and between groups based on ITS2 sequences were estimated to be ≤0.005 and ≥0.080, respectively. In the net average distance between groups or species, the d value between K-1 and T. ostriniae, K-2 and T. dendrolimi, and K-3 and T. confusum was the lowest, with values of 0.016, 0.001, and 0.002, respectively. In the phylogenetic tree, K-1 and K-2 were clustered with T. ostriniae and T. dendrolimi, respectively. However, K-3 was clustered with three different species, namely, T. confusum, T. chilonis, and T. bilingensis. NCBI BLAST results revealed that parasitoids belonging to K-1 and K-2 showed 99% identity with T. ostriniae and T. dendrolimi, respectively. Parasitoids in K-3 collected from Hongcheon showed 99-100% identity with T. confusum and T. chilonis, and one parasitoid in K-3 collected from Gochang had 98% identity with T. bilingensis, T. confusum, and T. chilonis. On the basis of these results, we infer that the species of Trichogramma collected in this study are closely related to T. ostriniae (K-1) and T. dendrolimi (K-2). However, it was not possible to distinguish species of K-3 using the ITS2 sequence alone.

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Iranian Trichogramma: ITS2 DNA characterization and natural Wolbachia infection

Cited by 31 publications

References 60 publications

Phylogeny of the Trichogramma endosymbiont Wolbachia, an alpha-proteobacteria (Rickettsiae)

Phylogeny of the Trichogramma endosymbiont Wolbachia, an alpha-proteobacteria (Rickettsiae)

First record of the egg parasitoids of Chilo partellus (Swinhoe, 1885) (Lepidoptera: Crambidae) in Turkey unveiled by DNA Barcoding

Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis

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