Iron oxide nanoparticle-containing microbubble composites as contrast agents for MR and ultrasound dual-modality imaging

Liu, Zhe; Lammers, Twan; Ehling, Josef; Fokong, Stanley; Bornemann, Jörg; Gätjens, Jessica

doi:10.1016/j.biomaterials.2011.05.019

Cited by 145 publications

(125 citation statements)

References 38 publications

Supporting

Mentioning

119

Contrasting

Order By: Relevance

“…28 The spectrum for BSA/BCNU NPs displayed additional peaks corresponding to the ν of C=O (1,726 cm ) from C-C-Cl. 23 The X-ray photoelectron spectroscopy also confirmed the results of FTIR analysis. The BSA NP sample showed C1s, N1s, and O1s peaks at 284 eV, 400 eV, and 532 eV, respectively.…”

Section: Results and Discussion Preparation And Characterization Of Bsupporting

confidence: 65%

“…19,20 It has been reported that multimolecular imaging modalities provide complementary information for concurrent imaging applications and hence have become an attractive area of investigation (ie, magnetic resonance [MR]/fluorescence imaging, photoinduced electron transfer/fluorescence, MR/ultrasound imaging, and photoinduced electron transfer/CT). [21][22][23][24][25] Among the many imaging techniques, MR imaging has been accredited to be one of the most powerful and noninvasive diagnostic devices for humans. However, obtaining detailed biological information on a subcellular level is difficult owing to limited resolution and low sensitivity of MR imaging.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

1,3-Bis(2-chloroethyl)-1-nitrosourea-loaded bovine serum albumin nanoparticles with dual magnetic resonance–fluorescence imaging for tracking of chemotherapeutic agents

Wei

Lin

Huang

et al. 2016

IJN

View full text Add to dashboard Cite

To date, knowing how to identify the location of chemotherapeutic agents in the human body after injection is still a challenge. Therefore, it is urgent to develop a drug delivery system with molecular imaging tracking ability to accurately understand the distribution, location, and concentration of a drug in living organisms. In this study, we developed bovine serum albumin (BSA)-based nanoparticles (NPs) with dual magnetic resonance (MR) and fluorescence imaging modalities (fluorescein isothiocyanate [FITC]-BSA-Gd/1,3-bis(2-chloroethyl)-1-nitrosourea [BCNU] NPs) to deliver BCNU for inhibition of brain tumor cells (MBR 261-2). These BSA-based NPs are water dispersible, stable, and biocompatible as confirmed by XTT cell viability assay. In vitro phantoms and in vivo MR and fluorescence imaging experiments show that the developed FITC-BSA-Gd/BCNU NPs enable dual MR and fluorescence imaging for monitoring cellular uptake and distribution in tumors. The T1 relaxivity (R1) of FITC-BSA-Gd/BCNU NPs was 3.25 mM −1 s −1 , which was similar to that of the commercial T1 contrast agent (R1 =3.36 mM −1 s −1 ). The results indicate that this multifunctional drug delivery system has potential bioimaging tracking of chemotherapeutic agents ability in vitro and in vivo for cancer therapy.

show abstract

Section: Results and Discussion Preparation And Characterization Of Bsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

1,3-Bis(2-chloroethyl)-1-nitrosourea-loaded bovine serum albumin nanoparticles with dual magnetic resonance–fluorescence imaging for tracking of chemotherapeutic agents

Wei

Lin

Huang

et al. 2016

IJN

View full text Add to dashboard Cite

show abstract

“…The novel NP-MBs also provide possibilities for image-guided delivery of NPs using both contrast enhanced ultrasound imaging and MRI [13,42], and the use of MRI to trace the NPs in various tissues. In addition, the NPs can be further targeted to specific biomarkers, allowing for active targeting and hence higher NP/drug accumulation at the disease site.…”

Section: Clinical Application Of the Np-mbsmentioning

confidence: 99%

“…A common approach for avoiding clearance by the immune system is coating the NPs with polyethylene glycol (PEG). Another important feature of the NPs is the possibility to incorporate imaging agents such as fluorescent dyes and magnetic resonance imaging (MRI) or positron emission tomography/single photon emission computed tomography (PET/SPECT) contrast agents, achieving a combination of therapeutics and diagnostics, so called "theranostics" [13,14].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle‐stabilized microbubbles for multimodal imaging and drug delivery

Mørch

Hansen

Berg

et al. 2015

Contrast Media & Molecular

View full text Add to dashboard Cite

Microbubbles (MBs) are routinely used as contrast agents for ultrasound imaging. The use of ultrasound in combination with MBs has also attracted attention as a method to enhance drug delivery.We have developed a technology platform incorporating multiple functionalities, including imaging and therapy in a single system consisting of MBs stabilized by polyethylene glycol (PEG) coated polymeric nanoparticles (NPs). The NPs, containing lipophilic drugs and/or contrast agents, are composed of the widely used poly(butyl cyanoacrylate) (PBCA) polymer and prepared in a single step. MBs stabilized by these NPs are subsequently prepared by self-assembly of NPs at the MB air/liquid interface. Here we show that these MBs can act as contrast agents for conventional ultrasound imaging. Successful encapsulation of iron oxide NPs inside the PBCA NPs is demonstrated, potentially enabling the NPs/MBs to be used as magnetic resonance imaging (MRI) and/or molecular ultrasound imaging contrast agents. By precise tuning of the applied ultrasound pulse, the MBs burst and the NPs constituting the shell are released. This could result in increased local deposit of NPs into target tissue providing improved therapy and imaging contrast compared to freely distributed NPs.

show abstract

“…27 The therapeutic and diagnostic capabilities of these contrast agents are ongoing and show potential clinical prospects. 28 Most human spinal cord injuries result from fracture and dislocation of the spinal column.…”

Section: Discussionmentioning

confidence: 99%

Quantitative assessment of spinal cord perfusion by using contrast-enhanced ultrasound in a porcine model with acute spinal cord contusion

et al. 2012

View full text Add to dashboard Cite

Objectives: To quantify spinal cord perfusion by using contrast-enhanced ultrasound (CEUS) in a porcine model with acute spinal cord injury. Methods: Microcirculatory changes of acute spinal cord injury were shown by CEUS in a porcine model with spinal cord contusion at three selected time points, coupling with conventional ultrasound (US) and Color Doppler US (CDFI). Time-intensity curves and perfusion parameters were also obtained by autotracking contrast quantification (ACQ) software in the epicenter of contusion site, adjacent region and distant region, respectively. Neurologic and histologic examinations were used to confirm the severity of injury. Results: Conventional US revealed the spinal cord was hypoechoic and homogeneous, whereas the dura mater, pia mater and cerebral aqueduct were hyperechoic. On CDFI intramedullary blood vessels were displayed as segmental and columnar. It was homogeneous on CEUS. After spinal cord contusion, the injured region on gray scale US was hyperechoic. CDFI demonstrated intramedullary blood vessels of adjacent region had increased and dilated during the observation period. On CEUS the epicenter of contusion site was hypoperfusion, whereas its adjacent region was hyperperfusion compared with the distant region. Quantitative analysis showed that peak intensity decreased in epicenters of contusion but increased in adjacent regions significantly at all time points (Po0.05). Evaluation of neurological function for post-contusion demonstrated significantly deterioration in comparison before injury (Po0.05). Conclusions: CEUS is a practical technique that provides overall views for evaluating microcirculatory pattern in spinal cord injury. Quantitative analysis shows the efficacy in assessment of perfusion changes after spinal cord injury.

show abstract

Iron oxide nanoparticle-containing microbubble composites as contrast agents for MR and ultrasound dual-modality imaging

Cited by 145 publications

References 38 publications

1,3-Bis(2-chloroethyl)-1-nitrosourea-loaded bovine serum albumin nanoparticles with dual magnetic resonance–fluorescence imaging for tracking of chemotherapeutic agents

1,3-Bis(2-chloroethyl)-1-nitrosourea-loaded bovine serum albumin nanoparticles with dual magnetic resonance–fluorescence imaging for tracking of chemotherapeutic agents

Nanoparticle‐stabilized microbubbles for multimodal imaging and drug delivery

Quantitative assessment of spinal cord perfusion by using contrast-enhanced ultrasound in a porcine model with acute spinal cord contusion

Contact Info

Product

Resources

About

Iron oxide nanoparticle-containing microbubble composites as contrast agents for MR and ultrasound dual-modality imaging

Cited by 145 publications

References 38 publications

1,3-Bis(2-chloroethyl)-1-nitrosourea-loaded bovine serum albumin nanoparticles with dual magnetic resonance&ndash;fluorescence imaging for tracking of chemotherapeutic agents

1,3-Bis(2-chloroethyl)-1-nitrosourea-loaded bovine serum albumin nanoparticles with dual magnetic resonance&ndash;fluorescence imaging for tracking of chemotherapeutic agents

Nanoparticle‐stabilized microbubbles for multimodal imaging and drug delivery

Quantitative assessment of spinal cord perfusion by using contrast-enhanced ultrasound in a porcine model with acute spinal cord contusion

Contact Info

Product

Resources

About

1,3-Bis(2-chloroethyl)-1-nitrosourea-loaded bovine serum albumin nanoparticles with dual magnetic resonance–fluorescence imaging for tracking of chemotherapeutic agents

1,3-Bis(2-chloroethyl)-1-nitrosourea-loaded bovine serum albumin nanoparticles with dual magnetic resonance–fluorescence imaging for tracking of chemotherapeutic agents