Iron protoporphyrin‐modified monolithic support for on‐line preconcentration of angiotensin prior to CE analysis

Yone, A.; Rusell, María Lucía; Grasselli, Mariano; Vizioli, Nora Matilde

doi:10.1002/elps.200600706

Cited by 14 publications

(15 citation statements)

References 9 publications

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“…Polymethacrylate monolithic discs and cartridges were applied in SPE of neutral, acidic, and basic compounds [231,232], pharmaceutical compounds [233 -235], proteins [236], and peptides [237]. A novel monolithic stir bar was prepared for sorption extraction [238].…”

Section: Sample Preparationmentioning

confidence: 99%

Polymethacrylate monolithic columns for capillary liquid chromatography

Urban

Jandera²

2008

J of Separation Science

121

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In recent years, continuous separation media have attracted considerable attention because of the advantages they offer over packed columns. This research resulted in two useful monolithic material types, the first based on modified silica gel and the second on organic polymers. This work attempts to review advances in the development, characterization, and applications of monolithic columns based on synthetic polymers in capillary chromatography, with the main focus on monolithic beds prepared from methacrylate-ester based monomers. The polymerization conditions used in the production of polymethacrylate monolithic capillary columns are surveyed, with attention being paid to the concentrations of monomers, porogen solvents, and polymerization initiators as the system variables used to control the porous and hydrodynamic properties of the monolithic media. The simplicity of their preparation as well as the possibilities of controlling of their porous properties and surface chemistries are the main benefits of the polymer monolithic capillary columns in comparison to capillary columns packed with particulate materials. The application areas considered in this review concern mainly separations in reversed-phase chromatography, ion-exchange chromatography, hydrophobic and hydrophilic interaction modes; enzyme immobilization and sample preparation in the capillary chromatography format are also addressed.

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Section: Sample Preparationmentioning

confidence: 99%

Polymethacrylate monolithic columns for capillary liquid chromatography

Urban

Jandera²

2008

J of Separation Science

121

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“…Especially, Fe-dP exhibited the highest affinity for the amino acid histidine. In a previous work, we fixed iron-protoporphyrin to a monolithic microcolumn for the selective online preconcentration of angiotensin I, previously to CE analysis [31]. This system demonstrated a high retention capacity with a remarkable increase in concentration sensitivity.…”

Section: The Stationary Phasementioning

confidence: 96%

Separation of peptides by open‐tubular capillary electrochromatography using Fe(III)‐deuteroporphyrin as a covalently attached stationary phase

et al. 2009

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The separation of seven biologically active peptides was attempted by open-tubular capillary electrochromatography in fused-silica capillaries chemically modified with iron (III)-deuteroporphyrin using UV-absorption detection at 214 nm. The effect of BGE pH and content of organic solvent modifier was investigated. The best separations were obtained in 25 mM phosphate (BGE), pH 4.0, containing 5% v/v ACN and 10 mM hydroquinone, which was added to prevent gas bubble formation. Considering the method sensitivity, lower concentration LODs were obtained for all peptides in their open-tubular capillary electrochromatography separation as compared with their CZE separation in bare fused-silica capillary. The iron (III)-deuteroporphyrin column proved to be highly stable over time and showed acceptable precision of migration times and corrected peak areas (RSD in the range 2-4%).

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“…The efficiency of phosphate buffer was previously reported [24,25,31]. For this reason, we proceed to optimize the system by varying the BGE pH (range 4.0-8.0), voltage, and injection times (not shown).…”

Section: Selection Of Running Conditionmentioning

confidence: 98%

Gold nanoparticle‐coated capillaries for protein and peptide analysis on open‐tubular capillary electrochromatography

2012

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We report a new method of immobilization of gold nanoparticles (AuNPs) on a fused-silica capillary through covalent binding. The resulting modified capillary was applied to electrophoretic systems to improve the efficiency of separation and the selectivity of selected solutes. The immobilization of AuNPs on the capillary wall was performed in a very simple and fast way without requiring heating. The surface features of an AuNP-coated capillary column were determined using the scanning electron microscopy. The chromatographic properties of AuNP-coated capillaries were investigated through variation of the buffer pH and separation voltage. Effective separations of synthetic peptides mixture were obtained on the AuNP-coated capillaries. The method shows a remarkable stability since it was reused about 900 times. The capacity factor was duplicated. Therefore, this modification is stable and can be applied to different separation purposes. A complex mixture of tryptic peptide fragments of HSA was analyzed in both the bare- and the AuNP-coated capillaries. Better electrophoretic peptide profile was observed when using the AuNP-coated capillary.

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Iron protoporphyrin‐modified monolithic support for on‐line preconcentration of angiotensin prior to CE analysis

Cited by 14 publications

References 9 publications

Polymethacrylate monolithic columns for capillary liquid chromatography

Polymethacrylate monolithic columns for capillary liquid chromatography

Separation of peptides by open‐tubular capillary electrochromatography using Fe(III)‐deuteroporphyrin as a covalently attached stationary phase

Gold nanoparticle‐coated capillaries for protein and peptide analysis on open‐tubular capillary electrochromatography

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