Iron uptake and bacteriocin genes among <i>Escherichia coli</i> strains from skin and soft‐tissue infections

Erjavec, Marjanca Starčič; Petkovšek, Živa; Predojević, Luka; Žgur-Bertok, Darja

doi:10.1111/apm.12663

Cited by 2 publications

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“…This result is consistent with previous studies that showed an association between the presence of common virulence genes and bacteriocin genes in UPEC [9,13]. Most of the virulence genes associated with bacteriocins in our study were iron uptake genes, supporting the view that the fyuA and iroN genes are associated with different bacteriocins [51]. In addition, we did not identify a positive association between the MDR profile and the presence of bacteriocin genes, which is in agreement with the results of a previous study [9].…”

Section: Discussionsupporting

confidence: 93%

Determining the Virulence Properties of Escherichia coli ST131 Containing Bacteriocin-Encoding Plasmids Using Short- and Long-Read Sequencing and Comparing Them with Those of Other E. coli Lineages

et al. 2019

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Escherichia coli ST131 is a clinical challenge due to its multidrug resistant profile and successful global spread. They are often associated with complicated infections, particularly urinary tract infections (UTIs). Bacteriocins play an important role to outcompete other microorganisms present in the human gut. Here, we characterized bacteriocin-encoding plasmids found in ST131 isolates of patients suffering from a UTI using both short- and long-read sequencing. Colicins Ia, Ib and E1, and microcin V, were identified among plasmids that also contained resistance and virulence genes. To investigate if the potential transmission range of the colicin E1 plasmid is influenced by the presence of a resistance gene, we constructed a strain containing a plasmid which had both the colicin E1 and blaCMY-2 genes. No difference in transmission range was found between transformant and wild-type strains. However, a statistically significantly difference was found in adhesion and invasion ability. Bacteriocin-producing isolates from both ST131 and non-ST131 lineages were able to inhibit the growth of other E. coli isolates, including other ST131. In summary, plasmids harboring bacteriocins give additional advantages for highly virulent and resistant ST131 isolates, improving the ability of these isolates to compete with other microbiota for a niche and thereby increasing the risk of infection.

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Section: Discussionsupporting

confidence: 93%

Determining the Virulence Properties of Escherichia coli ST131 Containing Bacteriocin-Encoding Plasmids Using Short- and Long-Read Sequencing and Comparing Them with Those of Other E. coli Lineages

et al. 2019

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Escherichia coli Affects Expression of Circadian Clock Genes in Human Hepatoma Cells

et al. 2021

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Recent research has indicated that dysbiosis of the gut microbiota can lead to an altered circadian clock of the mammalian host. Herein we developed an original system that allows real-time circadian studies of human HepG2 hepatoma cells co-cultured with bacteria. The HepG2 cells with stably integrated firefly luciferase reporter under the control of PERIOD2 promoter were co-cultured with E. coli strains isolated from human fecal samples from healthy individuals. The two E. coli strains differ in the phylogenetic group and the number of ExPEC virulence-associated genes: BJ17 has only two, and BJ23 has 15 of 23 tested. In the first 24 h, the E. coli BJ17 affected the HepG2 circadian clock more than BJ23. Cosinor analysis shows a statistically significant change in the amplitude of PER1 and 2 and the phase advance of PER3. A high percentage of necrotic and apoptotic cells occurred at 72 h, while a correlation between the number of ExPEC genes and the influence on the HepG2 core clock gene expression was observed. Our study reveals that the E. coli genetic background is important for the effect on the mammalian circadian clock genes, indicating possible future use of probiotic E. coli strains to influence the host circadian clock.

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Iron uptake and bacteriocin genes among Escherichia coli strains from skin and soft‐tissue infections

Abstract: Iron uptake and bacteriocin genes among Escherichia coli strains from skin and soft-tissue infections APMIS 125: 264-267

Cited by 2 publications

References 14 publications

Determining the Virulence Properties of Escherichia coli ST131 Containing Bacteriocin-Encoding Plasmids Using Short- and Long-Read Sequencing and Comparing Them with Those of Other E. coli Lineages

Determining the Virulence Properties of Escherichia coli ST131 Containing Bacteriocin-Encoding Plasmids Using Short- and Long-Read Sequencing and Comparing Them with Those of Other E. coli Lineages

Escherichia coli Affects Expression of Circadian Clock Genes in Human Hepatoma Cells

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