Is A High-fiber Diet able to Influence Ovalbumin-induced Allergic Airway Inflammation in a Mouse Model?

Abstract

Background:More recently, a large amount of experimental and clinical discovered that dietary- fiber intake would decrease the susceptibility to allergic airway disease (AAD) and respiratory inflammation.Objective:To investigate whether a fiber-intake supplement is able to influence the induction of AAD and to elucidate the interactive relationship.Methods:AAD model mice and control mice were raised on a fundamental diet with standard 4% fiber content, whereas other mice were fed a 10% fiber-content diet in the high fiber-content group, along with a 25% fiber-content diet instead in very-high fiber-content group. All experimental mice were sensitized and challenged with ovalbumin to induce allergic inflammation in both the upper and lower airways. Hallmarks of AAD were examined in terms of eosinophil infiltration and goblet cell metaplasia in subepithelial mucosa, T-helper type 1 (Th1) to Th2 skewing of the immune response. Furthermore, to elucidate the interrelations, we generated 16S ribosomal DNA from fecal samples and further validated the variation of colony composition in each group.Results:The excessive high-fiber supplement induced a promoting effect rather than a suppressive effect, including a rise in nasal rubbing and sneezing, an increase in eosinophil inflammation and goblet cell metaplasia in subepithelial mucosa, and promoted Th2 skewing of the immune response as well as the production of serum levels of ovalbumin-specific immunoglobulin E. Moreover, overconsumption of dietary fiber greatly altered the construction of bacterial flora in the intestinal tract, including an increased proportion of Firmicutes, Actinobacteria, and Proteobacteria, and a decreased proportion of Bacteroidetes.Conclusion:Our work indicated that, instead of a protecting impact, excessive fiber intake preformed a negative influence on the induction of AAD. Therefore, we suspected that an excessive supplement of dietary fiber might not be an advisable method for the prevention and treatment of AADs.

Role of Short Chain Fatty Acids and Apolipoproteins in the Regulation of Eosinophilia-Associated Diseases

“…In addition, several mouse models of allergic airway inflammation have highlighted the positive effects of high-fiber diet, pre-, and probiotics on the inflammatory response and airway hyperresponsiveness by changing the gut microbiota and elevating the levels of SCFAs [ 130 , 156 , 157 , 158 , 159 ]. Further, Trompette et al, showed that application of propionate, alone, mimicked the positive effect of the high-fiber diet and protected against house dust mite-induced allergic lung inflammation in a GPR41-dependent manner [ 160 ].…”