Is collagenase-3 (MMP-13) expression in chondrosarcoma of the jaws a true marker for tumor aggressiveness?

Zyada, Manal M.; Shamaa, Ali

doi:10.1186/1746-1596-3-26

Cited by 10 publications

(7 citation statements)

References 76 publications

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“…Conversely, C-20/A4 tested positive for MMP-3 unlike previously shown [Finger et al, 2004], with more modest levels of aggrecan compared to A13/BACii. The expression of MMP-3, MMP-9 and MMP-13 has been associated with chondrosarcomas [Kawashima et al, 1997;Soderstrom et al, 2001;Kuroki et al, 2002;Zyada and Shamaa, 2008], and correlates with their grading and capacity to metastasize to the lungs [Sakamoto et al, 1999;Hou et al, 2011], coincidentally, the main target for B[ ␣ ]P tumourgenesis in vivo. As some established chondrosarcoma cell lines including swarm rat chondrosarcoma [Breitkreutz et al, 1979], OUMS-27 [Kudo et al, 2007] and HCS-TG [Kudawara et al, 2004] produce col2, it may be suggested that A13/BACii is a B[ ␣ ]P-transformed chondrosarcoma cell line.…”

Section: Discussionmentioning

confidence: 99%

The Phenotypic Characterization of A13/BACii, a Novel Bovine Chondrocytic Cell Line with Differentiation Potential

Qusous

Kaneva

Can

et al. 2012

Cells Tissues Organs

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In cartilage research bovine articular cartilage is used as an alternative to human tissue. However, animal material is subject to availability and primary cultures undergo senescence, limiting their use. Here we report the immortalization of primary bovine chondrocytes, which could be used as a surrogate for freshly isolated chondrocytes. Chondrocytes were isolated from cartilage explants and immortalized using 1.0 µg/ml benzo[alpha]pyrene. For 3-dimensional culture, chondrocytes were resuspended in 0.5% low-melt agarose at high density (HD) and cultured for 24 h prior to determining changes in expression profile and morphology. A13/BACii chondrocytes acquired a ‘flat’ irregular morphology and a foetal-like cell volume (1,509.59 ± 182.04 µm³). The human cell line C-20/A4 showed a statistically similar volume and length to A13/BACii. Two-dimensional-cultured A13/BACii expressed elevated levels of type I collagen (col1), reduced levels of type II collagen (col2) compared to freshly isolated chondrocytes and an overall col2 to col1 expression ratio (col2:col1) of 0.11 ± 0.01. Upon 3-dimensional encapsulation, there was a significant rise in col2 expression in both A13/BACii and C-20/A4, suggesting a capacity for redifferentiation in both cell lines with a return of col2:col1 values of A13/BACii to values previously observed in primary chondrocytes. A13/BACii chondrocytes expressed aggrecan, matrix metalloproteinase (MMP)-3, MMP-9 and MMP-13, further supporting indications of the differentiated phenotype. Here we report the creation of a novel chondrocytic cell line and demonstrate its strong potential for redifferentiation upon HD 3-dimensional encapsulation, providing an alternative to conventional dedifferentiated cell lines and primary culture.

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Section: Discussionmentioning

confidence: 99%

The Phenotypic Characterization of A13/BACii, a Novel Bovine Chondrocytic Cell Line with Differentiation Potential

Qusous

Kaneva

Can

et al. 2012

Cells Tissues Organs

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“…MMP-13 expression has been implicated in both physiological and pathological conditions. It has been shown to be essential for normal generation of granulation tissue in mice (20), and it is expressed in various cancers (39)(40)(41)(42). MMP-13 plays predominant roles in rheumatoid arthritis and osteoarthritis, and increased expression of MMP-13 induces osteoarthritis in mice (43).…”

Section: Discussionmentioning

confidence: 99%

ANKRD1 Acts as a Transcriptional Repressor of MMP13 via the AP-1 Site

et al. 2014

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“…Chondrosarcoma is a type of bone cancer originating from cartilaginous tissue and is the second most common bone tumor (1). Chondrosarcomas respond poorly to radiotherapy and chemotherapy, which are the currently used treatment strategies for this malignancy, making the management of chondrosarcomas a challenge (2).…”

Section: Introductionmentioning

confidence: 99%

Resveratrol attenuates matrix metalloproteinase-9 and -2- regulated differentiation of HTB94 chondrosarcoma cells through the p38 kinase and JNK pathways

Gweon

Kim

2014

Oncology Reports

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Abstract.Resveratrol has been shown to possess anticancer, anti-aging, and anti-inflammatory properties. Matrix metalloproteinases (MMPs) appear to be responsible for much of the extracellular matrix (ECM) degradation observed in the progression of cancer, aging and inflammation. We found that resveratrol significantly inhibited MMP-2 and MMP-9, and induced the expression of type II collagen and sex-determining region Y-box (SOX)-9 and the production of sulfated proteoglycans in HTB94 chondrosarcoma cells. Moreover, inhibition of MMPs with an MMP inhibitor further enhanced the effects of resveratrol. Phosphorylation of p38 was increased and phosphorylation of c-Jun N-terminal kinase (JNK) was inhibited by resveratrol. Treatment with SB203580, a p38 kinase inhibitor, enhanced the suppression of MMP-2 and MMP-9 by resveratrol and inhibited resveratrol-induced stimulation of type II collagen and SOX-9 expression and production of sulfated proteoglycans. Treatment with SP600125, a JNK inhibitor, attenuated the effects of resveratrol on MMP-2 and MMP-9, and accelerated resveratrol-induced effects on type II collagen, SOX-9 and sulfated proteoglycan production. Our results suggest that resveratrol inhibits MMP-induced differentiation via the p38 kinase and JNK pathways in HTB94 chondrosarcoma cells.

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Is collagenase-3 (MMP-13) expression in chondrosarcoma of the jaws a true marker for tumor aggressiveness?

Cited by 10 publications

References 76 publications

The Phenotypic Characterization of A13/BACii, a Novel Bovine Chondrocytic Cell Line with Differentiation Potential

The Phenotypic Characterization of A13/BACii, a Novel Bovine Chondrocytic Cell Line with Differentiation Potential

ANKRD1 Acts as a Transcriptional Repressor of MMP13 via the AP-1 Site

Resveratrol attenuates matrix metalloproteinase-9 and -2- regulated differentiation of HTB94 chondrosarcoma cells through the p38 kinase and JNK pathways

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