“…The optimum temperature for nitrification is dependent on the site of isolation of the organism (64) but in the temperate regions it is approximately 25 C. Nitrification may be observed (13,40,42) over a wide range of pH [5][6][7][8][9][10][11][12][13], but the actual range with an isolated organism seems to be dependent on the pH of the soil from which it was isolated. Nonbiological nitrification has also been discussed (19,50,63).…”