Is the lipolytic response in porcine adipose tissue slices equivalent to the lipolytic response in isolated adipocytes?

Mersmann, Harry J.; Shparber, Alla

doi:10.1016/0020-711x(93)90527-l

Cited by 5 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NES PCATS exhibited good viability over at least 63 h in culture, which is in accordance with the viability reported for precision-cut slices of other tissues (Thiele et al, 2017). Most of the publications on adipose tissue slices report a good viability up to 24 h of culture (Mersmann and Shparber, 1993;Ramsay and Rosebrough, 2005;Cai et al, 2011). Only two of them cultured thin adipose tissue slices for longer periods, i.e., five (Anayama et al, 2015) and 14 days (Schopow et al, 2020).…”

Section: Discussionsupporting

confidence: 85%

In vitro Lipolysis and Leptin Production of Elephant Seal Blubber Using Precision-Cut Adipose Tissue Slices

et al. 2020

View full text Add to dashboard Cite

Adipose tissue plays key roles in energy homeostasis. Understanding its metabolism and regulation is essential to predict the impact of environmental changes on wildlife health, especially in fasting-adapted species. However, in vivo experimental work in wild vertebrates can be challenging. We have developed a novel in vitro approach of precision-cut adipose tissue slices from northern elephant seal (Mirounga angustirostris) as a complementary approach to whole animal models. Blubber biopsies were collected from 14 pups during early and late post-weaning fast (Año Nuevo, CA, United States), precision-cut into 1 mm thick slices and maintained in culture at 37°C for at least 63 h. The slices exhibited an efficient response to ß-adrenergic stimulation, even after 2 days of culture, revealing good in vitro tissue function. The response to lipolytic stimulus did not vary between regions of outer and inner blubber, but was higher at early than at late fast for inner blubber slices. At early fast, lipolysis significantly reduced leptin production. At this stage, inner blubber slices were also more efficient at producing leptin than outer blubber slices, especially in the non-lipolytic condition. This model will aid the study of adipose tissue metabolism and its response to environmental stressors in marine mammals.

show abstract

Section: Discussionsupporting

confidence: 85%

In vitro Lipolysis and Leptin Production of Elephant Seal Blubber Using Precision-Cut Adipose Tissue Slices

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Such studies were valid from the scientific standpoint for demonstrating a biological event, but could not be directly extrapolated to a complex organism due to the large number of interactions with other metabolic pathways (López-Carlos et al 2011). Mersmann and Shparber (1993) found isolated adipocytes to be smaller than in vivo adipocytes, but the quantification of lipolysis was similar. Thus, there were innumerous exceptions to this rule.…”

Section: Discussionmentioning

confidence: 99%

Effect of ractopamine on lipid metabolism in vivo - a systematic review

Ferreira

Garbossa

Oberlender

et al. 2013

Braz. arch. biol. technol.

View full text Add to dashboard Cite

The aim of this article was to evaluate the in vivo mechanism of action through which ractopamine reduces the amount of adipose tissue in carcass of animals fed diets supplemented with this drug. Literature search was carried out, using the keywords Ractopamine, lipogenesis, lipolysis, fatty acid and adipose tissue. The scoring system was designed for the adequacy of the methodology. A total of eight papers were found-all published in the English language. The determination of lipolysis and lipogenesis was carried out using different methods-from the quantification of non-esterified fatty acids in the blood to molecular analyses of adipose tissue. The review analysis suggested that, at least in the pigs, the use of this drug reduced lipid deposition in the carcass due to a greater inhibition of lipogenesis than an increase in lipolysis.

show abstract

Cyclic AMP metabolism by swine adipocyte microsomal and plasma membranes

Zacher

Carey

1999

Comparative Biochemistry and Physiology Part B: Biochemistry an

View full text Add to dashboard Cite

Is the lipolytic response in porcine adipose tissue slices equivalent to the lipolytic response in isolated adipocytes?

Cited by 5 publications

References 19 publications

In vitro Lipolysis and Leptin Production of Elephant Seal Blubber Using Precision-Cut Adipose Tissue Slices

In vitro Lipolysis and Leptin Production of Elephant Seal Blubber Using Precision-Cut Adipose Tissue Slices

Effect of ractopamine on lipid metabolism in vivo - a systematic review

Cyclic AMP metabolism by swine adipocyte microsomal and plasma membranes

Contact Info

Product

Resources

About