Ischemia/Reperfusion Injury Promotes and Granulocyte-Colony Stimulating Factor Inhibits Migration of Bone Marrow-Derived Stem Cells to Endometrium

Du, Hongling; Naqvi, Hanyia; Taylor, Hugh S.

doi:10.1089/scd.2011.0193

Cited by 114 publications

(120 citation statements)

References 35 publications

(39 reference statements)

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“…Distinct from the few prior murine in vivo studies which support the concept of the bone marrow as a source of endometrial cells [9,[12][13][14][15], the present studies are the first to evaluate engraftment of the endometrium with bone marrow-derived cells at multiple time points post bone marrow transplant. We followed recipient animals for as long as 12 months post transplant to assess for long term engraftment of the uterus with bone marrow-derived cells.…”

Section: Discussionmentioning

confidence: 98%

“…The laboratory of Taylor and colleagues [12] identified Y chromosome-positive endometrial epithelial and stromal cells in female recipients of BMT from male donors six months post-transplant. These investigators subsequently demonstrated in a male-to-female murine BMT model that migration of BMDCs to the endometrium is promoted by ischemic uterine injury [15], and inhibited by exposure to cigarette smoke [14]. A male-to-female murine transplant model was also used by the laboratory of Mints and colleagues, who identified endothelial cells of donor origin in recipient endometria 40 days post-transplant [9], but did not evaluate whether other endometrial cell types were donor-derived.…”

Section: Discussionmentioning

confidence: 99%

“…Although recent in vivo data from human [8][9][10][11] and murine [9,[12][13][14][15] model systems support the concept that the bone marrow is an important contributor of stem cells to the endometrium, these prior studies have several limitations. Human studies which have demonstrated bone marrow-derived endometrial cells in bone marrow transplant recipients are severely limited in the number of subjects studied.…”

Section: Introductionmentioning

confidence: 99%

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Experimental Evidence for Bone Marrow as a Source of Nonhematopoietic Endometrial Stromal and Epithelial Compartment Cells in a Murine Model1

Morelli¹,

Rameshwar

Goldsmith³

2013

Biology of Reproduction

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Human endometrium has the remarkable ability to regenerate all cellular compartments with every menstrual cycle; the cellular source remains unknown. The objective of the present study was to determine whether the bone marrow (BM) is a source of multiple endometrial cell types using a murine BM transplant model. BM cells were harvested from transgenic donor mice that ubiquitously express green fluorescent protein (GFP) and were injected into lethally irradiated, syngeneic female recipient mice. Recipients with successful hematopoietic reconstitution were sacrificed at 3, 5, 9, and 12 mo posttransplant, after which hysterectomy was performed. Numbers of GFP-positive, CD45-positive, and CD45-negative cells in the endometrial stromal and epithelial compartments were determined. In the stromal compartment, BM-derived cells (BMDCs) were detectable as early as 3 mo posttransplant, and the BM remained a long-term contributor of nonhematopoietic endometrial cells. Nonhematopoietic endometrial cells comprised 47.3%-72.2% of total BMDCs in the stromal compartment at 12 mo posttransplant. In contrast, BMDCs were not detected in the glandular or luminal epithelial compartments until 12 mo posttransplant. These data demonstrate that the BM is a significant source of nonhematopoietic endometrial stromal compartment cells and contributes to a much lesser extent to the epithelial compartments. That BM is a source of nonhematopoietic cells in the endometrial stromal and epithelial compartments provides a potential mechanism by which monthly regeneration of the endometrium may occur.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Experimental Evidence for Bone Marrow as a Source of Nonhematopoietic Endometrial Stromal and Epithelial Compartment Cells in a Murine Model1

Morelli¹,

Rameshwar

Goldsmith³

2013

Biology of Reproduction

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“…Bone marrow-derived stem cells can transdifferentiate into endometrial epithelial and stromal cells [67]. They also contribute toward the regeneration of the endometrium after ischemia/reperfusion injury [68].…”

Section: Environmental Niches and Lrscmentioning

confidence: 99%

Label-Retaining Stromal Cells in Mouse Endometrium Awaken for Expansion and Repair After Parturition

Cao

Chan

Yeung

2015

Stem Cells and Development

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Human and mouse endometrium undergo dramatic cellular reorganization during pregnancy and postpartum. Somatic stem cells maintain homeostasis of the tissue by providing a cell reservoir for regeneration. We hypothesized that endometrial cells with quiescent properties (stem/progenitor cells) were involved in the regeneration of the endometrial tissue. Given that stem cells divide infrequently, they can retain the DNA synthesis label [bromodeoxyuridine (BrdU)] after a prolonged chase period. In this study, prepubertal mice were pulsed with BrdU and after a 6-week chase a small population of label-retaining stromal cells (LRSC) was located primarily beneath the luminal epithelium, adjacent to blood vessels, and near the endometrialmyometrial junction. Marker analyses suggested that they were of mesenchymal origin expressing CD44 + , CD90+ , CD140b + , CD146 + , and Sca-1 + . During pregnancy, nonproliferating LRSC predominately resided at the interimplantation/placental loci of the gestational endometrium. Immediately after parturition, a significant portion of the LRSC underwent proliferation (BrdU + /Ki-67 + ) and expressed total and active b-catenin. The b-catenin expression in the LRSC was transiently elevated at postpartum day (PPD) 1. The proliferation of LRSC resulted in a significant decline in the proportion of LRSC in the postpartum uterus. The LRSC returned to dormancy at PPD7, and the percentage of LRSC remained stable thereafter until 11 weeks. This study demonstrated that LRSC can respond efficiently to physiological stimuli upon initiation of uterine involution and return to its quiescent state after postpartum repair.

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“…Ischemia reperfusion uterine injury one week after bone marrow transplantation increased bone marrow-derived cell (BMDC) recruitment to the uterine stroma by two-fold compared to that in non-injured animals [52]. Without ischemic injury, an average of 22 and 13 Y chromosome signals were detected per 100,000 cells in the stromal and epithelial compartments respectively.…”

Section: Stem Cells In Endometriummentioning

confidence: 99%

The Endometrium as a Source of Mesenchymal Stem Cells for Regenerative Medicine1

Mutlu

Hufnagel

Taylor

2015

Biology of Reproduction

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Stem cell therapies have opened new frontiers in medicine with the possibility of regenerating lost or damaged cells. Embryonic stem cells, induced pluripotent stem cells, hematopoietic stem cells, and mesenchymal stem cells have been used to derive mature cell types for tissue regeneration and repair. However, the endometrium has emerged as an attractive, novel source of adult stem cells that are easily accessed and demonstrate remarkable differentiation capacity. In this review, we summarize our current understanding of endometrial stem cells and their therapeutic potential in regenerative medicine.

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Ischemia/Reperfusion Injury Promotes and Granulocyte-Colony Stimulating Factor Inhibits Migration of Bone Marrow-Derived Stem Cells to Endometrium

Cited by 114 publications

References 35 publications

Experimental Evidence for Bone Marrow as a Source of Nonhematopoietic Endometrial Stromal and Epithelial Compartment Cells in a Murine Model1

Experimental Evidence for Bone Marrow as a Source of Nonhematopoietic Endometrial Stromal and Epithelial Compartment Cells in a Murine Model1

Label-Retaining Stromal Cells in Mouse Endometrium Awaken for Expansion and Repair After Parturition

The Endometrium as a Source of Mesenchymal Stem Cells for Regenerative Medicine1

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