ISFET Arrays in CMOS: A Head-to-Head Comparison Between Voltage and Current Mode

Miscourides, Nicholas; Georgiou, Pantelis

doi:10.1109/jsen.2018.2881499

Cited by 32 publications

(38 citation statements)

References 40 publications

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“…In addition, we show allelic discrimination using a pH sensitive variation of the proposed assay with a CMOS microchip (Fig. 5) 16 . The microchip consisted of an array of ISFET sensors which detected both alleles with a positive change in pH of around 1-1.48 pH units.…”

Section: Discussionmentioning

confidence: 99%

“…were tested using an ISFET-based Lab-on-Chip platform 16,18 with synthetic DNA harbouring either the WT or MT allele to prove the feasible translation of the lab-based assay at a point-of-care setting. The reaction conditions were modified such that pH changes could occur during DNA amplification.…”

Section: Adaptation Of Snv-specific Lamp On a Lab-on-chip Platform Tmentioning

confidence: 99%

“…1a. These properties of LAMP coupled with its higher amplification yield allows it to be utilised in tandem with standard microchip technology used for chemical sensing such as the Ion-Sensitive Field-Effect Transistors (ISFETs) 16 . ISFETs fabricated in unmodified CMOS technology have been already described to detect DNA targets via the pH change induced during positive amplification results [17][18][19][20][21][22][23] .…”

mentioning

confidence: 99%

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A novel hotspot specific isothermal amplification method for detection of the common PIK3CA p.H1047R breast cancer mutation

Kalofonou

Malpartida-Cardenas

Alexandrou

et al. 2020

Sci Rep

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Breast cancer (BC) is a common cancer in women worldwide. Despite advances in treatment, up to 30% of women eventually relapse and die of metastatic breast cancer. Liquid biopsy analysis of circulating cell-free DNA fragments in the patients' blood can monitor clonality and evolving mutations as a surrogate for tumour biopsy. Next generation sequencing platforms and digital droplet PCR can be used to profile circulating tumour DNA from liquid biopsies; however, they are expensive and time consuming for clinical use. Here, we report a novel strategy with proof-of-concept data that supports the usage of loop-mediated isothermal amplification (LAMP) to detect PIK3CA c.3140 A > G (H1047R), a prevalent BC missense mutation that is attributed to BC tumour growth. Allele-specific primers were designed and optimized to detect the p.H1047R variant following the USS-sbLAMP method. The assay was developed with synthetic DNA templates and validated with DNA from two breast cancer cell-lines and two patient tumour tissue samples through a qPCR instrument and finally piloted on an ISFET enabled microchip. This work sets a foundation for BC mutational profiling on a Lab-on-Chip device, to help the early detection of patient relapse and to monitor efficacy of systemic therapies for personalised cancer patient management.Breast cancer (BC) has a lifetime incidence risk of 12%, with an overall survival of more than 70% of reported cases when early detection is possible 1,2 . Although surgery is capable of removing the primary tumour, minimal residual disease (MRD)/micrometastases may persist resulting in eventual resistance to therapy and recurrence 3 . MRD can often persist even after adjuvant therapy and can grow and spread overtime, remaining undetectable through mammograms, MRI scans and current tumour marker blood tests, such as CA-15-3 and CA 27.29 antigen assays 4 . These current tumour marker blood tests work as a cost-effective method to measure disease progression but do not provide information about mutational changes and heterogeneity of the primary tumour or MRD. With the development of new, non-cross-resistant treatments for breast cancer, early detection of MRD/micrometastases and mutational profiling of circulating tumour DNA (ctDNA) provides an attractive, cost-effective alternative approach to the detection of early signs of relapse and treatment switching.Previous research has proven that circulating free DNA (cfDNA) contains DNA fragments from cancer cells (ctDNA) that are released in blood, showing somatic mutations that reflect the original cancer and evolved clonal subtypes 5 . In particular, recent efforts have established that hotspot mutations in PIK3CA, a gene mutated in 20-40% of metastatic BC, are also commonly observed in screen-detected stage-1 BCs 6 . The PI3K protein is involved in the AKT/mTOR pathway and, when deregulated, leads to tumour-cell growth 7 . Investigation has

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Section: Discussionmentioning

confidence: 99%

Section: Adaptation Of Snv-specific Lamp On a Lab-on-chip Platform Tmentioning

confidence: 99%

mentioning

confidence: 99%

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A novel hotspot specific isothermal amplification method for detection of the common PIK3CA p.H1047R breast cancer mutation

Kalofonou

Malpartida-Cardenas

Alexandrou

et al. 2020

Sci Rep

Self Cite

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“…The developed LoC platform leverages on ISFET-based detection to facilitate fully-electronic chemical sensing and direct electrochemical signal transduction [37]. Sensor fabrication in CMOS technology achieves sensor miniaturisation, low cost manufacturing, scalability and sensitivity which are necessary for a pointof-care implementation.…”

Section: Discussionmentioning

confidence: 99%

“…The intrinsic pH sensitivity of the Si 3 N 4 layer has been measured to be 18 mV /pH with the final sensitivity of V out to pH after capacitive attenuation measured to be S pH = dVout dpH = −9.23 mV /pH . A detailed description of the circuit design, instrumentation pipeline and method to derive the pH sensitivity is provided in Miscourides & Georgiou [37].…”

Section: Isfet Sensing Arraymentioning

confidence: 99%

Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform

Malpartida-Cardenas

Miscourides

Rodríguez-Manzano

et al. 2019

Preprint

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Early and accurate diagnosis of malaria and drug-resistance is essential to effective disease management. Available rapid malaria diagnostic tests present limitations in analytical sensitivity, drug-resistant testing and/or quantification. Conversely, diagnostic methods based on nucleic acid amplification stepped forwards owing to their high sensitivity, specificity and robustness. Nevertheless, these methods commonly rely on optical measurements and complex instrumentation which limit their applicability in resource-poor, point-of-care settings. This paper reports the specific, quantitative and fully-electronic detection of Plasmodium falciparum, the predominant malaria-causing parasite worldwide, using a Lab-on-Chip platform developed in-house. Furthermore, we demonstrate on-chip detection of C580Y, the most prevalent single-nucleotide polymorphism associated to artemisinin-resistant malaria. Real-time non-optical DNA sensing is facilitated using Ion-Sensitive Field-Effect Transistors, fabricated in unmodified complementary metal-oxide-semiconductor technology, coupled with loop-mediated isothermal amplification. This work holds significant potential for the development of a fully portable and quantitative malaria diagnostic that can be used as a rapid point-of-care test. P. vivax (Pv) P. malariae (Pm) P. ovale curtisi (Poc) P. ovale wallikeri (Pow) P. knowlesi (Pk) P. cynomolgy (Pc) P. falciparum (Pf) P. vivax (Pv) P. malariae (Pm) P. ovale curtisi (Poc) P. ovale wallikeri (Pow) P. knowlesi (Pk) P. cynomolgy (Pc) P. falciparum (Pf) B2 5' 3'

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Recent advances in ion‐sensitive field‐effect transistors for biosensing applications

Peng

Mao

et al. 2022

Electrochemical Science Adv

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Over the past decades, considerable development and improvement can be observed in the area of the ion‐sensitive field‐effect transistor (ISFET) for biosensing applications. The mature semiconductor industry provides a solid foundation for the commercialization of the ISFET‐based sensors and extensive research has been conducted to improve the performance of ISFET, with a special research focus on the materials, device structures, and readout topologies. In this review, the basic theories and mechanisms of ISFET are first introduced. Research on ISFET gate materials is reviewed, followed by a summary of typical gate structures and signal readout methods for the ISFET sensing system. After that, a variety of biosensing applications including ions, deoxyribonucleic acid, proteins, and microbes are presented. Finally, the prospects and challenges of the ISFET‐based biosensors are discussed.

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ISFET Arrays in CMOS: A Head-to-Head Comparison Between Voltage and Current Mode

Cited by 32 publications

References 40 publications

A novel hotspot specific isothermal amplification method for detection of the common PIK3CA p.H1047R breast cancer mutation

A novel hotspot specific isothermal amplification method for detection of the common PIK3CA p.H1047R breast cancer mutation

Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform

Recent advances in ion‐sensitive field‐effect transistors for biosensing applications

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