Isoenzyme Data on the Genetic Divergence and Allopolyploidy in the Section Genea of the Grass Genus Bromus (Poaceae)

Oja, Tõnu; Jaaska, Vello

doi:10.1111/j.1601-5223.1996.00249.x

Cited by 15 publications

(16 citation statements)

References 18 publications

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“…This result would suggest that the octoploid derived from hybridization between the hexaploid B. rigidus (as maternal genome donor) and the diploid B. sterilis , which agrees with the early hypotheses from morphology, experimental crosses ( Cugnac and Camus, 1931 ) and isozyme data ( Oja and Jaaska, 1996 ;Oja and Laarman, 2002 ). This result would suggest that the octoploid derived from hybridization between the hexaploid B. rigidus (as maternal genome donor) and the diploid B. sterilis , which agrees with the early hypotheses from morphology, experimental crosses ( Cugnac and Camus, 1931 ) and isozyme data ( Oja and Jaaska, 1996 ;Oja and Laarman, 2002 ).…”

Section: Comparison Of Data Sets and Monophyly Of Section Genea -supporting

confidence: 86%

Molecular phylogeny and reticulate origins of the polyploid Bromus species from section Genea (Poaceae)

Fortuné

Pourtau

Viron

et al. 2008

American J of Botany

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The origin of polyploid Bromus species of section Genea was investigated using molecular data. This group of annual species native from the Old-World is composed of three diploids, two tetraploids, one hexaploid, and one octoploid. Molecular cloning, sequencing, and phylogenetic analyses were performed on several accessions per species. We used the low copy nuclear gene Waxy, repeated rDNA spacers ITS1 and ITS2 and chloroplast spacers trnT-trnL and trnL-trnF. Our analyses revealed four different lineages involved in the parentage of the polyploids and confirmed their reticulate origin. Three of these lineages are closely related to the diploid species B. sterilis, B. tectorum, and B. fasciculatus. The fourth lineage could not be related to any diploid according to the available data. Our data gave insights on the origin of all the polyploids of section Genea, and chloroplast data allowed us to identify the maternal lineages. The Waxy gene was the most informative regarding origin of the polyploids. The Waxy copies duplicated by polyploidy appear selectively maintained in the polyploid species. No sequence heterogeneity was encountered in the ITS region, where concerted evolution seems to have occurred toward either maternal or paternal repeats. These results provide new information about the origin and molecular evolution of these polyploids and will allow a more accurate taxonomic treatment of the concerned species, based on their evolutionary history.

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Section: Comparison Of Data Sets and Monophyly Of Section Genea -supporting

confidence: 86%

Molecular phylogeny and reticulate origins of the polyploid Bromus species from section Genea (Poaceae)

Fortuné

Pourtau

Viron

et al. 2008

American J of Botany

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“…The use of different terms is beneficial to define more precisely the isozymes encoded by different types of genes in polyploids. These terms were used in our previous publications ( Oja and Jaaska 1996; Oja 1998), describing isozymes in diploid and allopolyploid brome species.…”

Section: Methodsmentioning

confidence: 99%

“…Aat‐2 , Aat‐3 . Genetic interpretation of zymograms is based on the available information about the subunit structure of the enzyme studied and following the principles described by Weeden and Wendel (1989) and by Wendel and Weeden (1989), taking into account the results of a previous study ( Oja and Jaaska 1996).…”

Section: Methodsmentioning

confidence: 99%

“…Isozyme electrophoresis has been used in numerous studies to identify the progenitors of polyploids ( Crawford 1989; Thompson and Lumaret 1992; Soltis and Soltis 1993; Allen and Eccleston 1998; Nilsson and Jorde 1998). Isozymes have also been successfully used ( Oja and Jaaska 1996; Oja 1998) for discovering the allopolyloid nature of the Genea polyploids and to identify their putative diploid progenitors. Our results indicated that diploid species B. sterilis and B. tectorum could be donors of one composite genome of polyploid species, but the donor of the second genome of polyploids remained unidentified.…”

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Bromus fasciculatus Presl - a third diploid progenitor of Bromus section Genea allopolyploids (Poaceae)

Oja

2002

Hereditas

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Allozyme variation of ten heterozymes of seven enzymes among five accessions of a rare diploid Bromus fasciculatus was analysed with the use of PAGE and compared with that for six other species of section Genea of the genus Bromus. Allozymes charasteristic for diploids B. tectorum and B. fasciculatus are combined in fixed heterozygous phenotypes of tetraploid B. rubens. Fixed heterozygous phenotypes of tetraploid B. madritensis combine one allozyme of B. fasciculatus with another of diploid B. sterilis at each of the loci studied. Of the three diploids studied, only B. sterilis fits well for a role of a genome donor for the polyploid B. diandrus-rigidus complex. Bromus fasciculatus thus appears to be a diploid ancestor for the two tetraploids of section Genea, B. rubens and B. madritensis.

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“…Isoenzimas foram utilizadas com sucesso na determinação da diversidade gené-tica e das relações filogenéticas em outras espécies de Bromus, como aquelas das secções Genea (Oja & Jaaska, 1996; e Bromus (Oja, 1998 (Oja, 1999). Não houve correlação entre os grupos de similaridade genética (Figura 1) com as distâncias geográficas (Tabela 5), ou seja, acessos com menores distâncias geográficas não ficaram agrupados e vice-versa.…”

Section: Iniciador Seqüênciaunclassified

Variabilidade genética em populações naturais de Bromus auleticus Trin. ex Nees (Poaceae) com base em isoenzimas e marcadores RAPD

et al. 2005

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RESUMO -Bromus auleticus é uma espécie perene, nativa do estado do Rio Grande do Sul, que tem demonstrado adaptabilidade, potencial para produção de forragem de qualidade e alta diversidade morfológica. Neste trabalho, foram analisados padrões de bandas de sistemas enzimáticos e de marcadores RAPD, com o objetivo de determinar a variabilidade genética em 16 acessos de Bromus auleticus do Rio Grande do Sul. A variabilidade foi avaliada pelo índice de similaridade de Jaccard, utilizando-se o método UPGMA nas análises de agrupamento. Os índices de similaridade variaram de 0,0 a 0, 50 com base em isoenzimas e de 0,15 a 0,71 com base em marcadores RAPD. Os dados foram eficientes para a formação de grupos, indicando a variabilidade genética dos acessos analisados. Entretanto, houve baixa relação entre os agrupamentos e os locais de coleta. A variabilidade genética dos acessos é importante sob o ponto de vista do melhoramento genético, permitindo a seleção futura dos genótipos a partir de seus respectivos desempenhos. A utilização simultânea de isoenzimas e RAPD foi eficiente na caracterização da diversidade genética dos acessos analisados.Palavras-chave: Bromus auleticus, isoenzimas, RAPD, variabilidade genética, forrageiras nativas Genetic Variability in Natural Populations of Bromus auleticus Trin. ex Nees (Poaceae) Using Isozymes and RAPD MarkersABSTRACT -Bromus auleticus is a perennial, native species of the state of Rio Grande do Sul, which has demonstrated adaptability, potential yield of good quality forage as well as high morphological diversity. This work has analyzed band patterns from enzymatic systems and RAPD markers, with the objective to access the genetic variability in 16 accessions of Bromus auleticus from Rio Grande do Sul. The variability was evaluated using Jaccard similarity index. The method of grouping based on the average (UPGMA) was used in cluster analyses. The similarity index ranged from 0.0 to 0.50 using isozumes and from 0.15 to 0.71 using RAPD markers. The data have been efficient for the formation of different groups, indicating the genetic variability of the accession analyzed. However, these groupings have little relationship with the respective collecting places. The genetic variability of the accessions is important to the genetic improvement, allowing future genotype selection based on their respective performances. The simultaneous utilization of isozymes and RAPD was efficient in characterizing the genetic diversity of the accessions evaluated.

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Isoenzyme Data on the Genetic Divergence and Allopolyploidy in the Section Genea of the Grass Genus Bromus (Poaceae)

Cited by 15 publications

References 18 publications

Molecular phylogeny and reticulate origins of the polyploid Bromus species from section Genea (Poaceae)

Molecular phylogeny and reticulate origins of the polyploid Bromus species from section Genea (Poaceae)

Bromus fasciculatus Presl - a third diploid progenitor of Bromus section Genea allopolyploids (Poaceae)

Variabilidade genética em populações naturais de Bromus auleticus Trin. ex Nees (Poaceae) com base em isoenzimas e marcadores RAPD

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