Isoflavone concentrations in soybeans suitable for growing in Europe

Abstract

Isoflavones are nutraceuticals with many different medical benefits found abundantly in soybean (Glycine max (L.) Merr.) seeds. The prerequisite of utilising this valuable source of bioactive compounds and creating quality stock for the pharmaceutical and functional food industries is the screening of available soybean germplasm for isoflavone content. The objectives of this research were to determine the isoflavone concentrations (total isoflavones, daidzein, genistein, glycitein) in 22 high-yielding soybean genotypes, to investigate their variability and explore the effect of different weather conditions on isoflavone phenotypes. Field trials were set up as a randomised complete block design with two replicates in three consecutive years (2010-2012) at the Agricultural Institute Osijek (Osijek, Croatia). Chosen genotypes belonged to 00-II maturity groups (MGs) suitable for growing in almost all European regions. Results showed the existence of genetic diversity among the tested plant material. The influence of genotype and year were both statistically significant. The divergence determined by the analysis of variance (ANOVA) and confirmed by the pair-wise similarity based on the Euclidean distance, confirmed that this set of genotypes was suitable for the use in future crossing 48

β‐Cyclodextrin coupled to graphene oxide supported on aminopropyl silica as a sorbent material for determination of isoflavones

“…Two of the four target isoflavones investigated were found in all analyzed samples (Figure 3). Daidzein and genistein are considered as the principal isoflavones of soy and soy products [33]. On the other hand, formononetin and biochanin A are of much lower abundance in soy products.…”

Improving Seed Quality of Soybean Suitable for Growing in Europe

“…Murphy et al [199] determined 160-370 mg 100 −1 g range for TI content in RIL population tested in 2-year, multilocation trials in Canada, while Adie et al [194] Although breeding of isoflavone content at the Agricultural Institute Osijek is at its beginnings, some shifts in the right direction have been made which can be seen from the higher average isoflavone contents in elite breeding lines than in standard cultivars of the same MG (Table 4). In Matoša Kočar et al [195] study, genistein was the most abundant isoflavone component, followed by daidzein and glycitein. The same order of abundance was determined in the research by Lozovaya et al [198] for two French and three US cultivars, whereas Sumardi et al [215] researched 34 black soybeans in Indonesia and found that daidzein content was higher in 31 genotypes.…”

“…The most studied isoflavones are genistein, daidzein and glycitein [191]. As a quantitative trait controlled by many minor genes [192], isoflavone content in soybean seed largely depends on the environment [185,[193][194][195], to the extent that even the smallest changes in the microclimate can cause significant changes in the isoflavone contents. For example, it is reported that lower temperatures during the seed fill period increase the isoflavone content, whereas environments which were warmer and drier resulted in lower isoflavone contents [196][197][198][199].…”

“…In Europe, Cvejić et al[208] found TI content in 20 F1 soybean progenies ranged from 156 to 366 mg 100 −1 g, while Bursać et al[214] determined the TI content range of 211 to 524 mg 100 g −1 for different seed coat-coloured offsprings derived from the single cross between commercial variety and germplasm collection genotype with black seed coat. Matoša Kočar et al[195] investigated 22 MG 00 to II soybean genotypes from the Agricultural Institute Osijek (Croatia) during 3 years (2010-2012) and determined average TI content varied from 124.06 to 286.2 mg 100 −1 g, while average daidzein, glycitein and genistein contents were 49.27 mg 100 −1 g, 20.90 mg 100 −1 g and 94.41 mg 100 − 1 g DM, respectively.…”

Soybean for Human Consumption and Animal Feed

“…The most studied isoflavones are genistein, daidzein and glycitein [191]. As a quantitative trait controlled by many minor genes [192], isoflavone content in soybean seed largely depends on the environment [185,[193][194][195], to the extent that even the smallest changes in the microclimate can cause significant changes in the isoflavone contents.…”