Isogenic Barley Lines for Reaction to Erysiphe graminis F. Sp. Hordei1

Moseman, J. G.

doi:10.2135/cropsci1972.0011183x001200050038x

Cited by 75 publications

(49 citation statements)

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“…16151, and C.I. 16155, all of which are nearly isogenic derivatives of cv Manchuria (Moseman, 1972). These two copies were designated Bln1-1 and Bln1-2.…”

Section: Characterization Of Bln1 Transcriptsmentioning

confidence: 99%

Blufensin1Negatively Impacts Basal Defense in Response to Barley Powdery Mildew

2008

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Plants have evolved complex regulatory mechanisms to control the defense response against microbial attack. Both temporal and spatial gene expression are tightly regulated in response to pathogen ingress, modulating both positive and negative control of defense. BLUFENSIN1 (BLN1), a small peptide belonging to a novel family of proteins in barley (Hordeum vulgare), is highly induced by attack from the obligate biotrophic fungus Blumeria graminis f. sp. hordei (Bgh), casual agent of powdery mildew disease. Computational interrogation of the Bln1 gene family determined that members reside solely in the BEP clade of the Poaceae family, specifically, barley, rice (Oryza sativa), and wheat (Triticum aestivum). Barley stripe mosaic virus-induced gene silencing of Bln1 enhanced plant resistance in compatible interactions, regardless of the presence or absence of functional Mla coiled-coil, nucleotide-binding site, Leu-rich repeat alleles, indicating that BLN1 can function in an R-gene-independent manner. Likewise, transient overexpression of Bln1 significantly increased accessibility toward virulent Bgh. Moreover, silencing in plants harboring the Mlo susceptibility factor decreased accessibility to Bgh, suggesting that BLN1 functions in parallel with or upstream of MLO to modulate penetration resistance. Collectively, these data suggest that the grass-specific Bln1 negatively impacts basal defense against Bgh.

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“…16151, and C.I. 16155, all of which are nearly isogenic derivatives of cv Manchuria (Moseman, 1972). These two copies were designated Bln1-1 and Bln1-2.…”

Section: Characterization Of Bln1 Transcriptsmentioning

confidence: 99%

Blufensin1Negatively Impacts Basal Defense in Response to Barley Powdery Mildew

2008

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“…16151 line was obtained by introgression of the Mla6 gene into the universal susceptible cv Manchuria (Moseman, 1972). Seeds of C.I.…”

Section: Isolation Of Fast Neutron-derived Mutant Bcd1mentioning

confidence: 99%

Transcript-Based Cloning ofRRP46, a Regulator of rRNA Processing andRGene–Independent Cell Death in Barley–Powdery Mildew Interactions

et al. 2009

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Programmed cell death (PCD) plays a pivotal role in plant development and defense. To investigate the interaction between PCD and R gene-mediated defense, we used the 22K Barley1 GeneChip to compare and contrast time-course expression profiles of Blumeria graminis f. sp hordei (Bgh) challenged barley (Hordeum vulgare) cultivar C.I. 16151 (harboring the Mla6 powdery mildew resistance allele) and its fast neutron-derived Bgh-induced tip cell death1 mutant, bcd1. Mixed linear model analysis identified genes associated with the cell death phenotype as opposed to R gene-mediated resistance. Onehundred fifty genes were found at the threshold P value < 0.0001 and a false discovery rate <0.6%. Of these, 124 were constitutively overexpressed in the bcd1 mutant. Gene Ontology and rice (Oryza sativa) alignment-based annotation indicated that 68 of the 124 overexpressed genes encode ribosomal proteins. A deletion harboring six genes on chromosome 5H cosegregates with bcd1-specified cell death and is associated with misprocessing of rRNAs but segregates independent of R gene-mediated resistance. Barley stripe mosaic virus-induced gene silencing of one of the six deleted genes, RRP46 (rRNA-processing protein 46), phenocopied bcd1-mediated tip cell death. These findings suggest that RRP46, a critical component of the exosome core, mediates RNA processing and degradation involved in cell death initiation as a result of attempted penetration by Bgh during the barley-powdery mildew interaction but is independent of gene-for-gene resistance.

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“…16151 and C.I. 16155 are congenic lines (94% similar) (Moseman, 1972), methylation status may not be correlated with a particular genetic background; more likely, it is inherited with specific alleles (Cocciolone et al, 2001). …”

Section: Dna Methylation Associated With the Mla Locusmentioning

confidence: 99%

Genome Dynamics and Evolution of theMla(Powdery Mildew) Resistance Locus in Barley[W]

2002

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Genes that confer defense against pathogens often are clustered in the genome and evolve via diverse mechanisms. To evaluate the organization and content of a major defense gene complex in cereals, we determined the complete sequence of a 261-kb BAC contig from barley cv Morex that spans the Mla (powdery mildew) resistance locus. Among the 32 predicted genes on this contig, 15 are associated with plant defense responses; 6 of these are associated with defense responses to powdery mildew disease but function in different signaling pathways. The Mla region is organized as three gene-rich islands separated by two nested complexes of transposable elements and a 45-kb gene-poor region. A heterochromatic-like region is positioned directly proximal to Mla and is composed of a gene-poor core with 17 families of diverse tandem repeats that overlap a hypermethylated, but transcriptionally active, gene-dense island. Paleontology analysis of long terminal repeat retrotransposons indicates that the present Mla region evolved over a period of Ͼ 7 million years through a variety of duplication, inversion, and transposon-insertion events. Sequence-based recombination estimates indicate that R genes positioned adjacent to nested long terminal repeat retrotransposons, such as Mla , do not favor recombination as a means of diversification. We present a model for the evolution of the Mla region that encompasses several emerging features of large cereal genomes.

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Isogenic Barley Lines for Reaction to Erysiphe graminis F. Sp. Hordei¹

Cited by 75 publications

References 0 publications

Blufensin1Negatively Impacts Basal Defense in Response to Barley Powdery Mildew

Blufensin1Negatively Impacts Basal Defense in Response to Barley Powdery Mildew

Transcript-Based Cloning ofRRP46, a Regulator of rRNA Processing andRGene–Independent Cell Death in Barley–Powdery Mildew Interactions

Genome Dynamics and Evolution of theMla(Powdery Mildew) Resistance Locus in Barley[W]

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