Isolation and amino acid sequence of a mating pheromone produced by mating type α cells of <i>Saccharomyces exiguus</i>

We reviewed our investigations on sexual behaviors and interactions including sexual cell agglutination and pheromone action mainly in non-conventional yeasts, Hansenula anomala, H. wingei, Pichia amethionina, P. heedi, P. opuntiae, Saccharomyces kluyveri, S. globsus, S. exiguus, Saccharomycodes ludwigii. The techniques and genetic models including the cassette model and alpha 1-alpha 2 hypothesis which had been developed largely in S. cerevisiae were applicable to these yeasts in principle. The sexual agglutination was distinctly species-specific while sex pheromones were cross-reactive beyond species' barriers. The successful induction of heterothallic strains from homothallic strains in S. exiguus by mutagenesis enabled to the subsequent biochemical and genetical analysis of sexual behavior in the yeast. The phylogenetic consideration on sex differentiation is also included.

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“…Culture filtrate of S. exiguus ( Purification procedure of a pheromone from Saccharomyces exiguus (SAKURAI et al 1986).…”

Section: Phylogenetic Aspect O J Saccharomyces Species Differentiatiomentioning

confidence: 99%

Sexual behavior and its pheromonal regulation in ascosporogenous yeasts

Yoshida

Hisatomi

Yanagishima

1989

J. Basic Microbiol.

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“…The α mating pheromones of S. naganishii and S. cerevisiae are both comprised of 13 amino acids, but the sequences differ significantly (Sakurai et al , 1986). However, these α mating pheromones exhibit interspecies activity despite the infertility between the two species (Hisatomi et al , 1988).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and DNA analysis of a gene encoding α mating pheromone from the yeast Saccharomyces naganishii

Kodama¹,

Hisatomi²,

Kanemura³

et al. 2002

Yeast

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A DNA fragment encoding the precursor peptide for α mating pheromone was isolated from the S. naganishii genome based on the amino acid sequence of the mature pheromone. The precursor peptide contains three copies of the pheromone. Hydrophobicity analysis of the precursor peptide revealed an N-terminal signal sequence for translocation into the lumen of the endoplasmic reticulum and several signals for a series of secretion-related processes. However, upstream regulatory sequences necessary for expression of the S. cerevisiae α mating pheromone gene were not found, suggesting the divergence of systems that regulate α mating pheromone gene expression in S. naganishii and S. cerevisiae. Hybridization of a probe corresponding to the S. naganishii α mating pheromone nucleotide sequence to S. naganishii chromosomal DNA revealed a single gene located on either chromosome VI or VII. The S. naganishii α mating pheromone sequence has been deposited in the DDBJ/EMBL/GenBank data library under Accession No. AB086431.

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“…Most of these sequences concern nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA). The rest concern two autonomously replicating sequences, and only four nuclear genes encoding the orotidine 5′‐phosphate decarboxylase [8], E‐Ste3p, a homologue of the S. cerevisiae α‐receptor gene [9], the homologue of Abp1p, an actin‐binding protein [10], and the mating factor α [11]. In contrast to yeasts of the sensu stricto group, S. exiguus is a petite positive moderately inducible by ethidium bromide.…”

Section: Introductionmentioning

confidence: 99%

Genomic Exploration of the Hemiascomycetous Yeasts: 6. Saccharomyces exiguus

Bon¹,

Neuvéglise²,

Lépingle³

et al. 2000

FEBS Letters

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Random sequence tags were obtained from a genomic DNA library of Saccharomyces exiguus. The mitochondrial genome appeared to be at least 25.7 kb in size, with a different organization compared to Saccharomyces cerevisiae. An unusual putative 953 bp long terminal repeated element associated to Ty3 was found. A set of 1451 genes was identified homologous to S. cerevisiae open reading frames. Only five genes were identified outside the S. cerevisiae taxon, confirming that S. exiguus is phylogenetically closely related to S. cerevisiae. Unexpectedly, numerous duplicated genes were found whereas they are unique in S. cerevisiae. The sequences are deposited at EMBL under the accession numbers: AL407377^AL409955. ß

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Isolation and amino acid sequence of a mating pheromone produced by mating type α cells of Saccharomyces exiguus

Cited by 12 publications

References 8 publications

Sexual behavior and its pheromonal regulation in ascosporogenous yeasts

Sexual behavior and its pheromonal regulation in ascosporogenous yeasts

Molecular cloning and DNA analysis of a gene encoding α mating pheromone from the yeast Saccharomyces naganishii

Genomic Exploration of the Hemiascomycetous Yeasts: 6. Saccharomyces exiguus

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