Isolation and Analysis of Salt Response of Lactobacillus plantarum FS5-5 from Dajiang

Abstract

From 15 samples of dajiang collected in northeast of China, three salt resistant lactic acid bacteria were isolated and identified as Lactobacillus plantarum through physiological studies and 16S rDNA sequence alignment. L. plantarum FS5-5 showed better growth in an environment with 12 % (w/v) NaCl than the other two strains. The expression of proteins extracted from L. plantarum FS5-5 cultured in de Man, Rogosa, and Sharp (MRS) containing 0, 3, 6 and 9 % (w/v) NaCl was analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The results showed that 42 kinds of proteins were identified, which could be divided into three groups: 27 kinds of proteins related to protein synthesis and degradation, six kinds of proteins related to carbohydrate metabolism and energy metabolism, nine proteins related to nucleic acid metabolism. Overexpression of these proteins imply that a series of changes have occurred in the process of protein synthesis and degradation, carbohydrate metabolism, energy metabolism and nucleic acid metabolism after L. plantarum FS5-5 exposed to salt stress. All these proteins may have effects on the salt-tolerant characteristics of the L. plantarum FS5-5.

Probiotic capacity of commensal lactic acid bacteria from the intestine of Guinea pigs (Cavia porcellus)

“…Survival of LAB at different NaCl concentrations was evaluated as previously described [ 22 ], with slight modifications. Each of the LAB was reactivated in tubes with MRS medium at 37 °C for 24 h. Six tubes were prepared using 9 mL of MRS broth.…”