Isolation and antibiogram of Staphylococcus, Streptococcus and Escherichia coli isolates from clinical and subclinical cases of bovine mastitis

Mohanty, Nihar Nalini; Das, Paromita; Pany, Shaswati Subhadarsini; Sarangi, Laxmi Narayan; Ranabijuli, Siddharth; Panda, H. K.

doi:10.14202/vetworld.2013.739-743

Cited by 29 publications

(34 citation statements)

References 8 publications

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“…In this study, a total of 300 raw milk samples were studied and from these 300 raw milk samples, 75 (25%) milk samples were contaminated with E. coli. This is in agreement with the report by Mohanty et al, 15 who found (21%) from India. The current finding was relatively higher as compared to the studies by Lye et al, 16 & Addo et al, 17 who reported 8.75% and 11.2% from Malaysia and Ghana, respectively.…”

Section: Discussionsupporting

confidence: 94%

Isolation, identification and antimicrobial susceptibility testing of Escherichia coli isolated from selected dairy farms in and around mekelle, Ethiopia

Yohannes¹

2018

JDVAR

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A cross sectional study was conducted from November 2014 to April 2015 in selected dairy farms in and around Mekelle, Ethiopia with the objective of isolating and identifying Escherichia coli from raw cow's milk and determing its antimicrobial susceptibility pattern. From a total of 300 raw milk samples collected, 75 (25%) were found to be positive for E. coli organisms by culture method. Among the potential risk factors, stage of lactations (x 2 =149.677; P=0.000), age groups (x 2 =6.010; P=0.04) and parity numbers (x 2 =11.594; P=0.009) were found significantly associated with occurrence of E. coli. The highest percentage of E. coli isolates were found from cows' milk with early stage of lactation 53 (84.1%) and from cows with four and above parity number 43 (32.8%). On the other hand, the association between different farms were not statistically significant (x 2 =5.712; P=0.222.) with the occurrence of E. coli organisms. The antimicrobial susceptibility pattern revealed that all the isolates were found to be 100% susceptible to gentamicin followed by kanamycin (92%), sulphamethoxazole-trimthoprim (76%); were found to be 100% resistant to penicillin G followed by amoxicillin (84%) and tetracycline (60%). Therefore, gentamicin, kanamycin and sulphamethoxazole-trimethoprim were found to be the most effective drugs against E. coli. To safeguard the quality of raw milk, people engaged in milk and dairy production chain should be trained for hygienic practices.

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Section: Discussionsupporting

confidence: 94%

Isolation, identification and antimicrobial susceptibility testing of Escherichia coli isolated from selected dairy farms in and around mekelle, Ethiopia

Yohannes¹

2018

JDVAR

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“…In this study, a total of 300 raw milk samples were studied and from these 300 raw milk samples, 75 (25%) milk samples were contaminated with E. coli. This is in agreement with the report by Mohanty et al, 15 who found (21%) from India. The current finding was relatively higher as compared to the studies by Lye et al, 16 and Addo et al, 17 who reported 8.75% and 11.2% from Malaysia and Ghana, respectively.…”

Section: Discussionsupporting

confidence: 94%

Antimicrobial susceptibility testing of escherichia coli isolated from selected dairy farms in and around mekelle, Ethiopia

Yohannes¹

2018

JBMOA

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Abbreviations: CLSI, clinical and laboratory standards institute; CSA, central statistical agency; CVM, college of veterinary medicine; Lbs, libras; Ml, milliliter J Bacteriol Mycol Open Access. 2018;6(1):47-51. 47Abstract A cross sectional study was conducted from November 2014 to April 2015 in selected dairy farms in and around Mekelle, Ethiopia with the objective of determining the antimicrobial susceptibility pattern of Escherichia coli. From a total of 300 raw milk samples collected, 75 (25%) were found to be positive for Escherichia coli organisms by culture method. In this study, the antimicrobial susceptibility pattern revealed that all the isolates were found to be 100% susceptible to gentamicin followed by kanamycin (92%), sulphamethoxazoletrimthoprim (76%) on the other hand, in this study, all the isolates were found to be 100% resistant to penicillin G followed by amoxicillin (84%) and tetracycline (60%). Therefore, in this study gentamicin was the most effective drug against E. coli infection followed by kanamycin and sulphamethoxazole-trimethoprim. To safeguard the quality of raw milk, people engaged in milk and dairy production chain should be trained for hygienic practices.

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“…Statistically significant difference (P< 0.05) among the sample types in the prevalence of S. aureus was recorded. A similar report was also made by previous researchers (Abebe et al, 2013;Reta et al, 2016) that S. aureus was 15.5 and 24.2% in raw milk samples respectively in Ethiopia and in the other parts of the world which is contrary to this; different literatures revealed a very significant isolation rate of S. aureus from raw milk samples (Olatunji et al, 2009;Pourhassan and Taravat-Najafabadi, 2011;Mohanty et al, 2013;Sanaa et al, 2005).…”

Section: Discussionsupporting

confidence: 70%

Antimicrobial resistance profile of Staphylococcus aureus isolated from raw cow milk and fresh fruit juice in Mekelle, Tigray, Ethiopia

Abraha¹,

Hadish²,

Aligaz³

et al. 2018

J. Vet. Med. Anim. Health

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This study was conducted to evaluate drug resistance profile of Staphylococcus aureus in raw milk, fresh fruit juice and dairy farms settings of Mekelle, Tigray. A cross-sectional study was conducted on the total 258 samples of raw cow milk and fresh fruit juice. Antimicrobial resistance status was also checked for identified S. aureus using various commonly used antimicrobial discs. The overall viable staphylococcal count mean and standard deviation of samples from milk shop, fruit juice and dairy milk were found to be 8.86±107, 7.2 × 107, 8.65±107 cfu/ml, 33.87±106, 6.68±106 and 22.0±106, respectively. Among the total 258 samples, 75 (29.07%) samples were found positive for S. aureus. Proportion of the isolation from milk shop, fruit juice and dairy milk samples were 20 (23.26%), 32 (37.21%) and 23 (26.74%), respectively. Antimicrobial test of the high resistance revealed vancomycin (100%), ampicillin (90.9%). ciprofloxacin (90.9%), ceftaroline (63.6%), penicillin-G (81.8%) and clindamycin (72.7%) whereas they are highly susceptible to some antibiotics like gentamicin (100%), streptomycin (81.8%), norfloxacin (63.6%), chloramphenicol (81.8%), sulfamethoxazole (96%), kanamycin (72.7%), polymixin B (72.7%), erythromycin (72.7%) and tetracycline (81.8); also, some S. aureus also showed multi-drug resistance pattern. The present study, we isolated and determined the drug Resistance profile of S. aruesu in Mekelle city, Northern Ethiopia alarmingly, the S. aureus isolates circulating in the raw cow milk, fresh fruit juice and dairy milk. High level of S. aureus isolation from personnel and equipment besides food samples reveals that the hygiene practice is substandard. Prudent drug use and improved hygienic practice is recommended in the raw cow milk of dairy farms and fresh fruit juice to safeguard the public from the risk of acquiring infections and multiple drug resistance (MDR) pathogenic S. aureus.

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Isolation and antibiogram of Staphylococcus, Streptococcus and Escherichia coli isolates from clinical and subclinical cases of bovine mastitis

Cited by 29 publications

References 8 publications

Isolation, identification and antimicrobial susceptibility testing of Escherichia coli isolated from selected dairy farms in and around mekelle, Ethiopia

Isolation, identification and antimicrobial susceptibility testing of Escherichia coli isolated from selected dairy farms in and around mekelle, Ethiopia

Antimicrobial susceptibility testing of escherichia coli isolated from selected dairy farms in and around mekelle, Ethiopia

Antimicrobial resistance profile of Staphylococcus aureus isolated from raw cow milk and fresh fruit juice in Mekelle, Tigray, Ethiopia

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