Isolation and Celulolytic Activity Assay of Actinobacteria Isolated from Palm Oil Wastewater

Abstract

Cellulose plays an important role in the carbon cycle in nature and is the largest compound. This study aimed to isolate, to characterize and to determine actinobacteria that capable of producing cellulases. The sampling method in this study was carried out by purposive sampling at the outlet point of aeration pond of the wastewater treatment plant (IPAL) from palm oil waste station of PT. Teupin Lada. Isolation of actinobacteria was carried out on Humic Acid Vitamin b Agar (HVA), morphological characterization was carried out on Yeast Malt Agar (YMA), Yeast Starch Agar (YSA), Oatmeal Agar (OA), and microscopic characterization of actinobacteria and measuring the diameter of the clear zone formed on Carboxymethyl Cellulose (CMC) medium using the indicator Congo Red. Eight isolates were obtained from the isolation. Of the 8 isolates obtained, 7 of them were able to produce cellulase enzymes which were measured based on the clear zone formed in the test Congo Red on Carboxymethil Cellulose (CMC), and one isolate did not show any clear zones. The highest value of Cellulolytic Index (IS) was obtained from isolate ATLS-05, namely 8.38 mm. HIGHLIGHTS Cellulose plays an important role in the carbon cycle in nature and is the largest compound The high waste load especially palm oil mill effluent (Elaeis guineensis) or known as Palm Oil Mill Effluent(POME) could cause various problems for the environment and society. POME is wastewater from the palm oil industry, which is one of the most polluting agro-industrial wastes Actinobacteria are one of the soil microbes which have the greatest abundance and play an important role in the decomposition process. One of the roles of soil microbes is too degrading cellulose GRAPHICAL ABSTRACT