Isolation and characterization of 18 microsatellite loci for an endangered fresh water crocodile Tomistoma schlegelii

Chang, Yi Chun; Li, Shou Hsien; Chang, Ming Hsung; Chen, Szu Lung

doi:10.1007/s12686-013-0139-7

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…Members of the species reside in temporary freshwater pools formed by seasonal rains. Since these pools are geographically isolated from one another, molecular analysis tools used to examine promote better conservation practices [4,5]. In recent years, the development of microsatellite markers has been sped up using Next-Generation Sequencing technologies [e.g., 6,7].…”

Section: Introductionmentioning

confidence: 99%

“…This allows for subtle differences between populations to be observed using this type of marker. Additionally, microsatellites are useful in the conservation of endangered species, thus the development of markers can support the understanding of genetic diversity in endangered species to promote better conservation practices [ 4 , 5 ]. In recent years, the development of microsatellite markers has been sped up using Next-Generation Sequencing technologies [e.g., 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Development and characterization of microsatellite primers for Triops granarius (Branchiopoda: Notostraca) using MiSeq technology

et al. 2022

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Background Next-generation sequencing technology has allowed for the rapid development of microsatellites, neutral polymorphic markers that can be used for the analysis of population structure. Methods and Results In this study, we performed whole-genome sequencing using the Illumina MiSeq system and de novo assembly to design microsatellite primers for Triops granarius populations in Qatar. The developed microsatellites are suitable for future studies of genetic structuring among geographically isolated freshwater pools. A total of 23 different primer pairs produced typical microsatellite results, with each pair successfully amplified in up to 40 individuals. Only five of the loci produced a significant departure from Hardy-Weinberg equilibrium. Conclusions Some of the underlying mechanisms regarding the few loci that deviated from HWE may be further investigated to determine the source of deviation. As T. granarius is the most widely distributed species of the family, the development of these molecular markers would be useful for conducting population genetics and biogeographical studies broadly.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and characterization of microsatellite primers for Triops granarius (Branchiopoda: Notostraca) using MiSeq technology

et al. 2022

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The effectiveness of microsatellite DNA as a genetic tool in crocodilian conservation

Vashistha

Sharma

Dhakate

et al. 2020

Conservation Genet Resour

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Isolation and characterisation of 20 microsatellite loci from the Chinese green tree viper Trimeresurus stejnegeri stejnegeri

Chang

Lin²,

et al. 2016

Amphib Reptilia

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The Chinese green tree viper (Trimeresurus stejnegeri stejnegeri), one of the most common snakes in Southeast Asia, can be a good model species for evolutionary and behavioral research. However, there is no high polymorphic co-dominant marker that can be used for individual-based genetic analyses available for this species. Therefore, we developed 20 polymorphic microsatellite loci for T. s. stejnegeri in Taiwan by screening a microsatellite-enriched DNA library. The allele numbers of these loci ranged from 3 to 22, and the observed heterozygosity were 0.042-1.000. The probability of false parent non-exclusion was 2 × 10−5 for those loci that conformed to the Hardy–Weinberg equilibrium. These highly polymorphic microsatellite markers are useful for both individual-based and population-level analysis. Furthermore, this is the first set of microsatellite markers developed for Trimeresurus, and could be applied to closely related species to address various research questions on evolution and animal behavior.

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Isolation and characterization of 18 microsatellite loci for an endangered fresh water crocodile Tomistoma schlegelii

Cited by 3 publications

References 4 publications

Development and characterization of microsatellite primers for Triops granarius (Branchiopoda: Notostraca) using MiSeq technology

Development and characterization of microsatellite primers for Triops granarius (Branchiopoda: Notostraca) using MiSeq technology

The effectiveness of microsatellite DNA as a genetic tool in crocodilian conservation

Isolation and characterisation of 20 microsatellite loci from the Chinese green tree viper Trimeresurus stejnegeri stejnegeri

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