Isolation and characterization of a novel lepidopteran-selective toxin from the venom of South Indian red scorpion, Mesobuthus tamulus

Rajendra, Wudayagiri; Inceoglu, Bora; Herrmann, Robert O.; Derbel, Maher; Choudary, Prabhakara V; Hammock, Bruce D.

doi:10.1186/1471-2091-2-16

Cited by 46 publications

(11 citation statements)

References 37 publications

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“…These two peptides were present in both sources of venom. It is instructive, however, to examine the status of iberiotoxin,28 tamapin,25 and ButaIT29—three of the most well‐characterised peptides in Mesob. tamulus venom, none of which are represented in Table 2.…”

Section: Resultsmentioning

confidence: 99%

Mass fingerprinting of toxic fractions from the venom of the Indian red scorpion,Mesobuthus tamulus: biotope‐specific variation in the expression of venom peptides

Newton

Clench

Deshmukh

et al. 2007

Rapid Comm Mass Spectrometry

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The red scorpion, Mesobuthus tamulus, is found in two distinct biotopes within the Indian state of Maharastra-a tropical, sea-level biotope and a semi-arid biotope, up to 600 m. Scorpions from these two geographical areas show marked differences in toxicity. Using mass spectrometry, we have shown biotope-specific variation in the expression of peptides from scorpions collected from these two distinct areas. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) and reversed-phase liquid chromatography/electrospray ionisation mass spectrometry (LC/ESI-MS) were assessed as techniques for obtaining mass fingerprint data. On line LC/ ESI-MS was judged to be the method of choice and unique biotope-specific mass fingerprints, with key diagnostic markers, were obtained.

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Section: Resultsmentioning

confidence: 99%

Mass fingerprinting of toxic fractions from the venom of the Indian red scorpion,Mesobuthus tamulus: biotope‐specific variation in the expression of venom peptides

Newton

Clench

Deshmukh

et al. 2007

Rapid Comm Mass Spectrometry

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“…The percentage identity between the toxins and the net charge associated with them are shown on the right. The figure shows homology between the following toxins I5A, ITI1, I4, I5 and I3 from Buthus eupeus [31], AmmP2 from Androctonus mauretanicus [4,31], ClTx, ClTx‐a, b, c and d from Leiureus quinquestriatus quinquestriatus [4,13], Bs8 and Bs14 from Mesobuthus sindicus [32,33], LQH‐8/6 from L. quinquestriatus hebraeus [34], ButaIT from B. tamulus [24]. The pattern of disulfide bridges as determined by the structure of chlorotoxin is shown in this figure.…”

Section: Resultsmentioning

confidence: 99%

“…Tamulustoxin is another toxin isolated from B. tamulus venom, which is active on the K + channel [22]. Bt‐II, a mammalian‐specific toxin [23], and ButaIT, an insect‐specific toxin, are also isolated from B. tamulus [24].…”

Section: Introductionmentioning

confidence: 99%

“…Tamulustoxin is another toxin isolated from B. tamulus venom, which is active on the K þ channel [22]. Bt-II, a mammalian-speci¢c toxin [23], and ButaIT, an insect-speci¢c toxin, are also isolated from B. tamulus [24]. This paper describes the isolation, puri¢cation, and sequence determination of a short toxin, BtITx3, isolated from the Indian scorpion B. tamulus.…”

Section: Introductionmentioning

confidence: 99%

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Purification and characterization of a short insect toxin from the venom of the scorpion Buthus tamulus

et al. 2002

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A short chain peptide has been isolated from the venom of a red scorpion of Indian origin, Buthus tamulus. This peptide was puri¢ed using ion exchange and reverse phase chromatography and was characterized by molecular weight determination and amino acid sequence. The primary structure analysis shows that BtITx3 is a short peptide of 35 amino acid residues having a molecular weight of 3796 Da. The toxin shows toxicity towards the Lepidopteran species of insect Helicoverpa armigera causing £accid paralysis and even death within 24 h. It shows more than 50% homology with the short insectotoxins having four disul¢de bridges, which suggests that the toxin belongs to the class of short chain toxins blocking the chloride ion channels. This sequence homology study has also helped to bring out the structure^function relationship between the various short toxins. Homology modeling done by using template structure of a known toxin indicated that this toxin consists of a similar K K/L L sca¡old, as present in other scorpion toxins. ß 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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“…[7] BT venom contains serotonin, histamine, bradykinin potentiating factor, peptide toxins, etc., which are powerful nociceptive agents. [14][15][16] Thus, i.a. injection of venom stimulates vast population of nociceptors located around the peripheral blood vessels.…”

Section: Discussionmentioning

confidence: 99%

N-methyl-D-aspartate receptors mediate Mesobuthus tamulus venominduced vasosensory reflex responses in anesthetized rats

Singh¹,

Deshpande²

2017

Natl J Physiol Pharm Pharmacol

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Background:The autonomic changes and cardiorespiratory changes seen in vascular disorders are suggested to be mediated reflexly by the activation of perivascular nociceptors. The role of prostaglandins, vanilloid receptor 1, 5-hydroxytryptamine type 3, and kinin receptors is well established. Aims and Objectives: This study was conducted to understand the role of N-methyl-D-aspartate (NMDA) receptor in modulating vasosensory reflex responses evoked by Mesobuthus tumulus venom. Materials and Methods: Healthy male albino rats were anesthetized with an intra-peritoneal injection of urethane (1.5 g/kg). Tracheostomy was performed to keep the airway patent. Femoral artery was cannulated proximally as well as distally to record the blood pressure (BP) and to inject the chemicals, respectively. The effect of venom on BP, heart rate (HR), respiratory rate, and minute ventilation was recorded for 60 min at every 5 min and presented as mean ± standard error of mean. Results: Intra-arterial injection of venom produced immediate hyperventilatory response (increase of 41.6%), followed by hypoventilatory response (decrease of 40.1%), and finally sustained hyperventilatory response (increase of 53%) which was observed up to 60 min. The hypertensive response started at 40 s, peaking at 5 min (increase of 48%), and remained above the initial level subsequently. The bradycardiac response began around 5 min, peaking at 25 min (decrease of 50% in HR), and remained at that level up to 60 min. In 2-amino 5-phosphonovaleric acid (NMDA receptor antagonist) pre-treated group, the venom-induced cardiovascular responses (mean arterial pressure and HR) were markedly attenuated in comparisons to the venom only group but not the respiratory responses. Conclusions: The data provide evidence for the involvement of NMDA receptors in producing the venom-induced vasosensory reflex responses modulating the cardiovascular parameters in anesthetized rats.

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Isolation and characterization of a novel lepidopteran-selective toxin from the venom of South Indian red scorpion, Mesobuthus tamulus

Abstract: Background: Scorpion venom contains insect and mammal selective toxins. We investigated the venom of the South Indian red scorpion, Mesobuthus tamulus for the purpose of identifying potent insecticidal peptide toxins.

Cited by 46 publications

References 37 publications

Mass fingerprinting of toxic fractions from the venom of the Indian red scorpion,Mesobuthus tamulus: biotope‐specific variation in the expression of venom peptides

Mass fingerprinting of toxic fractions from the venom of the Indian red scorpion,Mesobuthus tamulus: biotope‐specific variation in the expression of venom peptides

Purification and characterization of a short insect toxin from the venom of the scorpion Buthus tamulus

N-methyl-D-aspartate receptors mediate Mesobuthus tamulus venominduced vasosensory reflex responses in anesthetized rats

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