Isolation and Characterization of a Novel Noncoding RNA from Nickel-Induced Lung Cancer

Zhang, Jing; Zhou, Yang; Wu, Youjun; Ma, Lin; Fan, Yingying; Kang, Xuan; Shi, Hongjun; Zhang, Jun

doi:10.1007/s12011-012-9460-3

Cited by 8 publications

(4 citation statements)

References 28 publications

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“…Nickel toxicities include skin allergies, lung fibrosis, and lung cancer (Zhao et al, 2009). A recent study has identified a novel 1280 bp non-coding RNA NRG1 associated with nickel-induced lung cancer and has suggested the potential role of lncRNAs in nickel carcinogenesis (Zhang et al, 2012).…”

Section: Lncrnas and Heavy Metalsmentioning

confidence: 99%

Long Non-Coding RNAs: A Novel Paradigm for Toxicology

Dempsey

Cui

2016

Toxicol. Sci.

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Long non-coding RNAs (lncRNAs) are over 200 nucleotides in length and are transcribed from the mammalian genome in a tissue-specific and developmentally regulated pattern. There is growing recognition that lncRNAs are novel biomarkers and/or key regulators of toxicological responses in humans and animal models. Lacking protein-coding capacity, the numerous types of lncRNAs possess a myriad of transcriptional regulatory functions that include cis and trans gene expression, transcription factor activity, chromatin remodeling, imprinting, and enhancer up-regulation. LncRNAs also influence mRNA processing, post-transcriptional regulation, and protein trafficking. Dysregulation of lncRNAs has been implicated in various human health outcomes such as various cancers, Alzheimer's disease, cardiovascular disease, autoimmune diseases, as well as intermediary metabolism such as glucose, lipid, and bile acid homeostasis. Interestingly, emerging evidence in the literature over the past five years has shown that lncRNA regulation is impacted by exposures to various chemicals such as polycyclic aromatic hydrocarbons, benzene, cadmium, chlorpyrifos-methyl, bisphenol A, phthalates, phenols, and bile acids. Recent technological advancements, including next-generation sequencing technologies and novel computational algorithms, have enabled the profiling and functional characterizations of lncRNAs on a genomic scale. In this review, we summarize the biogenesis and general biological functions of lncRNAs, highlight the important roles of lncRNAs in human diseases and especially during the toxicological responses to various xenobiotics, evaluate current methods for identifying aberrant lncRNA expression and molecular target interactions, and discuss the potential to implement these tools to address fundamental questions in toxicology.

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Section: Lncrnas and Heavy Metalsmentioning

confidence: 99%

Long Non-Coding RNAs: A Novel Paradigm for Toxicology

Dempsey

Cui

2016

Toxicol. Sci.

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“…The full-length cDNA of CRNG was obtained via RACE. Simultaneously, for identifying the characteristic of CRNG, genome location and coding potential were analyzed via NCBI Genome and ORF Finder (Zhang et al, 2012), CPC (Kong et al, 2007), respectively. Additionally, the ORF finder can search novel DNA sequence for potential protein encoding fragments, then verify predicted protein via SMART BLAST or regular BLASTP.…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine

Huang

et al. 2019

Front. Pharmacol.

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Our previous study has showed that a novel gene is differentially expressed in the liver of cyadox-fed piglets, but its sequence and function are unknown. Here, rapid amplification of cDNA ends (RACE) and bioinformatics analysis showed that the novel gene is 953 bp without protein-coding ability and locates in chromosome 11. Hence, we identified the novel gene as long non-coding RNA (lncRNA) and named it cyadox-related novel gene (CRNG). Fluorescence in situ hybridization (FISH) showed that CRNG mainly distributes in cytoplasm. Moreover, microarray assay in combination with CRNG interference and overexpression showed that the differential genes such as ANPEP, KITLG, STAT5A, FOXP3, miR-451, IL-2, IL-10, IL-6, and TNF-α are mainly involved in viral and pathogens infection and the immune-inflammatory responses in PK-15 cells. This work reveals that CRNG might play a role in preventing the host from being infected by pathogens and viruses and exerting immune regulatory effects in the cytoplasm, which may be involved in prophylaxis of cyadox in piglets.

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“…Many lncRNAs have been reported to be associated with non-small cell lung cancer (NSCLC), such as CAR10, MALAT1, HOTAIR and so on [32][33][34]. Studies have shown that lncRNA was also involved in nickel-induced tumorigenesis [35,36]. A new lncRNA, termed nickel-related gene (NRG1), was the first to be identified as a lncRNA induced in Ni-induced lung cancer but the mechanisms that regulate nickel-induced NRG1 level is not known [37].…”

Section: Non-coding Rnamentioning

confidence: 99%

The Role of Non-Coding RNAs Involved in Nickel-Induced Lung Carcinogenic Mechanisms

Zhu

Chen

et al. 2019

Inorganics

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Nickel is a naturally occurring element found in the Earth’s crust and an International Agency for Research on Cancer (IARC)-classified human carcinogen. While low levels found in the natural environment pose a minor concern, the extensive use of nickel in industrial settings such as in the production of stainless steel and various alloys complicate human exposure and health effects. Notably, interactions with nickel macromolecules, primarily through inhalation, have been demonstrated to promote lung cancer. Mechanisms of nickel-carcinogenesis range from oxidative stress, DNA damage, and hypoxia-inducible pathways to epigenetic mechanisms. Recently, non-coding RNAs have drawn increased attention in cancer mechanistic studies. Specifically, nickel has been found to disrupt expression and functions of micro-RNAs and long-non-coding RNAs, resulting in subsequent changes in target gene expression levels, some of which include key cancer genes such as p53, MDM2, c-myc, and AP-1. Non-coding RNAs are also involved in well-studied mechanisms of nickel-induced lung carcinogenesis, such as the hypoxia-inducible factor (HIF) pathway, oxidative stress, DNA damage and repair, DNA hypermethylation, and alterations in tumor suppressors and oncogenes. This review provides a summary of the currently known epigenetic mechanisms involved in nickel-induced lung carcinogenesis, with a particular focus on non-coding RNAs.

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Isolation and Characterization of a Novel Noncoding RNA from Nickel-Induced Lung Cancer

Cited by 8 publications

References 28 publications

Long Non-Coding RNAs: A Novel Paradigm for Toxicology

Long Non-Coding RNAs: A Novel Paradigm for Toxicology

Molecular Characterization and Biological Function of a Novel LncRNA CRNG in Swine

The Role of Non-Coding RNAs Involved in Nickel-Induced Lung Carcinogenic Mechanisms

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