Isolation and characterization of a bovine isolate of Neospora caninum with low virulence

Rojo-Montejo, Silvia; Collantes-Fernández, Esther; Regidor‐Cerrillo, Javier; Álvarez‐García, Gema; Marugán-Hernández, Virginia; Pedraza-Dı́az, Susana; Blanco-Murcia, Javier; Prenafeta, Antoni; Ortega‐Mora, Luis Miguel

doi:10.1016/j.vetpar.2008.10.009

Cited by 67 publications

(89 citation statements)

References 34 publications

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“…Observação semelhante ocorreu em estudo realizado com gado leiteiro, em Madrid (14) . Apesar da literatura consultada (10) citar comumente a ocorrência de sinais clínicos em bovinos infectados, a existência de foco de infecção, sem sintomatologia característica, demonstra a possibilidade de a prevalência dentro do rebanho aumentar sem ser notada (5) .…”

Section: Resultsunclassified

SOROEPIDEMIOLOGIA DE Neospora caninum E Toxoplasma gondii EM BOVINOS DA RAÇA CURRALEIRO

et al. 2017

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ResumoEste estudo comparou a soroprevalência de N. caninum e T. gondii em bovinos Curraleiros ao de outras raças bovinas (Nelore, Girolando, Guzerá e Caracu). Foram utilizadas 119 fêmeas bovinas adultas provenientes de quatro propriedades localizadas nos estados de Goiás e Tocantins e na divisa entre Goiás, Minas Gerais e Bahia. Animais soropositivos para N. caninum foram encontrados em todas as propriedades e o número de Curraleiros reagentes foi significativamente maior em relação aos bovinos Guzerá e Nelore. Também foram identificados anticorpos anti-T. gondii nos animais em todas as propriedades. A prevalência de soropositivos para T. gondii foi semelhante entre Curraleiros e bovinos das raças Nelore, Girolando e Guzerá; entretanto, foi significativamente maior em relação à raça Caracu. Palavras-chave: bovino; neosporose; Pé-duro; raças locais; toxoplasmose. AbstractThis study compared the health status of Curraleiro cattle and other breeds (Nellore, Girolando, Guzera, and Caracu) for toxoplasmosis and neosporosis screening against anti-Neospora caninum and anti-Toxoplasma gondii antibodies, respectively. We used 119 female bovines of Curraleiro, Guzera, Nellore, Caracu, and Girolando breeds from four farms in Goias and Tocantins states and in the border of Goias, Minas Gerais, and Bahia states. All the farms had seropositive bovines for N. caninum. The number of seropositive Curraleiro cattle was significantly higher than the number of Guzera and Nellore cattle. Also, antibodies against toxoplasmosis were found in animals from all properties. There was no significant difference between the number of seropositive Curraleiro cattle

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Section: Resultsunclassified

SOROEPIDEMIOLOGIA DE Neospora caninum E Toxoplasma gondii EM BOVINOS DA RAÇA CURRALEIRO

et al. 2017

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“…Em vacas prenhes, observa-se morte fetal quando a infecção ocorre no início da gestação (Barr et al, 1994;Williams et al, 2007;Rojo-Montejo et al, 2009), abortamentos ou nascimento de animais infectados quando a infecção ocorre no segundo terço (Dubey et al, 1992b;Gondim et al, 2004a;Almería et al, 2010), e no últi-mo terço da gestação, comumente nascem animais assintomáticos, porém congenitamente infectados (Williams et al, 2000;Gibney et al, 2008).…”

Section: Sinais Clínicosunclassified

Os parasitas de ovinos

Amarante¹,

Ragozo²,

Silva³

2014

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“…Recently, the isolation of a considerable number of N. caninum isolates from clinically healthy calves in Spain was reported , Rojo-Montejo et al 2009). Here, we describe the isolation and biological characterisation of a new N. caninum isolate, Nc-Goiás 1, obtained from an asymptomatically, infected calf in Brazil.…”

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“…Debora Pereira asymptomatic calves, such as JPA1, Nc-Nowra or Nc-Spain 1H (Shibahara et al 1999, Miller et al 2002, Rojo-Montejo et al 2009). Further studies with a higher number of isolates obtained from infected but clinically healthy animals using naturalhost infection models are needed to determine the real influence of virulence inherent to the isolate on the outcome of N. caninum infection in cattle and the differences between isolates from clinically affected and healthy animals.…”

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Isolation and biological characterisation of a new isolate of Neospora caninum from an asymptomatic calf in Brazil

García-Melo¹,

Regidor‐Cerrillo

Ortega‐Mora

et al. 2009

Acta Parasitologica

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Neospora caninum is a tissue-cyst forming parasite that has been recognized worldwide as a cause of abortion in cattle. Despite the ubiquitous distribution of this parasite and its broad range of hosts, the number of N. caninum isolates obtained to date is limited. In addition, the majority of isolates have been obtained from clinically affected hosts, therefore potentially biasing this population towards more virulent isolates. This report describes the isolation and biological characterisation of a new N. caninum isolate, Nc-Goiás 1, obtained from an asymptomatic, naturally infected calf from Brazil. This new isolate was identified as a member of the N. caninum species by polymerase chain reaction (PCR) using specific primers based on the N. caninum internal transcribed spacer 1 (ITS-1) sequence, and was genetically identified at multiple loci using microsatellite analysis. Finally, a pathogenicity study was conducted in a BALB/c mice model. All Nc-Goiás 1-infected mice survived without exhibiting any clinical signs. Further pathogenic characterisation of this isolate suggested that Nc-Goiás 1 is less virulent than other N. caninum isolates (Nc-Liv and Nc-1) studied in this mouse model. This is the first report of the isolation and biological characterisation of N. caninum from an infected but clinically healthy calf in South America.

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Isolation and characterization of a bovine isolate of Neospora caninum with low virulence

Cited by 67 publications

References 34 publications

SOROEPIDEMIOLOGIA DE Neospora caninum E Toxoplasma gondii EM BOVINOS DA RAÇA CURRALEIRO

SOROEPIDEMIOLOGIA DE Neospora caninum E Toxoplasma gondii EM BOVINOS DA RAÇA CURRALEIRO

Os parasitas de ovinos

Isolation and biological characterisation of a new isolate of Neospora caninum from an asymptomatic calf in Brazil

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