Isolation and characterization of a cDNA from Quercus robur differentially expressed in juvenile-like and mature shoots

Gil, Beatriz Gutiérrez; Pastoriza, Eva; Ballester, A.; Sánchez, Conchi

doi:10.1093/treephys/23.9.633

Cited by 23 publications

(15 citation statements)

References 34 publications

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“…Assessment of rooting in cuttings of pedunculate oak allowed the detection of 10 QTLs explaining between 4 and 13.8% of the phenotypic variance, of which the strongest were stable across years (Scotti- . Furthermore, a full-length cDNA clone named QrCPE, which is differentially expressed during in vitro culture of mature (difficult-to-root) and juvenile (easy-to-root) shoots of pedunculate oak was identified by differential display (Gil et al 2003). The deduced amino acid sequence showed that QrCPE encodes a small putative cell wall protein that is rich in glycine and histidine residues.…”

Section: Genomicsmentioning

confidence: 99%

Application of biotechnological tools to Quercus improvement

et al. 2011

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The genus Quercus, which belongs to the family Fagaceae, is native to the northern hemisphere and includes deciduous and evergreen species. The trees of the different species are very important from both economic and ecological perspectives. Application of new technological approaches (which span the fields of plant developmental biology, genetic transformation, conservation of elite germplasm and discovery of genes associated with complex multigenic traits) to these long-rotation hardwoods may be of interest for accelerating tree improvement programs. This review provides a summary of the advances made in the application of biotechnological tools to specific oak species. Significant progress has been made in the area of clonal propagation via organogenesis and somatic embryogenesis (SE). Standardized procedures have been developed for micropropagating the most important European (Q. robur, Q. petarea, Q. suber) and American (Q. alba, Q. bicolor, Q. rubra) oaks by axillary shoot growth. Although regenerated plantlets are grown in experimental trials, large-scale propagation of oak species has not been carried out. The induction of SE in oaks from juvenile explants is generally not problematic, although the use of explants other than zygotic embryos is much less efficient. During the last decade, enormous advances have been made in inducing SE from selected adult trees, mainly specimens of pedunculate oak (Q. robur) and cork oak (Q. suber). Advances in the understanding of the maturation and germination steps are required for better use of embryogenic process in clonal forestry. Quercus species are late-maturing and late-flowering, exhibit irregular seed set, and produce seeds that are recalcitrant to storage by conventional procedures. Vitrification-based cryopreservation techniques were used successfully in somatic embryos of pedunculate oak and cork oak, and an applied genbank of cork oak selected genotypes is now under development. The feasibility of genetic transformation of pedunculate oak and cork oak somatic embryos by means of co-culture techniques with several strains of Agrobacterium tumefaciens has also been demonstrated. To date, most research on the genomics of Quercus species has concerned population genetics. Approaches using functional genomics to examine the molecular and cellular mechanisms that control organogenesis and or somatic embryogenesis are still scarce, and efforts on the isolation and characterization of genes related to other specific traits should be intensified in the near future, as this would help improve the practical application of clonal forestry in recalcitrant species such as oaks.

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Section: Genomicsmentioning

confidence: 99%

Application of biotechnological tools to Quercus improvement

et al. 2011

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“…The present findings revealed that the rooting stage could serve as a suitable marker of phase changes. Genes are expressed differently at the multiplication stage and at the end of rooting stage as reported in oak species using cDNA characterization (Gil et al 2003).…”

Section: Discussionmentioning

confidence: 92%

Micromorphological response towards altered environmental conditions in subsequent stages of in vitro propagation of Morinda coreia

Shekhawat¹,

Manokari²,

Kannan³

2017

EEB

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The foliar micromorphological alterations from in vitro to field environments of micropropagated plantlets of Morinda coreia Buch. and Ham. were compared as developmental changes across the culture conditions. There were gradual developmental responses in stomatal structures, vascular tissues (venation) and raphides towards the field environment. The leaves under in vitro environment were thin with poorly developed leaf characteristics; these were impaired through ex vitro rooting prior to field transplantation. In vitro leaves had lower vein islet density (8.6 ± 0.16) and vein terminations (0.0) and raphide density (8.0 ± 0.19) as compared to rooted and acclimatized leaves. The physiological and structural adaptations in terms of stomata development lead to effective regulation of transpiration. Improved vein density for proper translocation of nutrients was achieved during gradual acclimation. Development of crystals, raphides and trichomes in the field environment suggested improvement in defense mechanisms. The gradual changes in foliar micromorphological structures during subsequent stages were responsible for adaptability, leading to improved survival success of in vitro regenerated plantlets of M. coreia during their field trials.

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“…Esto estaría dado por una pérdida de competencia morfogénica en el material adulto asociada a diferencias morfológicas, bioquímicas y moleculares (Gil et al 2003).…”

Section: Resultados Y Discusiónunclassified