Isolation and characterization of a thermostable cellulase-producing Fusarium chlamydosporum

Qin, Yongling; He, Hui; Li, Nan; Ling, Min; Liang, Zhiqun

doi:10.1007/s11274-010-0383-x

Cited by 32 publications

(18 citation statements)

References 28 publications

(31 reference statements)

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“…For examples, Kwon et al used esculin to visualize β-glucosidase activity on Native-PAGE. The gel was removed and soaked in solution containing 0.1% esculin and 0.03% ferric ammonium citrate until a dark band appeared on the gel indicating the presence of β-glucosidase activity [139,166,167]. P-PNG has also been utilized to detect β-glucosidase activity on PAGE.…”

Section: Zymographymentioning

confidence: 99%

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Ahmed¹,

Aslam²,

Ashraf³

et al. 2017

JAEM

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Cellulose is the most abundant biomaterial in the biosphere and the major component of plant biomass.Cellulase is an enzymatic system required for conversion of renewable cellulose biomass into free sugar for subsequent use in different applications. Cellulase system mainly consists of three individual enzymes namely: endoglucanase, exoglucanase and β-glucosidases. β-Glucosidases are ubiquitous enzymes found in all living organisms with great biological significance. β-Glucosidases have also tremendous biotechnological applications such as biofuel production, beverage industry, food industry, cassava detoxification and oligosaccharides synthesis. Microbial β-glucosidases are preferred for industrial uses because of robust activity and novel properties exhibited by them. This review aims at describing the various biochemical methods used for screening and evaluating β-glucosidases activity from microbial sources. Subsequently, it generally highlights techniques used for purification of β-glucosidases. It then elaborates various biochemical and molecular properties of this valuable enzyme such as pH and temperature optima, glucose tolerance, substrate specificity, molecular weight, and multiplicity. Furthermore, it describes molecular cloning and expression of bacterial, fungal and metagenomic β-glucosidases. Finally, it highlights the potential biotechnological applications of β-glucosidases.

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Section: Zymographymentioning

confidence: 99%

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Ahmed¹,

Aslam²,

Ashraf³

et al. 2017

JAEM

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“…And the results that maximum cellulase activities occurred at different points in different substrates, were probably due to the different expression of enzymes, which might influence the synergistic action towards the insoluble cellulose. Thus, these strains produced all three required components of the cellulase enzyme complex, which hydrolyzed crystalline cellulose into oligosaccharides first and ultimately into glucose (Qin et al 2010). Currently, most commercial cellulases are produced by Trichoderma species and Aspergillus species ).…”

Section: Optimum Fermentation Timementioning

confidence: 99%

Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains

Ling¹,

Kan²,

Yan³

et al. 2012

Electron. J. Biotechnol.

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The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28ºC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40ºC and pH 4.0, respectively, and more than 50% of its maximal activity was retained at 40-60ºC for 1 hr, while approximately 40% of its maximal activity was also retained after incubating at 70ºC for 1 hr. Most metal ions and reagents such as Ca 2+ , Mg 2+ , Cd 2+ , Pb 2+ , Zn 2+ , Cu 2+, EDTA, and SDS inhibited the enzyme activities, but K + and Mn 2+ activated the activities. The enzymes from Bacillus thuringiensis strains could be applied in bioconversion of lignocellulosic biomass into fermentable sugars.

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“…3B, which was similar to cellobiohydrolases from other thermophilic fungi, such as Fusarium chlamydosporum, Thermoascus aurantiacus and Thielavia terrestris. (Qin et al, 2010;Hong et al, 2003;Woon et al, 2016). The bivalent metallic cations Mg 2+ and Ca 2+ significantly enhanced the activity of CtCel6, while the monovalent cations K + and Na + were not detected with obvious effect (Table 1).…”

Section: Discussionmentioning

confidence: 95%

A Novel Application Potential of GH6 Cellobiohydrolase CtCel6 from Thermophilic Chaetomium thermophilum for Gene Cloning, Heterologous Expression and Biological Characterization

Zhou¹,

Jia²,

Ji³

et al. 2017

IJAB

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Chaetomium thermophilum is a thermophilic fungus expressed a series of glycoside hydrolases. Genome sequence analysis of C. thermophilium revealed that ctcel6 gene encoded a putative cellobiohydrolase which composed of 397 amino acid residues including a predicted signal peptide sequence. Ctcel6 gene was cloned, heterologously expressed in Pichia pastoris and purified by Ni 2+ affinity chromatography. Sequence alignment indicated that CtCel6 enzyme belonged to glycoside hydrolase family 6 (GH6) and the molecular mass of purified recombinant enzyme CtCel6 was 42 kDa by SDS-PAGE analysis. Characterization of recombinant CtCel6 exhibited high hydrolysis activity and excellent thermostability. The optimum reaction temperature and pH was 70 o C and pH 5, respectively. The bivalent metallic cations Mg 2+ and Ca 2+ significantly enhanced the activity of CtCel6. The specific activity of CtCel6 enzyme was 1.27 U/mg and Km value was 0.38 mM on β-Dglucan. The substrate specificity and hydrolysis products insisted that CtCel6 was an exo-/endo-type cellobiohydrolase. The biochemical properties of recombinant CtCel6 made it potentially effective for bioconversion of biomass and had tremendous potential in industrial applications such as enzyme preparation industry and feed processing industry.

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Isolation and characterization of a thermostable cellulase-producing Fusarium chlamydosporum

Cited by 32 publications

References 28 publications

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains

A Novel Application Potential of GH6 Cellobiohydrolase CtCel6 from Thermophilic Chaetomium thermophilum for Gene Cloning, Heterologous Expression and Biological Characterization

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