Isolation and Characterization of a Cold-Tolerant Strain of <i>Fusarium proliferatum</i>, a Biocontrol Agent of Grape Downy Mildew

Bakshi, Shlomo; Sztejnberg, A.; Yarden, Oded

doi:10.1094/phyto.2001.91.11.1062

Cited by 36 publications

(25 citation statements)

References 29 publications

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“…They appear to be frequent components of almost every endophyte survey, regardless the type of plant analysed. Other taxa present in our collection such as Alternaria alternata or Fusarium proliferatum have also been identified as promising biocontrol agents against Distribution of isolated fungi between the several plant parts analyzed; a: mean numbers of isolated species per tissue sample; b: mean numbers of isolated strains per tissue sample specific grapevine pathologies like grapevine downy mildew caused by Plasmopara viticola (Musetti et al 2006;Falk et al 1996;Bakshi et al 2001), and were also detected in our study. Among all these fungal antagonists, A. pullulans, A. alternata, E. nigrum and F. proliferatum were the most abundant taxa recovered in our study.…”

Section: Discussionsupporting

confidence: 67%

The endophytic mycota associated with Vitis vinifera in central Spain

García¹,

Tello²

2010

Fungal Diversity

177

120

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This study investigates the diversity of fungal endophytes associated with several varieties of grapevines with differing modes of cultivation in the Madrid region (central Spain). Our goal is to screen for and identify new fungal biocontrol agents against vine diseases, especially those associated with young plants produced in nurseries. A total of 500 fungal strains representing 68 taxa from six locations were isolated and characterised. Differences regarding cultivar and plant part processed were analysed in terms of composition and relative abundance of species. Some of the more frequently isolated strains represented were Acremonium, Alternaria, Aureobasidium, Botryotinia, Cladosporium, Epicoccum, Fusarium, Gibberella, Nectria, Penicillium, Phoma and Trichoderma species. Botryosphaeria species and Phomopsis viticola were also frequently isolated and may be vine pathogens. Several species of Acremonium, Phoma (P. glomerata) and Chaetomium showed promising antagonistic activity at the laboratory scale.

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Section: Discussionsupporting

confidence: 67%

The endophytic mycota associated with Vitis vinifera in central Spain

García¹,

Tello²

2010

Fungal Diversity

177

120

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“…Due to their high costs and difficulties in application and effectiveness, only a few biocontrol agents are used successfully against diseases (5,6,19). In particular, several organisms have shown promising results as biocontrol agents against grapevine downy mildew, i.e., Epicoccum nigrum (29), Acremonium byssoides (9), and Fusarium proliferatum (3,21).…”

Section: Additional Keyword: Antagonistsmentioning

confidence: 99%

Inhibition of Sporulation and Ultrastructural Alterations of Grapevine Downy Mildew by the Endophytic Fungus Alternaria alternata

Musetti¹,

Vecchione²,

Stringher³

et al. 2006

Phytopathology®

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One hundred twenty-six endophytic microorganisms isolated from grapevine leaves showing anomalous symptoms of downy mildew were tested on grapevine leaf disks as biocontrol agents against Plasmopara viticola. Among the 126 microorganisms, only five fungal isolates completely inhibited the sporulation of P. viticola; all of them were identified as Alternaria alternata. Ultrastructural analyses were carried out by transmission electron microscopy to observe cellular interactions between P. viticola and A. alternata in the grapevine leaf tissue. Cytological observations indicated that, even without close contact with A. alternata, the P. viticola mycelium showed severe ultrastructural alterations, such as the presence of enlarged vacuoles or vacuoles containing electron-dense precipitates. Haustoria appeared necrotic and irregularly shaped or were enclosed in callose-like substances. Therefore, a toxic action of A. alternata against P. viticola was hypothesized. To examine the production of toxic low-molecular-weight metabolites by A. alternata, we analyzed the fungal liquid culture by thin layer chromatography and proton magnetic resonance spectroscopy. The main low-molecular-weight metabolites produced by the endophyte were three diketopiperazines: cyclo(l-phenylalanine-trans-4-hydroxy-l-proline), cyclo(l-leucine-trans-4-hydroxy-l-proline), and cyclo(l-alanine-trans-4-hydroxy-l-proline). When applied at different concentrations to both grapevine leaf disks and greenhouse plants, a mixture of the three diketopiperazines was very efficacious in limiting P. viticola sporulation.

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“…& Curtis) Berl. & De Toni (Falk et al 1996;Kortekamp 1997;Bakshi et al 2001;Assante et al 2005;Musetti et al 2006;Burruano et al 2008). In this regard, Acremonium sp.…”

Section: Introductionmentioning

confidence: 99%

Identification of Acremonium isolates from grapevines and evaluation of their antagonism towards Plasmopara viticola

et al. 2015

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Some endophytic fungal genera in Vitis vinifera, including Acremonium, have been reported as antagonists of Plasmopara viticola. Endophytic Acremonium isolates from an asymptomatic grapevine cultivar Inzolia from Italy were identified by morphological features and multigene phylogenies of ITS, 18S and 28S genes, and their intra-specific genomic diversity was analyzed by RAPD analysis. Culture filtrates (CFs) obtained from Acremonium isolates were tested in vitro for their inhibitory activity against the P. viticola sporangia germination. Among 94 isolates, 68 belonged to the Acremonium persicinum and 26 to the Acremonium sclerotigenum. RAPD analysis grouped the A. persicinum isolates into 15 clusters and defined 31 different strains. The A. sclerotigenum isolates, instead, were clustered into 22 groups and represented 25 strains. All A. persicinum CFs inhibited sporangia germination of P. viticola, while not all those of A. sclerotigenum had inhibitory effect. A different degree of inhibition was observed between strains of the same species, while some strains of different species showed identical inhibitory effect. No correlation was found between RAPD groups and inhibitory activity in both Acremonium species.

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Isolation and Characterization of a Cold-Tolerant Strain of Fusarium proliferatum, a Biocontrol Agent of Grape Downy Mildew

Cited by 36 publications

References 29 publications

The endophytic mycota associated with Vitis vinifera in central Spain

The endophytic mycota associated with Vitis vinifera in central Spain

Inhibition of Sporulation and Ultrastructural Alterations of Grapevine Downy Mildew by the Endophytic Fungus Alternaria alternata

Identification of Acremonium isolates from grapevines and evaluation of their antagonism towards Plasmopara viticola

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