“…The E. coli strain used in this study, the minicell-producing strain P678-54 (1), has the following markers: thr, ara, leu, azi, tonA, lacY, minA, gal, minB, rpsL, malA, xyl, mtl, thi, supE. The CloDF13 cop-3 replication control mutant has been described previously (13,37). All transposon mutants used in this study and listed in Table 1 (35).…”