2014
DOI: 10.3136/fstr.20.1101
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Isolation and Characterization of a Novel Mannose- and Fructose-Binding Lectin from the Edible Wild Mushroom Hygrophorus russula (Fr.) Quél.

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Cited by 3 publications
(6 citation statements)
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“…Owing to differential binding strength on the Q-Sepharose and SP-Sepharose exchange columns, the lectin ABL could be purified from the crude protein solutions with an acceptable recovery (harvest) rate of 9.0% which is quite higher than that of other lectins from mushroom Stropharia rugosoannulata (7.9%) [11], Hygrophorus russula (1.2%) [13], etc. Many reported lectins can be adsorbed on both anion exchange chromatography as DEAE-Cellulose and Q-Sepharose and cation exchange chromatography as CM-Cellulose and SP-Sepharose.…”
Section: Discussionmentioning
confidence: 99%
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“…Owing to differential binding strength on the Q-Sepharose and SP-Sepharose exchange columns, the lectin ABL could be purified from the crude protein solutions with an acceptable recovery (harvest) rate of 9.0% which is quite higher than that of other lectins from mushroom Stropharia rugosoannulata (7.9%) [11], Hygrophorus russula (1.2%) [13], etc. Many reported lectins can be adsorbed on both anion exchange chromatography as DEAE-Cellulose and Q-Sepharose and cation exchange chromatography as CM-Cellulose and SP-Sepharose.…”
Section: Discussionmentioning
confidence: 99%
“…A lectin from a wild toxic mushroom Inocybe umbrinella , which is from the same order as A. bitorquis , was purified with a recovery rate of 11% following a protocol comprised ion exchange chromatography on DEAE-Cellulose, and CM-Cellulose, and gel filtration on Superdex 75 [19]. However, H. russula lectin was isolated with a very low recovery rate of 1.2% and a long purification procedure encompassing five successive ion exchange chromatography on CM-Cellulose, DEAE-Cellulose, Q-Sepharose, SP-Sepharose and Mono S [13]. Therefore, simple and efficient purification processes are the important guarantees for protein purification and the recovery rate.…”
Section: Discussionmentioning
confidence: 99%
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“…The hemagglutinating activity tests were performed according to the method of Liu et al [32], with 96-well microtiter plates (Molecular Devices, Sunnyvale, CA, USA). A hemagglutinating unit (U) is defined as the lowest concentration of lectin that exhibiting hemagglutinating activity.…”
Section: Assay Of Hemagglutinating Activitymentioning
confidence: 99%