Isolation and characterization of a polygalacturonase gene highly expressed in Brassica napus pollen

Robert, Laurian S.; Allard, Sharon; Gerster, Jean L.; Cass, Leslie; Simmonds, John

doi:10.1007/bf00042360

Cited by 57 publications

(46 citation statements)

References 21 publications

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“…2) which is less than the 42.4 kDa weight estimated for this protein (Robert et al 1993). This major band was present in unpollinated stigmas but at a much lower level.…”

Section: Immunoblotting Of Pg Enzyme In Tissuesmentioning

confidence: 61%

“…This sequence is a combination of an internal peptide and the C-terminus of the B. napus cv Westar PG published by Robert et al (1993). The peptide was custom synthesised by Mimotopes (Clayton, Melbourne, Victoria, Australia) while the antiserum was raised in rabbits by the Institute of Medical and Veterinary Science (Veterinary Services Division, Adelaide, Australia).…”

Section: Production Of Pg Antibodymentioning

confidence: 99%

“…We have used a polyclonal antibody generated to a published sequence of a PG enzyme highly expressed in Brassica napus pollen (Robert et al 1993) to determine the timing of expression of PG enzyme and the spatial localisation of the enzyme in germinating pollen in vivo. We have discovered that although the levels of PG enzyme did not detectably rise in germinated pollen, the enzyme was found in the tip of the pollen tube during papillar cell penetration.…”

Section: Introductionmentioning

confidence: 99%

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Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus

Dearnaley¹,

Daggard²

2001

Sexual Plant Reproduction

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Penetration of pollen tubes through stigmatic tissues in Brassica napus L. may involve the release of cell wall modifying enzymes from the pollen tube tip. We have examined the expression of a pectin-degrading polygalacturonase (PG) enzyme in unpollinated and early and late pollinated stigmas via immunoblotting and immuno light microscopy using a PG polyclonal antibody. Immunoblotting analysis indicated that PG enzyme was present at low levels in unpollinated stigmas and at high levels in pollinated stigmas. The level of PG did not detectably increase between early and late pollinated stigmas. Immuno light microscopy demonstrated that PG enzyme was present in ungerminated pollen grains, stigmatic papillae and in the tip of pollen tubes growing into the papillar wall. This latter evidence suggests that PG enzyme may play an important role in papillar cell wall penetration during pollination although other interpretations of the role of pollen PG should not be discounted.

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“…2) which is less than the 42.4 kDa weight estimated for this protein (Robert et al 1993). This major band was present in unpollinated stigmas but at a much lower level.…”

Section: Immunoblotting Of Pg Enzyme In Tissuesmentioning

confidence: 61%

Section: Production Of Pg Antibodymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus

Dearnaley¹,

Daggard²

2001

Sexual Plant Reproduction

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“…To obtain full-length clones, Sta 41 was used as a probe to screen 20 000 plaques of a stamen cDNA library (Robert et al, 1993). Approximately 0.1% of the plaques hybddized with the Sta 41 probe at high stringency (results not shown).…”

Section: Isolation Of Cdnasmentioning

confidence: 99%

“…The plant material was descdbed previously (Robert et al, 1993). Total RNA was extracted according to Ouellet et al (1992).…”

Section: Northern Blot Analysismentioning

confidence: 99%

Molecular characterization of two Brassica napus genes related to oleosins which are highly expressed in the tapetum

Robert¹,

Gerster²,

Allard

et al. 1994

The Plant Journal

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SummaryTwo highly homologous Brassica napus flower cDNA clones, Sta 41-2 and Sta 41-9, were isolated and characterized. These clones were shown to correspond to genes expressed in the tapetum from the early uninucleate microspore stage to the dinucleate stage. The predicted Sta 41-2 and Sta 41-9 proteins possessed characteristics similar to oleosins such as a polar N-terminal domain, a large relatively conserved hydrophobic domain and a long C-terminal domain which consisted of four different groups of repeats. In addition, like oleosins, the Sta 41-2 and Sta 41-9 proteins have a basic pl, lack a signal peptide and are found in a tissue which accumulates Iipids in small lipid bodies.

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The Developmental Biology of Pollen

Twell

2018

Annual Plant Reviews Online

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Contents X.1 Introduction Part 1: Cell biology and differentiation X.2 Microsporogenesis X.3 Microgametogenesis X.4 Sporophytic-gametophytic interactions X.4.1 The unique pollen wall X.4.2 Exine pattern mutants X.4.3 Phenylpropanoids X.4.4 The pollen coat X.4.5 Microspore nutrition and metabolism X.5 Microspore development X.5.1 Microspore isolation X.5.2 Vacuole morphogenesis, growth and polarity X.6 Asymmetric division, cell fate and polarity X.6.1 Asymmetric cytokinesis-sealing cell fate X.6.2 Microspore and pollen embryogenesis X.6.3 Models of cell-fate determination X.6.4 Determination of microspore polarity X.6.5 Mutants affecting microspore polarity, division and cell-fate X.7 Generative and sperm cell development X.7.1 Generative cell migration X.7.2 Generative cell morphogenesis X.7.3 Generative cell division X.7.4 The male germ unit X.7.4.1 Male germ unit morphogenesis Part 2: Molecular biology and genetics X.8 Male gametophytic gene expression X.8.1 Developmental phases of expression X.8.2 Transcription in developing microspores X.8.3 Transcription in developing pollen X.8.4 Pollen-specific regulatory elements X.8.5 Post-transcriptional regulation X.8.6 Gene expression in generative and sperm cells 3X.9 Male gametophytic gene functions X.9.1 Pectin degrading enzymes X.9.2 Cytoskeleton-related proteins X.9.2.1 Actin binding proteins X.9.2.2 GTPases X.9.2.3 Microtubules X.9.3 Transcriptional regulators X.9.4 Protein kinases X.9.4.1 Receptor-like kinases X.9.4.2 MAP kinases X.9.4.3 Ca 2+ -regulated protein kinases X.9.5 Pollen allergens X.9.6 Pollen wall proteins X.10 Advances in gametophytic genetics X.10.1 Pollen morphogenesis screens X.10.2 Segregation ratio distortion screens X.11 Conclusions and perspective

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Isolation and characterization of a polygalacturonase gene highly expressed in Brassica napus pollen

Cited by 57 publications

References 21 publications

Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus

Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus

Molecular characterization of two Brassica napus genes related to oleosins which are highly expressed in the tapetum

The Developmental Biology of Pollen

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