Isolation and characterization of a male sterility gene homolog BcMS2 from Chinese cabbagge-pak-choi that expressing in an anther-specific manner

Ming, Jun; Cao, Jiashu

doi:10.1007/s11033-007-9086-2

Cited by 9 publications

(5 citation statements)

References 31 publications

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“…napus using RT-PCR technique, and found that there existed an amino acid divergence between fertile S45B and sterile S45A, which may be the putative male sterility site in S45A. Male sterility gene homolog, designated BcMS2 , expressed only in stage III flower buds of male fertile Chinese cabbage-pak-choi “ZUBajh97-01B” and there were no detection in all organs of Polima cytoplasmic male sterility (CMS) line ‘Bpol97-05A’ and Ogura CMS line ‘Bogu97-06A’ [77]. In this study, MS2 members (Bra034793, Bra038691) were only expressed in Fer, whose TPM was 1766.48 and 480.76, respectively, and then it was suggested that the two genes were involved in the development of Fer pollens.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profile analysis of young floral buds of fertile and sterile plants from the self-pollinated offspring of the hybrid between novel restorer line NR1 and Nsa CMS line in Brassica napus

Yan

Dong

et al. 2013

BMC Genomics

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BackgroundThe fertile and sterile plants were derived from the self-pollinated offspring of the F1 hybrid between the novel restorer line NR1 and the Nsa CMS line in Brassica napus. To elucidate gene expression and regulation caused by the A and C subgenomes of B. napus, as well as the alien chromosome and cytoplasm from Sinapis arvensis during the development of young floral buds, we performed a genome-wide high-throughput transcriptomic sequencing for young floral buds of sterile and fertile plants.ResultsIn this study, equal amounts of total RNAs taken from young floral buds of sterile and fertile plants were sequenced using the Illumina/Solexa platform. After filtered out low quality data, a total of 2,760,574 and 2,714,441 clean tags were remained in the two libraries, from which 242,163 (Ste) and 253,507 (Fer) distinct tags were obtained. All distinct sequencing tags were annotated using all possible CATG+17-nt sequences of the genome and transcriptome of Brassica rapa and those of Brassica oleracea as the reference sequences, respectively. In total, 3231 genes of B. rapa and 3371 genes of B. oleracea were detected with significant differential expression levels. GO and pathway-based analyses were performed to determine and further to understand the biological functions of those differentially expressed genes (DEGs). In addition, there were 1089 specially expressed unknown tags in Fer, which were neither mapped to B. oleracea nor to B. rapa, and these unique tags were presumed to arise basically from the added alien chromosome of S. arvensis. Fifteen genes were randomly selected and their expression levels were confirmed by quantitative RT-PCR, and fourteen of them showed consistent expression patterns with the digital gene expression (DGE) data.ConclusionsA number of genes were differentially expressed between the young floral buds of sterile and fertile plants. Some of these genes may be candidates for future research on CMS in Nsa line, fertility restoration and improved agronomic traits in NR1 line. Further study of the unknown tags which were specifically expressed in Fer will help to explore desirable agronomic traits from wild species.

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Section: Discussionmentioning

confidence: 99%

Transcriptome profile analysis of young floral buds of fertile and sterile plants from the self-pollinated offspring of the hybrid between novel restorer line NR1 and Nsa CMS line in Brassica napus

Yan

Dong

et al. 2013

BMC Genomics

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“…Silencing of NTP303 family members (ntp101, 201, 302 and ntp805) in tobacco affected in vivo pollen tube growth and resulted in male sterile plants (de Groot et al 2004). Several ESTs, such as FB4, FB15, FB20, showed homology to male sterility-related protein from L. usitatissimum, Arabidopsis and Chinese cabbage (Aarts et al 1997;Jiang and Cao 2008;Chen et al 2011a, b). Male Sterile 2 encodes a plastid-localized fatty acyl carrier protein reductase required for pollen exine development in Arabidopsis (Chen et al 2011b).…”

Section: Discussionmentioning

confidence: 99%

“…FB3 and FB46 showed homology to a pollen development related gene, ntp805 (AY366400), in N. tabacum (Hafidh et al 2012). Several ESTs, such as FB4, FB15 and FB20 showed homology to male sterility-related protein from Linum usitatissimum, Arabidopsis and Chinese cabbage (Aarts et al 1997;Jiang and Cao 2008;Chen et al 2011b). F9 showed a similitude with pollen coat-like protein (ABM66381) from Camellia sinensis.…”

Section: Identification Of Genes Involved In Pollen Developmentmentioning

confidence: 99%

Transcriptional profiling analysis of genic male sterile–fertile Capsicum annuum reveal candidate genes for pollen development and maturation by RNA-Seq technology

Chen

Cao

et al. 2015

Plant Cell Tiss Organ Cult

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To identify genes involved in the pollen development and maturation, genome-wide transcriptional profiling of the flower buds from fertile and sterile plants of the genic male sterile-fertile line 114AB of Capsicum annuum was performed by the RNA-Seq approach. The results indicated that there were numerous changes in gene expression due to the gene mutation in sterile plants. A total of 668 differential expressed genes were identified. Among them, 562 were up-regulated and 106 were downregulated genes in the fertile plants compared to those of the sterile plants, including 202 and 2 genes specifically accumulated in fertile and sterile plants, respectively. Hundreds of genes potentially involved in pollen development were identified from the genes specifically expressed in the fertile plants, including pollen coat protein, lipid binding protein, lipid transfer protein, pectin methylesterase, male sterility-related protein, antherspecific protein, pectate lyase gene and so forth. Subsequent analysis of expression patterns for a subset of these genes were verified by semi-quantitative RT-PCR and quantitative RT-PCR. Furthermore, the up-regulated genes were predicted to be associated with intracellular membrane-bounded organelle, cytoplasmic vesicle, oxidoreductase activity, metal ion binding, pollen wall assembly, pollen exine formation, starch and sucrose metabolism, and other processes through Gene ontology and KEGG enrichment analysis. Taken together, this study provides a list of candidate genes and their expression patterns for pollen development and maturation in pepper.

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“…The study of anther development is important for Chinese cabbage breeding. Though several genes related to anther development have been identified in Chinese cabbage, such as BAN103 3 , BcMS2 4 , BcMF2 5 , BcMF5 6 , BcMF6 7 , BrSKS13 8 , BcMF12 9 , BcMF13 10 , BcMF19 11 , and BcMF20 12 , the molecular mechanism of anther development remains unstudied.…”

Section: Introductionmentioning

confidence: 99%

Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis)

Shi

Pang

Liu

et al. 2022

Sci Rep

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Anther development is precisely regulated by a complex gene network, which is of great significance to plant breeding. However, the molecular mechanism of anther development in Chinese cabbage is unclear. Here, we identified microRNAs (miRNAs), mRNAs, long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) related to anther development in Chinese cabbage (Brassica campestris L. ssp. pekinensis) to construct competitive endogenous RNA (ceRNA) regulatory networks and provide valuable knowledge on anther development. Using whole-transcriptome sequencing, 9055, 585, 1344, and 165 differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs), lncRNAs (DElncRNAs), and circRNAs (DEcircRNAs) were identified, respectively, in the anthers of Chinese cabbage compared with those in samples of the vegetative mass of four true leaves. An anther-related ceRNA regulatory network was constructed using miRNA targeting relationships, and 450 pairs of ceRNA relationships, including 97 DEmiRNA–DEmRNA, 281 DEmiRNA–DElncRNA, and 23 DEmiRNA–DEcircRNA interactions, were obtained. We identified important genes and their interactions with lncRNAs, circRNAs, and miRNAs involved in microsporogenesis, tapetum and callose layer development, pollen wall formation, and anther dehiscence. We analyzed the promoter activity of six predominant anther expression genes, which were expressed specifically in the anthers of Arabidopsis thaliana, indicating that they may play an important role in anther development of Chinese cabbage. This study lays the foundation for further research on the molecular mechanisms of anther growth and development in Chinese cabbage.

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Isolation and characterization of a male sterility gene homolog BcMS2 from Chinese cabbagge-pak-choi that expressing in an anther-specific manner

Cited by 9 publications

References 31 publications

Transcriptome profile analysis of young floral buds of fertile and sterile plants from the self-pollinated offspring of the hybrid between novel restorer line NR1 and Nsa CMS line in Brassica napus

Transcriptome profile analysis of young floral buds of fertile and sterile plants from the self-pollinated offspring of the hybrid between novel restorer line NR1 and Nsa CMS line in Brassica napus

Transcriptional profiling analysis of genic male sterile–fertile Capsicum annuum reveal candidate genes for pollen development and maturation by RNA-Seq technology

Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis)

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