Isolation and characterization of a thermophilic benzoate-degrading, sulfate-reducing bacterium, Desulfotomaculum thermobenzoicum sp. nov.

Tasaki, Masaharu; Kamagata, Yoichi; Nakamura, Kazunori; Mikami, Eiichi

doi:10.1007/bf00243454

Cited by 79 publications

(47 citation statements)

References 21 publications

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“…However, in the presence of sulfate as an electron acceptor, some sulfate-reducing bacterial species oxidize butyrate either completely to CO 2 or incompletely to acetate (Rabus et al, 2000). These sulfate-reducing bacterial species belong to the families Desulfobacteraceae (Cravo-Laureau et al, 2004;Kuever et al, 2005;Balk et al, 2008;Suzuki et al, 2008), Desulfohalobiaceae (Belyakova et al, 2006) and Syntrophobacteraceae (Beeder et al, 1995;Sievert & Kuever, 2000;Tanaka et al, 2000) in the class Deltaproteobacteria or to the genus Desulfotomaculum in the phylum Firmicutes (Daumas et al, 1988;Tasaki et al, 1991;Fardeau et al, 1995;Kuever et al, 1999;Vandieken et al, 2006).…”

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confidence: 99%

Desulfovibrio butyratiphilus sp. nov., a Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an anaerobic municipal sewage sludge digester

Suzuki

Ueki

Shizuku

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Strictly anaerobic, mesophilic, sulfate-reducing bacterial strains were isolated from two anaerobic municipal sewage sludge digesters. One representative strain (BSY T ) was characterized phenotypically and phylogenetically. Cells were Gram-negative, motile by means of a single polar flagellum, non-spore-forming, curved rods. Cells had desulfoviridin and cytochrome type c. Catalase and oxidase activities were not detected. The optimum NaCl concentration for growth was 0.5 % (w/v). The optimum temperature was 35 6C and the optimum pH was 7.1. Strain BSY T utilized butyrate, 2-methylbutyrate, valerate, pyruvate, lactate, ethanol, 1-propanol, butanol and H 2 as electron donors for sulfate reduction. This strain grew lithoautotrophically with H 2 /CO 2 under sulfate-reducing conditions. Most organic electron donors were incompletely oxidized to mainly acetate, whereas 2-methylbutyrate and valerate were oxidized to equivalent amounts of acetate and propionate. Strain BSY T utilized thiosulfate as an electron acceptor, and grew with pyruvate in the absence of electron acceptors. The genomic DNA G+C content of strain BSY T was 63.3 mol%. Menaquinone MK-6(H 2 ) was the major respiratory quinone. Major cellular fatty acids were C 14 : 0 , C 16 : 0 , C 16 : 1 v7 and C 18 : 1 v7. Phylogenetic analyses based on 16S rRNA and dissimilatory sulfite-reductase b-subunit gene sequences assigned strain BSY T to the genus Desulfovibrio in the family Desulfovibrionaceae within the class Deltaproteobacteria. Its closest recognized relative based on 16S rRNA gene sequences was the type strain of Desulfovibrio putealis (95.3 % similarity). On the basis of significant differences in 16S rRNA gene sequences and phenotypic characteristics, the sewage sludge strains are considered to represent a single novel species of the genus Desulfovibrio, for which the name Desulfovibrio butyratiphilus sp. nov. is proposed. The type strain is BSY T (5JCM 15519 T 5DSM 21556 T ).Butyrate is an important intermediate in the anaerobic degradation of organic matter in various anaerobic ecosystems. Because the oxidation of butyrate is usually thermodynamically unfavourable under anaerobic conditions, it is generally degraded by syntrophic interactions between H 2 -producing acetogenic bacteria and H 2 -utilizing methanogens in methanogenic conditions (Stams, 1994;Schink, 1997;Sekiguchi et al., 2000;Zhang et al., 2004). However, in the presence of sulfate as an electron acceptor, some sulfate-reducing bacterial species oxidize butyrate either completely to CO 2 or incompletely to acetate (Rabus et al., 2000). These sulfate-reducing bacterial species belong to the families Desulfobacteraceae (Cravo-Laureau et al., 2004;Kuever et al., 2005;Balk et al., 2008;Suzuki et al., 2008), Desulfohalobiaceae (Belyakova et al., 2006) and Syntrophobacteraceae (Beeder et al., 1995;Sievert & Kuever, 2000;Tanaka et al., 2000) in the class Deltaproteobacteria or to the genus Desulfotomaculum in the phylum Firmicutes (Daumas et al., 1988;Tasaki et al., 1991; Fardeau et al., 1...

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confidence: 99%

Desulfovibrio butyratiphilus sp. nov., a Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an anaerobic municipal sewage sludge digester

Suzuki

Ueki

Shizuku

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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“…thermobenzoicum and subsp. thermosyntrophicum (Tasaki et al, 1991;Plugge et al, 2002), D. thermocisternum (Nilsen et al, 1996), D. thermosapovorans (Fardeau et al, 1995) and D. thermosubterraneum (Kaksonen et al, 2006b). Additionally, the reclassification of 'Desulfotomaculum nigrificans subsp.…”

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confidence: 99%

Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater

Kaksonen

Spring

Schümann

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal-and sulfate-containing wastewater A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1 T , was rod-shaped and motile. The temperature range for growth was 33-51 6C (optimum 44-46 6C) and the pH range was 6.0-7.5 (optimum pH 6.4-7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C 15 : 0 , iso-C 17 : 1 v10c and iso-C 17 : 0 . Strain RE35E1 T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038 T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475 T (92.0 % similarity). Strain RE35E1 T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1 T (5DSM 16058 T 5JCM 14019 T ).The genus Desulfotomaculum constitutes a group of rodshaped, endospore-forming, sulfate-reducing bacteria. The genus currently includes mesophilic, moderately thermophilic and thermophilic species (Werkman & Weaver, 1927;Campbell & Postgate, 1965;Vandieken et al., 2006). et al., 1996), D. thermosapovorans (Fardeau et al., 1995) and D. thermosubterraneum (Kaksonen et al., 2006b). Additionally, the reclassification of 'Desulfotomaculum nigrificans subsp. salinus' as a novel species ('Desulfotomaculum salinum') has been proposed (Nazina et al., 2005). Currently, Desulfotomaculum arcticum is the only sulfate-reducing member of the genus that has an optimal temperature above 40 uC and below 50 uC (Vandieken et al., 2006). Recently, we reported the isolation of sulfate-reducers from fluidized-bed reactors (FBRs) treating acidic metal-and sulfate-containing wastewater (Kaksonen et al., 2004c). This paper describes one of those isolates, strain RE35E1 T , a novel, moderately thermophilic, spore-forming, sulfatereducing bacterium.Abbreviation: FBR, fluidized-bed reactor.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain RE35E1 T is AY548778.

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“…Characteristics of strain RL80JIV T in comparison with those of its closest phylogenetic relatives Taxa: 1, strain RL80JIV T ; 2, Thermanaeromonas toyohensis (Mori et al, 2002); 3, Desulfotomaculum thermocisternum (Nilsen et al, 1996); 4, Desulfotomaculum kuznetsovii (Nazina et al, 1989); 5, Desulfotomaculum luciae (Liu et al, 1997); 6, Desulfotomaculum thermobenzoicum subsp. thermobenzoicum (Tasaki et al, 1991); 7, Desulfotomaculum thermoacetoxidans (Min & Zinder, 1990); 8, Desulfotomaculum geothermicum (Daumas et al, 1988); 9, Desulfotomaculum solfataricum (Goorissen et al, 2003); 10, Desulfotomaculum australicum (Love et al, 1993). +, Positive; 2, negative; +/2, variable; NR, not reported.…”

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confidence: 99%

Desulfovirgula thermocuniculi gen. nov., sp. nov., a thermophilic sulfate-reducer isolated from a geothermal underground mine in Japan

Kaksonen

Spring

Schümann

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterial strain, designated RL80JIVT, was isolated from a geothermally active underground mine in Japan. Cells were rod-shaped and motile. The temperature and pH ranges for growth were 61–80 °C (optimum at 69–72 °C) and pH 6.4–7.9 (optimum at pH 6.8–7.3), and the strain tolerated up to 0.5 % NaCl. Strain RL80JIVT utilized sulfate, sulfite, thiosulfate and elemental sulfur as electron acceptors. Electron donors utilized were H2 in the presence of CO2, and carboxylic acids. Fermentative growth occurred on lactate and pyruvate. The cell wall contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone MK-7. Major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 0 and C16 : 0. Strain RL80JIVT was found to be affiliated with the thiosulfate-reducer Thermanaeromonas toyohensis DSM 14490T (90.9 % 16S rRNA gene sequence similarity) and with the sulfate-reducer Desulfotomaculum thermocisternum DSM 10259T (90.0 % similarity). Strain RL80JIVT is therefore considered to represent a novel species of a new genus, for which the name Desulfovirgula thermocuniculi gen. nov., sp. nov. is proposed. The type strain of Desulfovirgula thermocuniculi is RL80JIVT (=DSM 16036T=JCM 13928T).

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Isolation and characterization of a thermophilic benzoate-degrading, sulfate-reducing bacterium, Desulfotomaculum thermobenzoicum sp. nov.

Cited by 79 publications

References 21 publications

Desulfovibrio butyratiphilus sp. nov., a Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an anaerobic municipal sewage sludge digester

Desulfovibrio butyratiphilus sp. nov., a Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an anaerobic municipal sewage sludge digester

Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater

Desulfovirgula thermocuniculi gen. nov., sp. nov., a thermophilic sulfate-reducer isolated from a geothermal underground mine in Japan

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