Isolation and Characterization of a Drought-Induced Soybean cDNA Encoding a D95 Family Late-Embryogenesis-Abundant Protein

Maitra, Niranjan; Cushman, John C.

doi:10.1104/pp.106.2.805

Cited by 54 publications

(44 citation statements)

References 7 publications

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“…Plants of the sensitive genotype, BR16, showed increased expression of this gene in the last period of treatment, likely involving the same defense mechanism, albeit with different timing. The higher expression of Gmdefensin that we observed in the last period of treatment with the sensitive genotype, contrasts with the data of Maitra and Cushman (1998) that showed a 10-fold up-regulation in tolerant soybean plants under water deficiency. Yamada et al (2003) also reported an increase in the expression of a Gmdefensin homologue in Nicotiana excelsior under conditions of salinity stress, indicating involvement of this gene in responses to cell dehydration.…”

Section: Gm18srrna Gmdreb1acontrasting

confidence: 55%

Soybean physiology and gene expression during drought

Stolf-Moreira¹,

Medri²,

Neumaier³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. Soybean genotypes MG/BR46 (Conquista) and BR16, drought-tolerant and -sensitive, respectively, were compared in terms of morphophysiological and gene-expression responses to water stress during two stages of development. Gene-expression analysis showed differential responses in Gmdreb1a and Gmpip1b mRNA expression within 30 days of water-deficit initiation in MG/BR46 (Conquista) plants. Within 45 days of initiating stress, Gmp5cs and Gmpip1b had relatively higher expression. Initially, BR16 showed increased expression only for Gmdreb1a, and later (45 days) for Gmp5cs, Gmdefensin and Gmpip1b. Only BR16 presented down-regulated expression of genes, such as Gmp5cs and Gmpip1b, 30 days after the onset of moisture stress, and Gmgols after 45 days of stress. The faster perception of water stress in MG/BR46 (Conquista) and the better maintenance of up-regulated gene expression than in the sensitive BR16 genotype imply mechanisms by which the former is better adapted to tolerate moisture deficiency.

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Section: Gm18srrna Gmdreb1acontrasting

confidence: 55%

Soybean physiology and gene expression during drought

Stolf-Moreira¹,

Medri²,

Neumaier³

et al. 2010

Genet. Mol. Res.

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“…It is noteworthy that a group of plant defensins, apparently encoding proteinase inhibitors and known for the antifungal activity, also possess several conserved Cys clusters and an N-terminal signal sequence (Maitra and Cushman, 1998;Gao et al, 2000;van der Biezen, 2001). However, amino acid sequence comparisons between known plant defensins and CCPs revealed no similarities (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of Nodule-Specific Transcripts in the Model Legume Medicago truncatula

et al. 2002

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The Medicago truncatula expressed sequence tag (EST) database (Gene Index) contains over 140,000 sequences from 30 cDNA libraries. This resource offers the possibility of identifying previously uncharacterized genes and assessing the frequency and tissue specificity of their expression in silico. Because M. truncatula forms symbiotic root nodules, unlike Arabidopsis, this is a particularly important approach in investigating genes specific to nodule development and function in legumes. Our analyses have revealed 340 putative gene products, or tentative consensus sequences (TCs), expressed solely in root nodules. These TCs were represented by two to 379 ESTs. Of these TCs, 3% appear to encode novel proteins, 57% encode proteins with a weak similarity to the GenBank accessions, and 40% encode proteins with strong similarity to the known proteins. Nodule-specific TCs were grouped into nine categories based on the predicted function of their protein products. Besides previously characterized nodulins, other examples of highly abundant nodule-specific transcripts include plantacyanin, agglutinin, embryo-specific protein, and purine permease. Six nodule-specific TCs encode calmodulin-like proteins that possess a unique cleavable transit sequence potentially targeting the protein into the peribacteroid space. Surprisingly, 114 nodule-specific TCs encode small Cys cluster proteins with a cleavable transit peptide. To determine the validity of the in silico analysis, expression of 91 putative nodule-specific TCs was analyzed by macroarray and RNA-blot hybridizations. Nodule-enhanced expression was confirmed experimentally for the TCs composed of five or more ESTs, whereas the results for those TCs containing fewer ESTs were variable. , and organ development (Ruan et al., 1998;Girke et al., 2000; Zhu and Wang, 2000). Although Arabidopsis serves as the model system for most plant processes, it suffers from two major weaknesses in consideration of plant-microbe interactions: the absence of symbiotic associations with mycorrhizal fungi and with rhizobia.In recent years, Medicago truncatula and Lotus japonicus have emerged as model systems for genomic approaches to plant-microbe symbiotic associations (Barker et al., 1990;Handberg and Stougaard, 1992; Cook et al., 1997; Cook, 1999;Oldroyd and Geurts, 2001;Thoquet et al., 2002). Both species possess small genomes, are diploid, have fast generation times, and can be transformed with Agrobacterium tumefaciens and regenerated (Handberg and Stougaard, 1992; Blondon et al., 1994;Handberg et al., 1994; Chabaud et al., 1996;Jiang and Gresshoff, 1997;Stiller et al., 1997;Trinh et al., 1998;Trieu et al., 2000). Currently, both functional and structural genomics approaches are being pursued within each of these species. Covitz et al. (1998) reported the sequencing of about 900 cDNA tags from the M. truncatula root hairs. In addition, hundreds more expressed sequence tags (ESTs) have been isolated and characterized from effective root nodules of L. japonicus and M. truncatula, and a ...

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“…The exact function of these proteins is still unclear but numerous studies have demonstrated the induction of dehydrins by drought stress (Maitra and Cushman, 1994;Giordani et al, 1999;Thompson and Corlett, 1995). Dehydrins are normally synthesized in maturating seeds during their desiccation, and in vegetative tissues of plants treated with ABA or exposed to environmental stress factors that result in cellular dehydration.…”

Section: Protective Factors Of Macromoleculesmentioning

confidence: 99%

In silico analysis of ESTs from roots of Rangpur lime (Citrus limonia Osbeck) under water stress

et al. 2007

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CitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and proline-related synthase. Some sequences could not be classified by using FunCat and remained with an unknown function. A large number of sequences presented high similarities to annotated genes involved with cell energy, protein synthesis and cellular transport, suggesting that Rangpur lime may sustain active cell growth under stressed condition. The presence of membrane transporters and cell signaling components could be an indication of a coordinated morphological adaptation and biochemical response during drought, helping to explain the higher tolerance of this rootstock to water stress.

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Isolation and Characterization of a Drought-Induced Soybean cDNA Encoding a D95 Family Late-Embryogenesis-Abundant Protein

Abstract: We thank Dr. Glenn Galau for the kind g& of the cotton Leal4 cDNA clone and the Oklahoma State University Recombmant DNA/ Protein Resource Facilih for automated DNA seauence analvsis.

Cited by 54 publications

References 7 publications

Soybean physiology and gene expression during drought

Soybean physiology and gene expression during drought

Genome-Wide Identification of Nodule-Specific Transcripts in the Model Legume Medicago truncatula

In silico analysis of ESTs from roots of Rangpur lime (Citrus limonia Osbeck) under water stress

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