Isolation and Characterization of A Metal-resistant Pseudomonas Aeruginosa Strain

Abstract

The use of microorganisms to remove heavy metals from industrial effluent is an area of extensive research and development. Attempts have been made to isolate and characterize metal-resistant microorganisms from treated oil mill industry effluent wastewater samples. The metal-resistant organisms that showed values of minimum inhibitory concentration towards metals (Cd, Cr, Ni and Pb) ranging from 100 to 800 ppm level were screened. A potent metal-resistant organism, isolate BC15 from the wastewater samples was tentatively identified as Pseudomonas sp. Detailed analysis of morphological, biochemical and 16S rDNA sequence of the isolate revealed that it is closely related to Pseudomonas aeruginosa (94%). Pseudomonas BC15 was capable of absorbing 93% Ni, 65% Pb, 50% Cd and 30% Cr within 48 h from the medium containing 100 mg of each heavy metal per liter. The multiple metal tolerance of this strain was also associated with resistance to antibiotics such as ampicillin, tetracycline, chloramphenicol, erythromycin, kanamycin and streptomycin.

Cu-resistant Kocuria sp. CRB15: a potential PGPR isolated from the dry tailing of Rakha copper mine

“…The inability to grow at higher concentration was associated with reduction in growth due to the metal binding capacity of CRB15 which resulted by means of interaction between metal cations and functional groups of the cell wall. Similar results were also reported by Friis and Myers-Keith (1986) and Raja et al (2006). …”

“…Growth rates of the strain CRB15 in the presence of heavy metals were consistently slower than that of control. Similar results were reported by Suresh Kumar et al (2001), Pal et al (2004) and Raja et al (2006).…”

Phenotypic and molecular characterization of a novel isolate of Pseudomonas aeruginosa applicable for biosorption of Pb2+ from waste water: Application of biosorption isotherm models

“…on different concentrations of lead metal from 0.4 to 0.7g/l with consideration of incubation time and found that the growth increase (lag phase) until reach stationary phase at 360min. Growth pattern of P. aeruginosa strain BC15 on different concentrations of Cr, Ni, Pb and Cd metals was studied by Raja et al (2006). It was reported by different authors that the growth of the bacterial isolates is reduced as affected by the metals in higher concentrations comparing to the control (Suresh et al, 1998;Suresh et al, 2001 andPal et al, 2004).…”

Isolation and Screening of Rhizobacteria from Scirpus Grossus Plant after Lead (Pb) Exposure

“…The characterization of rhizobacteria colonies based on the shape and colors of the colonies were examined under a microscope (Leica-DM 1000) [15,16]. Chemical tests of of Gram stain, calatalase, oxidase and motility were also performed.…”