Isolation and Characterization of a Novel Tembusu Virus Circulating in Muscovy Ducks in South China

Zhang, Yan; Shen, Hao; Wang, Z.; Lin, Wencheng; Xie, Qingmei; Bi, Yingzuo; Chen, F.

doi:10.1111/tbed.12525

Cited by 18 publications

(6 citation statements)

References 6 publications

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“…The mature virion could be observed by transmission electron microscopy (TEM), having the nucleic acid in the center and an envelope with the diameter of 40 to 60 nm [9]. There is a high homology among various DTMUV strains and other Flaviviruses [10].…”

Section: Introductionmentioning

confidence: 99%

The dynamic distribution of duck Tembusu virus in the spleen of infected shelducks

et al. 2019

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Background Duck Tembusu virus (DTMUV) is a novel member of Flavivirus . The isolated and purified DTMUV strain XZ-2012 was used as a strain model, to intramuscularly inject the six-month egg-laying shelducks with the infective dose of 10 4 TCID 50 . The dynamic distribution of the virus in spleen at different time post-infection (pi) was studied using RT-PCR, RT-qPCR, ELISA, immunofluorescence and transmission electron microscopy (TEM). Result The results showed that the virus occurred in the spleen after 2 hpi and lasted up to 18 dpi. The registered viral load increased from 2 hpi to 3 dpi, and then it diminished from 6 dpi to 18 dpi with a slight rise at 12 dpi. From 2 hpi to 6 dpi the DTMUV particles were mostly distributed in the periellipsoidal lymphatic sheath (PELS) of spleen white pulp, few being found in the sheathed capillary. From 9 dpi to 18 dpi, the DTMUV particles were migrating into periarterial lymphatic sheaths (PALS) around the central artery through the red pulp. Under TEM, the virus particles could be observed mostly in lymphocytes and macrophages. Conclusion It was suggested that DTMUV invaded lymphocytes and macrophages of the spleen at 2 hpi and replicated significantly from 1 dpi to 3 dpi, being eliminated from 9 dpi to 18 dpi. This is the first study on the dynamic distribution of DTMUV from invasion to elimination in duck spleen conducted by molecular and morphological methods. It could provide theoretical basis for the occurrence, development and detoxification of the virus in the organs of the immune system.

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Section: Introductionmentioning

confidence: 99%

The dynamic distribution of duck Tembusu virus in the spleen of infected shelducks

et al. 2019

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“…TMUV was reported to cause epidemics affecting ducks in China in 2010, leading to huge economic losses and attracting widespread attention (Cao et al, 2011). A wide range of avian species, including geese, chickens, house sparrows, and pigeons, was confirmed to be naturally infected by TMUV (Tang et al, 2013a(Tang et al, , 2015Chen et al, 2014;Yan et al, 2017Yan et al, , 2022Zhu et al, 2022), and infections in human have been demonstrated by serological tests or the presence of RNA (Wolfe et al, 2001;Tang et al, 2013b). Due to the lack of widespread surveillance, the virus is mostly reported in Thailand, Malaysia, and China, with few reports of TMUV in Vietnam (Hamel et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Survey of mosquito species and mosquito-borne viruses in residential areas along the Sino–Vietnam border in Yunnan Province in China

Tian

He²,

Shang³

et al. 2023

Front. Microbiol.

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Mosquitoes are capable of carrying complex pathogens, and their feeding habits on the mammalian blood can easily mediate the spread of viruses. Surveillance of mosquito-based arbovirus enables the early prevention and control of mosquito-borne arboviral diseases. The climate and geography of Yunnan Province in China are ideal for mosquitoes. Yunnan shares borders with several other countries; therefore, there exists a high risk of international transmission of mosquito-mediated infectious diseases. Previous studies have focused more on the Sino–Laos and Sino–Myanmar borders. Therefore, we focused on the neighborhoods of Malipo and Funing counties in Wenshan Prefecture, Yunnan Province, China, which are located along the Sino–Vietnam border, to investigate the species of mosquitoes and mosquito-borne viruses in the residential areas of this region. This study collected 10,800 mosquitoes from 29 species of 8 genera and grouped to isolate mosquito-borne viruses. In total, 62 isolates were isolated and classified into 11 viral categories. We demonstrated a new distribution of mosquito-borne viruses among mosquitoes in border areas, including Tembusu and Getah viruses, which can cause animal outbreaks. In addition, Dak Nong and Sarawak viruses originating from Vietnam and Malaysia, respectively, were identified for the first time in China, highlighting the complexity of mosquito-borne viruses in the Sino–Vietnam border region. The awareness of the importance of viral surveillance and prevention measures in border areas should be further encouraged to prevent future outbreaks of potentially infectious diseases.

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“…Since TMUV was first reported in 2010, it had widely spread in China (Liu et al, 2013;Z. Xie et al, 2014;Yan et al, 2017;Yun et al, 2012;X. Zhou et al, 2016;Zhu et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

The spread of Tembusu virus in China from 2010 to 2019

Wang¹,

Cui²,

Li³

2020

Preprint

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Tembusu virus (TMUV) is a positive-sense RNA virus that is associated with severe reduction in egg production and even death in ducks. TMUV infection shows high incidence and is a threat to the global duck industry. However, the possible origin, genotype, and codon usage bias of TMUV are not very clear. Here, we addressed these questions by analyzing the available genomic sequences from China. The results showed that the ancestor of avian TMUV was most likely a mosquito TMUV. Moreover, three TMUV clades were identified by three different phylogenetic analysis methods. The TMUV genome exhibits a stronger mutation pressure than natural selection pressure. TMUV has the potential to cause an infection outbreak among humans, thereby posing a major threat to public health. Our findings provide important insights that reveal the ongoing TMUV spread in China and can aid in future prevention and control.

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Isolation and Characterization of a Novel Tembusu Virus Circulating in Muscovy Ducks in South China

Cited by 18 publications

References 6 publications

The dynamic distribution of duck Tembusu virus in the spleen of infected shelducks

The dynamic distribution of duck Tembusu virus in the spleen of infected shelducks

Survey of mosquito species and mosquito-borne viruses in residential areas along the Sino–Vietnam border in Yunnan Province in China

The spread of Tembusu virus in China from 2010 to 2019

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