Isolation and Characterization of a Novel Haloacid Permease from
            <i>Burkholderia cepacia</i>
            MBA4

Yu, Manda; Faan, Yun-Wing; Chung, Wilson Y. K.; Tsang, Jwh

doi:10.1128/aem.00576-07

Cited by 23 publications

(28 citation statements)

References 55 publications

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“…Although studies of Gordonia sp. KTR9 show that bacteria do not require a dedicated transporter for RDX uptake (13), bacterial genes encoding related functions are frequently situated close to each other (14,34,35). Therefore, we speculated that the aroP-encoded permease might also be involved in the transport of RDX.…”

Section: Discussionmentioning

confidence: 99%

“…MA1 encodes a putative permease transporter, termed AroP (6), which shares 72% identity with a putative amino acid permease from Mycobacterium vanbaalenii PYR-1 (NCBI accession number YP_952672.1). Two studies have documented permeases located close to genes with related function (14,15). The mode of RDX removal by R. rhodochrous 11Y is unknown, but given the proximity to xplA, it is possible that the aroP-encoded permease is involved in the transport of RDX or its breakdown products.…”

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confidence: 99%

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Analysis of the xplAB -Containing Gene Cluster Involved in the Bacterial Degradation of the Explosive Hexahydro-1,3,5-Trinitro-1,3,5-Triazine

Chong

Sabir

Lorenz

et al. 2014

Appl Environ Microbiol

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c Repeated use of the explosive compound hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) on military land has resulted in significant soil and groundwater pollution. Rates of degradation of RDX in the environment are low, and accumulated RDX, which the U.S. Environmental Protection Agency has determined is a possible human carcinogen, is now threatening drinking water supplies. RDX-degrading microorganisms have been isolated from RDX-contaminated land; however, despite the presence of these species in contaminated soils, RDX pollution persists. To further understand this problem, we studied RDX-degrading species belonging to four different genera (Rhodococcus, Microbacterium, Gordonia, and Williamsia) isolated from geographically distinct locations and established that the xplA and xplB (xplAB) genes, which encode a cytochrome P450 and a flavodoxin redox partner, respectively, are nearly identical in all these species. Together, the xplAB system catalyzes the reductive denitration of RDX and subsequent ring cleavage under aerobic and anaerobic conditions. In addition to xplAB, the Rhodococcus species studied here share a 14-kb region flanking xplAB; thus, it appears likely that the RDX-metabolizing ability was transferred as a genomic island within a transposable element. The conservation and transfer of xplAB-flanking genes suggest a role in RDX metabolism. We therefore independently knocked out genes within this cluster in the RDX-degrading species Rhodococcus rhodochrous 11Y. Analysis of the resulting mutants revealed that XplA is essential for RDX degradation and that XplB is not the sole contributor of reducing equivalents to XplA. While XplA expression is induced under nitrogen-limiting conditions and further enhanced by the presence of RDX, MarR is not regulated by RDX.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Analysis of the xplAB -Containing Gene Cluster Involved in the Bacterial Degradation of the Explosive Hexahydro-1,3,5-Trinitro-1,3,5-Triazine

Chong

Sabir

Lorenz

et al. 2014

Appl Environ Microbiol

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“…Slow growth was observed for D9 strain compared to Dw possibly due to inefficient substrate uptake system gene in D9 compared to Dw. Yu et al (2007) suggested the uptake of halogenated compound into the bacterial cell was due to haloacid permease. Previous study identified haloalkanoic permease uptake system gene in the dehalogenase producing bacteria was essential for substrate uptake as proposed by Jing et al (2010).…”

Section: Discussionmentioning

confidence: 99%

Molecular identification and characterization of Dalapon-2,2-dichloropropionate (2,2DCP)-degrading bacteria from a Rubber Estate Agricultural area

Wong¹,

Huyop²

2012

Afr. J. Microbiol. Res.

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The extensive use of herbicides in agricultural area over the past years resulted in environmental pollution. A total of 4 potential bacterial strains were isolated from the rubber estate using minimal media containing 2, 2-dichloropropionate (2, 2-DCP) as sole source of carbon and energy. The 16S rDNA analysis was carried out for genus identification study. Phylogenetic analysis suggested that strains D1, D6 and D9 were closely related with Enterobacter cloacae ATTC13047, whereas, strain Dw was closely related with Burkholderia sp. KU-25 with distance values of 0.001 and 0.004 base substitutions per site, respectively. Since strains D1, D6 and D9 belong to the same genus, therefore, D9 and Dw were further analysed. The growth profiles of both D9 and Dw in minimal liquid medium containing 40, 20 and 10 mM 2,2-DCP were studied. Strain Dw growth was approximately 3 times faster than D9. In conclusion, faster growth rate for strain Dw indicates the pragmatic application of the bacterial strains to degrade residual herbicide in the environment.

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“…The ability for MBA4 to utilize haloacids is conferred by a 2-haloacid dehalogenase Deh4a [1] which has been well characterized [9-11]. A haloacid permease gene, deh4p , which forms an operon with deh4a , was identified by means of chromosome walking [12]. The function of Deh4p was confirmed by heterologous expression in E. coli [13], and its topology determined with a PhoA-LacZ dual reporters system [14].…”

Section: Introductionmentioning

confidence: 99%

“…In the process of characterizing the MCA-uptake activity of MBA4, it was found that acetate was also recognized by the MCA-inducible uptake system [12,15]. Since acetate and MCA are structurally similar, it is reasonable to speculate that MCA was transported by an acetate-transport system.…”

Section: Introductionmentioning

confidence: 99%

Transports of acetate and haloacetate in Burkholderiaspecies MBA4 are operated by distinct systems

Kong

Tsang

2012

BMC Microbiol

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BackgroundAcetate is a commonly used substrate for biosynthesis while monochloroacetate is a structurally similar compound but toxic and inhibits cell metabolism by blocking the citric acid cycle. In Burkholderia species MBA4 haloacetate was utilized as a carbon and energy source for growth. The degradation of haloacid was mediated by the production of an inducible dehalogenase. Recent studies have identified the presence of a concomitantly induced haloacetate-uptake activity in MBA4. This uptake activity has also been found to transport acetate. Since acetate transporters are commonly found in bacteria it is likely that haloacetate was transported by such a system in MBA4.ResultsThe haloacetate-uptake activity of MBA4 was found to be induced by monochloroacetate (MCA) and monobromoacetate (MBA). While the acetate-uptake activity was also induced by MCA and MBA, other alkanoates: acetate, propionate and 2-monochloropropionate (2MCPA) were also inducers. Competing solute analysis showed that acetate and propionate interrupted the acetate- and MCA- induced acetate-uptake activities. While MCA, MBA, 2MCPA, and butyrate have no effect on acetate uptake they could significantly quenched the MCA-induced MCA-uptake activity. Transmembrane electrochemical potential was shown to be a driving force for both acetate- and MCA- transport systems.ConclusionsHere we showed that acetate- and MCA- uptake in Burkholderia species MBA4 are two transport systems that have different induction patterns and substrate specificities. It is envisaged that the shapes and the three dimensional structures of the solutes determine their recognition or exclusion by the two transport systems.

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Isolation and Characterization of a Novel Haloacid Permease from Burkholderia cepacia MBA4

Cited by 23 publications

References 55 publications

Analysis of the xplAB -Containing Gene Cluster Involved in the Bacterial Degradation of the Explosive Hexahydro-1,3,5-Trinitro-1,3,5-Triazine

Analysis of the xplAB -Containing Gene Cluster Involved in the Bacterial Degradation of the Explosive Hexahydro-1,3,5-Trinitro-1,3,5-Triazine

Molecular identification and characterization of Dalapon-2,2-dichloropropionate (2,2DCP)-degrading bacteria from a Rubber Estate Agricultural area

Transports of acetate and haloacetate in Burkholderiaspecies MBA4 are operated by distinct systems

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