Isolation and characterization of a repetitive DNA element from the genome of the human filarial parasite, Brugia malayi

Sex chromosome evolution in parasitic nematodes of humans

“…HhaI is in tandemly arrayed copies of a~322 bp monomer and has been successfully used as a real-time PCR target for detection of B. malayi infections from patient blood 37,38 . MboI is less conserved, with monomers that show considerable variation in sequence and length 39 . The HhaI tandem DNA repeats were previously thought to constitute close to 12% of the genome 40 , but the initial assembly of the genome 6 as well as our current assembly only identifies HhaI as 1.6% of the genome, with a read-based analysis of sequencing data from 22 males suggesting 2.6 ± 1.6%.…”

“…In contrast, MboI repeats were overrepresented on the putative chromosome Y contigs (Fisher's Exact Test, p-value < 0.00001) (Supplementary Data 4, Supplementary Fig. 5b) with 21% of the MboI sequence on these contigs being attributed to BmMbo8 39 , considered the prototypic member of this family of repeats. In fact, 90% of the BmMbo8 sequences were found specifically on the putative chromosome Y contigs, while the remaining 10% are located on one end of NB as well as the small, unplaced contigs not attributed to chromosome Y or haplotypes.…”