Isolation and characterization of a Pseudomonas sp. strain IITR01 capable of degrading α-endosulfan and endosulfan sulfate

Bajaj, Abhay; Pathak, Ashutosh; Mudiam, Mohana Krishna Reddy; Mayilraj, Shanmugam; Manickam, Natesan

doi:10.1111/j.1365-2672.2010.04845.x

Cited by 50 publications

(23 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Formation of these metabolites during a-endosulfan degradation has also been reported previously (Weir et al 2006;Bajaj et al 2010;Jesitha et al 2015). Bajaj et al (2010) reported that after 9 days of incubation; Pseudomonas sp. strain IITR01 was able to degrade 86% of a-endosulfan.…”

Section: Formation Of A-endosulfan Metabolites By the Culturessupporting

confidence: 80%

Biodegradation of α-endosulfan via hydrolysis pathway by Stenotrophomonas maltophilia OG2

et al. 2017

View full text Add to dashboard Cite

Stenotrophomonas maltophilia OG2 was isolated from the intestine of cockroaches that was collected from a cow barn contaminated some pesticides belong to pyrethroid and organochlorine groups. OG2 was able to degrade a-endosulfan in non sulfur medium (NSM) as a sole sulfur source for growth within 10 days of incubation. The effects of some growth parameters on endosulfan biodegradation by OG2 was studied and found that the biodegradation was significantly affected by the endosulfan concentrations, pH and temperature. Experimental results obtained in different conditions show that the optimum concentration of a-endosulfan, pH and temperature were 100 mg/L, 8.0 and 30°C, respectively. Under these conditions, the bacterium degraded 81.53% of the a-endosulfan after 10 days. The concentration of a-endosulfan and its metabolites was determined by HPLC. Endosulfan ether, endosulfan lactone and endosulfan diol were the main metabolites in culture, but did not produce toxic metabolite, endosulfan sulfate. These results suggested that S. maltophilia OG2 degrades a-endosulfan via a hydrolysis pathway. The present study indicates that strain OG2 may have potential use in the biodegradation of pesticides contaminated environments.

show abstract

Section: Formation Of A-endosulfan Metabolites By the Culturessupporting

confidence: 80%

Biodegradation of α-endosulfan via hydrolysis pathway by Stenotrophomonas maltophilia OG2

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Bajaj et al [10] isolated and identified Pseudomonas sp. strain IITR01, which has the ability to degrade alfa-endosulfan.…”

Section: Discussionmentioning

confidence: 99%

“…In their study, the appropriate amount of lactone was prepared. The GC/MS analysis revealed degradation of alfa-endosulfan and endosulfan sulfate as well as production of endosulfan diol, endosulfan ether, and endosulfan lactone [10] .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and Identification of Endosulfan-Degrading Bacteria and Evaluation of Their Bioremediation in Kor River, Iran

Kafilzadeh

Ebrahimnezhad

Tahery

2015

Osong Public Health and Research Perspectives

View full text Add to dashboard Cite

ObjectivesEndosulfan is a lipophilic insecticide, which causes severe health issues due to its environmental stability, toxicity, and biological reservation in organisms. It is found in the atmosphere, soil, sediments, surface waters, rain, and food in almost equal proportions. The aim of this study was to isolate and identify endosulfan-degrading bacteria from the Kor River and evaluate the possibility of applying bioremediation in reducing environmental pollution in the desired region.MethodsSamples of surface sediments and water were collected from three different stations in two seasons (summer and autumn), as these are areas with high agricultural activity. Isolated bacteria were identified by various biochemical tests and morphological characteristics. The amounts of degradation of endosulfan isomers and metabolites produced as a result of biodegradation were then analyzed using gas chromatography/mass spectrometry.ResultsIn this study, the following five bacterial genera were able to degrade endosulfan: Klebsiella, Acinetobacter, Alcaligenes, Flavobacterium, and Bacillus. During biodegradation, metabolites of endosulfan diol, endosulfan lactone, and endosulfan ether were also produced, but these had lesser toxicity compared with the original compound (i.e., endosulfan).ConclusionThe five genera isolated can be used as a biocatalyst for bioremediation of endosulfan.

show abstract

“…, and Pseudomonas sp. (Singh and Singh 2011; Bajaj et al 2010; Goswami et al 2009; Hussain et al 2007a; Kumar and Philip 2007; Lee et al 2006; Weir et al 2006; Kwon et al 2002; Sutherland et al 2002). …”

Section: Introductionmentioning

confidence: 99%

Penicillium sp. as an organism that degrades endosulfan and reduces its genotoxic effects

et al. 2014

View full text Add to dashboard Cite

Endosulfan is an organochloride and persistent pesticide that has caused concern because of its impact in the environment and its toxicity to and bioaccumulation in living organisms. In this study, we isolated an endosulfan-degrading fungus from the activated sludge from an industrial wastewater treatment plant. Through repetitive enrichment and successive subculture in media containing endosulfan as the sole carbon source, a fungus designated CHE 23 was isolated. Based on a phylogenetic analysis, strain CHE 23 was assigned to the genus Penicillium sp. In a mineral salt medium with 50 mg/l endosulfan as the sole source carbon, CHE 23 removed the added endosulfan in a period of six days. To verify the decrease in endosulfan toxicity due to the activity of the fungus, we performed genotoxicity tests trough the single cell gel electrophoresis assay or comet assay, with Eisenia fetida as the bioindicator species. This organism was exposed to the supernatants of the culture of the fungus and endosulfan. Our results indicated that the genotoxicity of endosulfan was completely reduced due the activity of this fungus. These results suggest that the Penicillium sp. CHE 23 strain can be used to degrade endosulfan residues and/or for water and soil bioremediation processes without causing toxicity problems, which are probably due to the generation of no-toxic metabolites during biodegradation.

show abstract

Isolation and characterization of a Pseudomonas sp. strain IITR01 capable of degrading α-endosulfan and endosulfan sulfate

Cited by 50 publications

References 35 publications

Biodegradation of α-endosulfan via hydrolysis pathway by Stenotrophomonas maltophilia OG2

Biodegradation of α-endosulfan via hydrolysis pathway by Stenotrophomonas maltophilia OG2

Isolation and Identification of Endosulfan-Degrading Bacteria and Evaluation of Their Bioremediation in Kor River, Iran

Penicillium sp. as an organism that degrades endosulfan and reduces its genotoxic effects

Contact Info

Product

Resources

About