Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali

Kassogue, Amadou; Maiga, Kadia; Traoré, Diakaridia; Dıcko, Amadou; Fane, Rokiatou; Guissou, Tiby; Faradji, Fatouma Alhadji; Valicente, Fernando Hercos; Hamadoun, Abdoulaye

doi:10.5897/ajar2015.9848

Cited by 6 publications

(4 citation statements)

References 21 publications

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“…Bacillus thuringiensis BtD5, Bacillus thuringiensis BtI4' and Bacillus thuringiensis BtI4″ in this study was isolated from Malian agricultural soils [2], and maintained in the LaboREM-Biotech, Faculty of Sciences and Techniques, bacterial culture collection in Mali. These bacteria, selected for their high insect pest biocontrol capacity, was assessed for their Plant Growth Promotion (PGP) activities.…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…iron through siderophores synthesis); parasitism, that may involve production of hydrolytic enzymes such as chitinase, β-1,3glucanase, protease and cellulose, that lyse pathogen cell walls, inhibit the pathogens by secreting anti-microbial compounds and induce systemic resistance in host plants [1]. There are several species of Bacillus known as plant growth and health supporting in nature because of beneficial characteristic features which act directly and indirectly [2,3]. The principal mechanisms of plant growth promotion include: production of phytohormones such as indole acetic acid (IAA), solubilization of phosphate, siderophore production, antibiosis, inhibition of plant ethylene synthesis, production of volatile compounds such as HCN and induction of plant systemic resistance to pathogens [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have revealed that these bacteria could promote plant growth and protect plants against important crop microbial pathogens [9]. The present study was designed to screen of the multiple plant growth promoting activities of some Bacillus thuringiensis strains isolated from Malian Agricultural soils and selected for their high insecticidal activities against maize and rice insect pests [2,10] and evaluate their ability to improve maize seed germination and seedling vigor in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Bacillus thuringiensis Strains Isolated from Agricultural Soils in Mali Tested for Their Potentiality on Plant Growth Promoting Traits

Kassogue

Dıcko

Traoré

et al. 2016

BMRJ

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Aims: To screen of the multiple plant growth promoting activities of some Bacillus thuringiensis strains isolated from Malian Agricultural soils and evaluate their ability to improve maize seed germination and seedling vigor in vitro. Study Design: Strains of Bacillus thuringiensis (Bt) used in this study belong to collection of the Laboratory of Research in Microbiology and Microbial Biotechnology (LaboREM-Biotech) isolated from different agricultural soils of Mali. Methodology: Different tests, namely: Phosphate solubilization, Siderophore production, Indol acetic acid cellulase and chitinase production tests were performed to confirm the PGP characteristic of the insecticidal B. thuringiensis strains screened. In vitro test was performed in the laboratory to confirm the capacity of these bacteria to enhance maize germination and seedling vigor. Results: All tested Bacillus strains solubilize efficiently insoluble phosphate, but BtI4″ showed the Original Research Articlehighest clearance zone around its colony. In this study, except in BtI4', the siderophore production was significantly elevated in the other Bt strains tested. Only BtD5 was able to produce Indol acetic acid. Contrary, except BtD5, all the isolates produce chitinase and cellulase. Exept IAA, the isolate BtI4″ produce all the tested compounds and showed the highest % seed germination and seedling vigor. Conclusion:In the current study, plant growth promotion analysis of three B. thuringiensis strains from Malian agricultural soils were assessed in relation with maize seed germination and seedling vigor. These tested Bt strains showed several plant growth-promoting characteristics. These activities may allow the use of these isolates for plant growth promotion. Future work will address the applications of the selected bacteria in biocontrol and plant growth promotion. Insect pest biocontrol, enhancement of plant nutrition and production of phytohormon are the mechanisms involved.

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Section: Bacterial Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bacillus thuringiensis Strains Isolated from Agricultural Soils in Mali Tested for Their Potentiality on Plant Growth Promoting Traits

Kassogue

Dıcko

Traoré

et al. 2016

BMRJ

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“…However, the average rice productivity in Mali (3.38 t/ha) is below world average (4.40 t/ha) and accounts one third of the rice productivity in Australia [2]. Low rice yields are attributed mainly to soils deficiencies and diseases [3][4][5] Soil P and N deficiencies was observed as the main constraints in yield decreases in Africa, mainly in Mali [6,4]. To increase production in a sustainable way, one of the biological approaches of achieving this objective is through the use of P-solubilizing bacteria (PSB) [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Endophytic Bacteria-based Biostimulant Improved Rice (Oryzae sativa L.) Growth and Production in Mali

Nantoumé,

Kassogué,

Dao

et al. 2023

SAJRM

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Aims: Rice (Oryza sativa L.) is usually the staple food of more than half of the world's population, and in particular that of Mali. Mali is among the largest producers of rice in West Africa. But also given the in-population growth and in consumption, Mali is forced to resort to imports to cover its rice needs, thus 45% of the rice sold on the national market comes from imports. For self-sufficiency in rice, the aim of this research is to formulate a biostimulant with high capacity to improve rice growth and production in Mali. Study Design: This experimental research involving a Malian local rice cultivar named Kogoni and biostimulant endophyte bacteria isolated from rice in Mali. Pace and Duration of Study: This research was carried out in greenhouse at LaboREM-Biotech and in the field at the Office of irrigated perimeters of Baguineda (OPIB) for 3 years. Study Design: To achieve this objective: endophyte bacteria with high plant growth promotion activities were selected from bacterial collection of LaboREM, biostimulants composed with selected endophytes were formulated, formulated biostimulants were tested in greenhouses and fields to prove their capacities to improve rice growth and production. Results: A total of five isolates, including 3 from Xoo and 2 from Xoc, were isolated in Niono from two varieties of rice: Kogoni91-1 and Adny11. No isolate characteristic of Xanthomonas oryzae was observed on samples from Baguineda. The three selected rice endophytes were tested in vitro to assess their effectiveness in controlling Xoo and Xoc. Following this test, 100% of the endophytes showed significant antimicrobial activity against Xoo and Xoc with an inhibition diameter varying between 6 and 28.5 mm. endo Ad9 was selected for its strong ability to inhibit the growth of both pathogens at the same time.

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Isolation, characterization, and bio-insecticidal efficiency of Ethiopian isolates of Bacillus thuringiensis against Galleria mellonella L. (Lepidoptera: Pyralidae) and tomato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae)

Gebremariam

Chekol

Assefa

2021

Egypt J Biol Pest Control

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Background Bacillus thuringiensis is a Gram-positive, rod-shaped, and spore-forming microbial entomopathogen. The silverleaf whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), is a worldwide important cryptic species causing serious economic damage to several vegetable crops including tomato. The aim of this study was to characterize and evaluate the bio-insecticidal effects of locally isolated Bacillus thuringiensis (Bt) against Galleria mellonella and Bemisia tabaci. Results Thirty-one isolates of Bt were obtained from 70 soil samples based on cultural characterization coupled with insecticidal crystal protein detection. All of the 31 bacterial isolates were pre-screened for their bio-insecticidal property against the greater wax moth, Galleria mellonella L. larvae. Only 20 (64.5%) isolates were virulent to G. mellonella with a percentage mortality that ranged from 2.50 to 95%. The groEL gene sequences of all 20 entomopathogenic indigenous isolates displayed 99–100% similarity with Bt isolates. Bioassay evaluation of 12 selected isolates against 3rd to 4th nymphal instars of B. tabaci with spore-crystal suspensions of 1 × 109 spores/ml caused 0 to 31.25%, 0 to 57%, and 0 to 82.5% percentage mortality within 48, 96, and 144 h accordingly. Among isolates of Bt, isolate AAUDS-16 had the highest virulence, followed by the isolate AAUES-69D with LC50 values of 9.67 × 106 spores/ml and 1.16 × 107 spores/ml, respectively. The virulent isolates were tested for their growth response to a different temperature range between 15 and 40 °C. All isolates showed a maximum growth rate around 30 °C. Conclusions Bt isolates of AAUES-69D and AAUDS-16 displayed high insecticidal potential against B. tabaci nymphs and G. mellonella larvae, and showed a maximum growth rate in a wide range of temperature. High virulence and temperature-tolerant isolates should be important candidates for diverse insecticidal toxin studies.

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Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali

Cited by 6 publications

References 21 publications

Bacillus thuringiensis Strains Isolated from Agricultural Soils in Mali Tested for Their Potentiality on Plant Growth Promoting Traits

Bacillus thuringiensis Strains Isolated from Agricultural Soils in Mali Tested for Their Potentiality on Plant Growth Promoting Traits

Endophytic Bacteria-based Biostimulant Improved Rice (Oryzae sativa L.) Growth and Production in Mali

Isolation, characterization, and bio-insecticidal efficiency of Ethiopian isolates of Bacillus thuringiensis against Galleria mellonella L. (Lepidoptera: Pyralidae) and tomato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae)

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