Isolation and characterization of bacteria from two soil samples and their effect on wheat (Triticum aestivum L.) growth promotion

Paul, Gobindo Kumar; Mahmud, Shafi; Naher, Kamrun; Jabin, Tabassum; Mahmud, Liton; Haque, M. N.; Uddin, Nesar; Zaman, Shahriar; Saleh, Saleh

doi:10.5455/jabet.2020.d132

Cited by 6 publications

(2 citation statements)

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“…PGPR also reduces the use of chemical fertilizer by engaging in growth simulation. T. aestivum seeds germinate and grow faster after receiving beneficial bacterial inoculation 27 .…”

Section: Discussionmentioning

confidence: 99%

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Volatile compounds of Bacillus pseudomycoides induce growth and drought tolerance in wheat (Triticum aestivum L.)

Paul

Mahmud

Dutta

et al. 2022

Sci Rep

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The plant growth-boosting biofilm-forming bacteria Bacillus pseudomycoides is able to promote growth and drought stress tolerance in wheat by suppressing the MYB gene, which synthesizes Myb protein (TaMpc1-D4) through secreted volatile compounds. In the present study, Triticum aestivum seeds were inoculated with five distinct bacterial strains. The growth, germination rate, root-shoot length, RWC, and chlorophyll content of seedlings were investigated. Furthermore, the levels of soluble sugars, proteins, H2O2, NO, cell death, and antioxidant enzymes (CAT, SOD, POD, and APX) were observed throughout the growth stage. All of the results showed that B. pseudomycoides had a substantially higher ability to form biofilm and promote these traits than the other strains. In terms of molecular gene expression, B. pseudomycoides inoculation strongly expressed the Dreb1 gene by silencing the expression of MYB gene through secreted volatile compounds. For identifying the specific volatile compound that silenced the MYB gene, molecular docking with Myb protein was performed. Out of 45 volatile compounds found, 2,6-ditert-butylcyclohexa-2,5-diene-1,4-dione and 3,5-ditert-butylphenol had a binding free energy of − 6.2 and − 6.5, Kcal/mol, respectively, which predicted that these compounds could suppress this protein's expression. In molecular dynamics simulations, the RMSD, SASA, Rg, RMSF, and hydrogen bonding values found assured the docked complexes' binding stability. These findings suggest that these targeted compounds may be suppressing Myb protein expression as well as the expression of Dreb1 and other drought response genes in wheat. More research (field trial) into plant growth and drought stress is needed to support the findings of this study.

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“…PGPR also reduces the use of chemical fertilizer by engaging in growth simulation. T. aestivum seeds germinate and grow faster after receiving beneficial bacterial inoculation 27 .…”

Section: Discussionmentioning

confidence: 99%

“…Seeds were inoculated with freshly cultured bacterium stains according to the method described by Paul, et al 2020 27 . Briefly, seeds were soaked and washed with distilled water for 20 min and then were sterilized with 70% (v/v) ethanol for 2.5 min.…”

Section: Methodsmentioning

confidence: 99%