Isolation and Characterization of Bacteria with Biochemical and Pharmacological Importance from Soil Samples of Dhaka City

Begum, Khadiza; Mannan, Sultana Juhara; Rezwan, Refaya; Rahman, Mahinur; Rahman, Shajidur; Kamal, Alam Nur E

doi:10.3329/dujps.v16i1.33390

Cited by 18 publications

(15 citation statements)

References 15 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this research, bacteria that produce extracellular enzymes (amylase, cellulase and protease) were isolated from soil samples. Begum et al [17] reported that soil bacteria have potential of being unique and exploited for commercial and industrial use. Production of protease by Bacillus, Enterobacter, Klebsiella and Staphylococcus observed in this study has been reported [18][19][20].…”

Section: Discussionmentioning

confidence: 99%

Isolation of Bacteria with Potential of Producing Extracellular Enzymes (Amylase, Cellulase and Protease) from Soil Samples

Fasiku

Ogunsola

Fakunle

et al. 2020

JAMB

View full text Add to dashboard Cite

Introduction: Amylase, cellulase and protease are known for hydrolyzing starch, cellulose and protein respectively and these enzymes can be produced by microorganisms. A single bacterium with potential of producing amylase, cellulase and protease will be an organism of high industrial value. Aims: This work aimed at isolating bacteria that will be able to produce three extracellular enzymes (amylase, cellulase and protease). Methodology: Soil samples were collected from eight different locations within Ajayi Crowther University, Oyo Town, Nigeria. Bacteria were isolated from these soil samples and were identified using morphological and biochemical characteristics. Isolated bacteria were screened for their ability to produce amylase, cellulase and protease on plate and enzymes’ relative activities on plate were determined. Results: Forty two bacteria were isolated from soil samples and identified to belong to the Genera Bacillus (30), Enterobacter (6), Klebsiella (3) and Staphylococcus (3). Eight (19%), Eleven (26%) and Nineteen (45%) out of 42 isolated bacteria were able to produce amylase, cellulase and protease on plate with relative activities ranging from 1.25 – 2.88, 1.39 – 4.50 and 1.13 – 5.17 respectively. All the eight amylolytic isolates (Bacillus species (5) and Enterobacter species (3)) were able to produce the three enzymes (amylase, cellulase and protease). Conclusion: Eight bacteria with ability to produce three enzymes (amylase, cellulase and protease) were isolated from soil samples and could be further employed in enzyme-producing industries.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation of Bacteria with Potential of Producing Extracellular Enzymes (Amylase, Cellulase and Protease) from Soil Samples

Fasiku

Ogunsola

Fakunle

et al. 2020

JAMB

View full text Add to dashboard Cite

show abstract

“…The bacterial isolate (K1) was identified on the basis of cell morphology and biochemical tests (Begum et al, 2017). Biochemical tests were performed using VITEK ® 2 system (bioMérieux, France) through colorimetric identification.…”

Section: Methodsmentioning

confidence: 99%

“…Kirby-Bauer disc diffusion method was adopted to evaluate the antibiotic sensitivity pattern of Staphylococcus aureus K1 against selected antibiotics (CLSI, 2013). Commercially available antibiotic discs (Oxoid™ UK) were used in this study including ceftriaxone (30 µg), ampicillin (10 µg), amoxicillin (30 µg), meropenem (10 µg), imipenem (10 µg), sulphamethoxazole/trimethoprim (25 µg), nalidixic acid (30 µg), cefoxitin (30 µg), ciprofloxacin (5 µg), ofloxacin (5 µg), ceftazidime (30 µg), aztreonam (30 µg), gentamycin (10 µg), vancomycin (30 µg), ertrapenem (10 µg), amikacin (30 µg), piperacillin/tazobactam (110 µg) and cefepime (30 µg) (Begum et al, 2017). The tests were conducted following manufacturer instructions.…”

Section: Methodsmentioning

confidence: 99%

Isolation and molecular characterization of the indigenous Staphylococcus aureus strain K1 with the ability to reduce hexavalent chromium for its application in bioremediation of metal-contaminated sites

Tariq

Waseem

Rasool

et al. 2019

PeerJ

View full text Add to dashboard Cite

Background Urbanization and industrialization are the main anthropogenic activities that are adding toxic heavy metals to the environment. Among these, chromium (in hexavalent: Cr+6 and/or trivalent Cr+3) is being released abundantly in wastewater due to its uses in different industrial processes. It becomes highly mutagenic and carcinogenic once it enters the cell through sulfate uptake pathways after interacting with cellular proteins and nucleic acids. However, Cr+6 can be bio-converted into more stable, less toxic and insoluble trivalent chromium using microbes. Hence in this study, we have made efforts to utilize chromium tolerant bacteria for bio-reduction of Cr+6 to Cr+3. Methods Bacterial isolate, K1, from metal contaminated industrial effluent from Kala Shah Kaku-Lahore Pakistan, which tolerated up to 22 mM of Cr6+ was evaluated for chromate reduction. It was further characterized biochemically and molecularly by VITEK®2 system and 16S rRNA gene sequencing respectively. Other factors affecting the reduction of chromium such as initial chromate ion concentration, pH, temperature, contact-time were also investigated. The role of cellular surface in sorption of Cr6+ ion was analyzed by FTIR spectroscopy. Results Both biochemical and phylogenetic analyses confirmed that strain K1 was Staphylococcusaureus that could reduce 99% of Cr6+ in 24 hours at 35 °C (pH = 8.0; initial Cr6+ concentration = 100 mg/L). FTIR results assumed that carboxyl, amino and phosphate groups of cell wall were involved in complexation with chromium. Our results suggested that Staphylococcusaureus K1 could be a promising gram-positive bacterium that might be utilized to remove chromium from metal polluted environments.

show abstract

“…Then the isolated bacterias were applied and mixed slowly by using an ose needle. Then, the positive results are marked by the formation of air bubbles in the surrounding colonies (Begum et al, 2017).…”

Section: Catalase Testmentioning

confidence: 99%

“…Stubbing to the butt and streaking the slant, it was incubated at 37 o C for 24 hours. Positive reactions are characterized by the presence of black precipitate on media (Brown, 2010;Begum et al, 2017).…”

Section: H 2 S Testmentioning

confidence: 99%

The Isolation and Identification Bacteria on Jallalah Animal (Study on the Feeding Tilapia (Oreochromis niloticus) with Chicken Manure As Foods)

Kurahman

Yuliawati

Haerunnisa

et al. 2020

EKW

View full text Add to dashboard Cite

In the hadith, Rasulullah SAW prohibit human to consume meat and milk from manure-eating animal (Jallalah animal). In this study, tilapia fishes were fed chicken manure (being Jallalah). Then, it was analyzed based on the microbe in their intestine. The purposes of this study are to isolate and to identify the types of bacteria, and to determine the pathogenic and non-pathogenic bacteria in the intestines of fish that have been fed chicken manure. Bacteria samples were isolated from the intestine of fish with chicken manure and the commercial fish food as a control. Bacteria identification was done by using morphological characterization, macroscopic and microscopic identification, and biochemical test. The results show that 21 isolated bacteria in the fish intestine that was fed chicken manure and six of the bacteria are pathogenic. Based on this study, it can be concluded that tilapia fish by feeding with chicken manure contains many types of pathogens bacteria. Briefly, tilapia fish is not good for being consumption.Abstrak: Dalam hadis yang diriwayatkan oleh Ibnu Umar, Rasulullah SAW. Melarang memakan daging dan meminum susu hewan pemakan kotoran (hewan Jallalah). Pada penelitian ini, ikan nila diberi pakan kotoran ayam (menjadi Jallalah). Selanjutnya ikan tersebut dianalisis kandungan mikroba yang terkandung didalam saluran pencernaannya. Tujuan penelitian ini adalah mengisolasi dan mengidentifikasi bentuk bakteri dan mendeterminasi bakteri patogen dan non-patogen yang terdapat didalam saluran pencernaan ikan ynag telah diberi pakan kotoran ayam. Sampel bakteri diisolasi dari saluran pencernaan ikan setelah diberi kotoran ayam dan ikan yang diberi pakan komersil (kontrol). Identifikasi bakteri dilakukan secara makroskopik, mikroskopik, dan uji biokimia. Hasil penelitian menunjukkan ditemukan 21 isolat bakteri dalam usus ikan yang diberi korotan ayam yang tergolong ke dalam genus Listeria, Staphylococcus, Alcaligenes, Cromobacterium, Edwardisiella, Micrococcus, Bacillus, dan Kurthia. Dari 21 jenis tersebut terdapat 6 jenis bakteri pathogen yaitu Edwarsiella sp, Micrococcus sp, Staphylococcus sp, Alcaligenes sp, Listeria sp dan Chromobacterium. Kesimpulan pada penelitian ini bahwa hewan ikan yang memakan kotoran ayam mengandung banyak jenis bakteri patogen sehingga tidak baik untuk di konsumsi.

show abstract

Isolation and Characterization of Bacteria with Biochemical and Pharmacological Importance from Soil Samples of Dhaka City

Cited by 18 publications

References 15 publications

Isolation of Bacteria with Potential of Producing Extracellular Enzymes (Amylase, Cellulase and Protease) from Soil Samples

Isolation of Bacteria with Potential of Producing Extracellular Enzymes (Amylase, Cellulase and Protease) from Soil Samples

Isolation and molecular characterization of the indigenous Staphylococcus aureus strain K1 with the ability to reduce hexavalent chromium for its application in bioremediation of metal-contaminated sites

The Isolation and Identification Bacteria on Jallalah Animal (Study on the Feeding Tilapia (Oreochromis niloticus) with Chicken Manure As Foods)

Contact Info

Product

Resources

About