Isolation and Characterization of Bacteriophages Against Pseudomonas syringae pv. actinidiae Causing Bacterial Canker Disease in Kiwifruit

Yu, Ji Gang; Lim, Jeong Ae; Song, Yu; Heu, Sung Gi; Kim, Gyoung Hee; Koh, Young Jin; Oh, Chang‐Sik

doi:10.4014/jmb.1509.09012

Cited by 55 publications

(41 citation statements)

References 27 publications

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“…actinidae which causes canker disease in kiwi fruit, and Pseudomonas syringae pv. tomato , which causes bacterial speck on tomatoes (Peitl et al 2017 ; Yu et al 2016 ).…”

Section: Indirect Mechanismsmentioning

confidence: 99%

Mechanisms of action of plant growth promoting bacteria

Olanrewaju

Glick

Babalola

2017

World J Microbiol Biotechnol

832

470

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The idea of eliminating the use of fertilizers which are sometimes environmentally unsafe is slowly becoming a reality because of the emergence of microorganisms that can serve the same purpose or even do better. Depletion of soil nutrients through leaching into the waterways and causing contamination are some of the negative effects of these chemical fertilizers that prompted the need for suitable alternatives. This brings us to the idea of using microbes that can be developed for use as biological fertilizers (biofertilizers). They are environmentally friendly as they are natural living organisms. They increase crop yield and production and, in addition, in developing countries, they are less expensive compared to chemical fertilizers. These biofertilizers are typically called plant growth-promoting bacteria (PGPB). In addition to PGPB, some fungi have also been demonstrated to promote plant growth. Apart from improving crop yields, some biofertilizers also control various plant pathogens. The objective of worldwide sustainable agriculture is much more likely to be achieved through the widespread use of biofertilizers rather than chemically synthesized fertilizers. However, to realize this objective it is essential that the many mechanisms employed by PGPB first be thoroughly understood thereby allowing workers to fully harness the potentials of these microbes. The present state of our knowledge regarding the fundamental mechanisms employed by PGPB is discussed herein.

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“…actinidae which causes canker disease in kiwi fruit, and Pseudomonas syringae pv. tomato , which causes bacterial speck on tomatoes (Peitl et al 2017 ; Yu et al 2016 ).…”

Section: Indirect Mechanismsmentioning

confidence: 99%

Mechanisms of action of plant growth promoting bacteria

Olanrewaju

Glick

Babalola

2017

World J Microbiol Biotechnol

832

470

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“…However, there have been phages isolated against the phytopathogen Pseudomonas syringae pv. actinidiae which can tolerate extended UV-B doses (Yu et al, 2015). Other potential factors that could cause phage decline on the phyllosphere are desiccation, temperature, pH as well as certain chemicals produced by plants (Erskine, 1973; Delitheos et al, 1997; Iriarte et al, 2007).…”

Section: Phage In the Phyllospherementioning

confidence: 99%

Bacteriophages and Bacterial Plant Diseases

et al. 2017

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Losses in crop yields due to disease need to be reduced in order to meet increasing global food demands associated with growth in the human population. There is a well-recognized need to develop new environmentally friendly control strategies to combat bacterial crop disease. Current control measures involving the use of traditional chemicals or antibiotics are losing their efficacy due to the natural development of bacterial resistance to these agents. In addition, there is an increasing awareness that their use is environmentally unfriendly. Bacteriophages, the viruses of bacteria, have received increased research interest in recent years as a realistic environmentally friendly means of controlling bacterial diseases. Their use presents a viable control measure for a number of destructive bacterial crop diseases, with some phage-based products already becoming available on the market. Phage biocontrol possesses advantages over chemical controls in that tailor-made phage cocktails can be adapted to target specific disease-causing bacteria. Unlike chemical control measures, phage mixtures can be easily adapted for bacterial resistance which may develop over time. In this review, we will examine the progress and challenges for phage-based disease biocontrol in food crops.

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“…The incubated samples were treated with chloroform (1% of the final volume) for 30 min, centrifuged at 3,000 g, 4°C for 15 min, and filtered with a 0.22 μm pore size filter. The presence of bacteriophages in the supernatant was confirmed with four E. amylovora strains and two E. pyrifoliae strains using a dotting assay ( Kropinski et al, 2009 ; Yu et al, 2016 ). Then, in order to isolate individual bacteriophages, the overlay assay ( Yu et al, 2016 ) was performed, and plaques with different sizes and shapes were picked separately.…”

mentioning

confidence: 97%

“…The presence of bacteriophages in the supernatant was confirmed with four E. amylovora strains and two E. pyrifoliae strains using a dotting assay ( Kropinski et al, 2009 ; Yu et al, 2016 ). Then, in order to isolate individual bacteriophages, the overlay assay ( Yu et al, 2016 ) was performed, and plaques with different sizes and shapes were picked separately. A total of 21 individual bacteriophages were picked based on their plaque sizes and shapes ( Fig.…”

mentioning

confidence: 97%