ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS IN MEDICINAL PLANT Centella asiatica AND STUDY THE EFFECTS ON FUNGAL ACTIVITIES

Abstract

Preparation of plant crude methanol extract Centella asiatica were purchased from the local retail market. All plant materials washed thoroughly under running tap water and air dried at room temperature for two days and ground into a fine powder. The air-dried and powdered plant material are extracted with 400 ml of 95.5% methanol, kept in the room temperature for 72 h with periodic stirring. Thereafter the extract was filtered by Medicinal plants are sources of important therapeutic aids for alleviating human ailments. The present research investigation was carried out to study anti-fungal activity of C. asiatica were tested against C. albicans, Aspergillus niger, and Penicillium sp. using two methods, disc diffusion method and broth dilution method. C. asiatica crude methanol extract was found to be the most effective against fungal activity. Compared to disc diffusion, broth dilution was a more appropriate method to quantitatively determine the anti-Candida activity of plant extract, whereby the MIC values of the crude extracts was determined. The result from disc diffusion assay demonstrates that plant extracts have an inhibitory effect. However, the broth dilution method result reveal that C. asiatica crude methanol extract has lower MIC values, meaning it has more prove that C. asiatica has an anti-fungal effect. Bioactive analysis results reveled that bioactive compounds present in the leaf, stems, roots, and the whole plant extract from Centella asiatica are the major chemical constituents are n-Hexadecanoic acid (99%), cis-Vaccenic acid (91%), 5-Hydroxymethylfurfural (88%), Tetradecanoic acid (86%). Further study is required to find out the specific phytochemical which is responsible for its medicinal value. ARTICLE INFO

Phytochemicals, Antioxidant and Antimicrobial Activity of Centella Asiatica Extracted by Solvent-Free Microwave Extraction

“…Therefore, the presence of rutin (flavonoid) and oleic acid in the extract promotes the production of antioxidants [22]. In this study, hexadecenoic acid was the second-highest compound found in C. asiatica, in which Bhuyar et al [17] also reported the presence of hexadecanoic acid (99 %) in C. asiatica extract, and the compound is known to be antimicrobial. Many valuable compounds extracted from C. asiatica by SFME showed that this method is a promising green method with faster extraction.…”

“…The presence of cyclohexane in C. asiatica is expected since most of the polyacetylene compounds were found in the Apiaceae family. A study by Bhuyar et al [17] found hexadecanoic acid as a major compound in C. asiatica extract. The extract was high in fatty acids, which is in agreement with a study by Ogunka-Nnoka [18], who detected 78.48 % saturated and 21.53 % unsaturated.…”

VALORIZATION OF PERILLALDEHYDE MOLECULE CONTAINED IN THE ESSENTIAL OIL OF Ammodaucus Leucotrichus Coss. FROM THE SAHARAN ZONES OF MOROCCO

“…More than two-thirds of the drugs currently used in cancer treatments come directly from natural products (Gurnani et al, 2014). Much recent researches have great interest in screening of medicinal plants for new therapeutics (Bhuyar et al 2021). Because of their efficacy, safety, and availability (Naczk et al, 2006).…”

“…Because of their efficacy, safety, and availability (Naczk et al, 2006). The secondary metabolites existed in plants are manly used as active principals of many drags (Bhuyar et al 2021). Ammodaucus leucotrichus Coss belongs to the apiaceae family and Ammodaucus genus.…”

Biotic Relations Between Pathogenic Leptospires And Green Algae

“…It is known that green algae often act as a dominant group of plants in freshwater plankton. Besides, there is a variety of different biologically active substances (Hasbi et al, 2021;Bhuyar et al, 2020). However, despite this fact, the researches on the interactions of various species of green algae with pathogenic leptospira are extremely insufficient.…”